Immunogenicity and protective efficacy of a new Leishmania hypothetical protein applied as a DNA vaccine or in a recombinant form against Leishmania infantum infection.
dc.contributor.author | Ribeiro, Patrícia Aparecida Fernandes | |
dc.contributor.author | Dias, Daniel Silva | |
dc.contributor.author | Lage, Daniela Pagliara | |
dc.contributor.author | Martins, Vivian Tamietti | |
dc.contributor.author | Costa, Lourena Emanuele | |
dc.contributor.author | Santos, Thaís Teodoro de Oliveira | |
dc.contributor.author | Ramos, Fernanda Fonseca | |
dc.contributor.author | Tavares, Grasiele de Sousa Vieira | |
dc.contributor.author | Mendonça, Débora Vasconcelos Costa | |
dc.contributor.author | Ribeiro, Fernanda Ludolf | |
dc.contributor.author | Gomes, Dawidson Assis | |
dc.contributor.author | Rodrigues, Michele Angela | |
dc.contributor.author | Chávez Fumagalli, Miguel Angel | |
dc.contributor.author | Silva, Eduardo Sergio da | |
dc.contributor.author | Galdino, Alexsandro Sobreira | |
dc.contributor.author | Duarte, Mariana Costa | |
dc.contributor.author | Roatt, Bruno Mendes | |
dc.contributor.author | Souza, Daniel Menezes | |
dc.contributor.author | Teixeira Junior, Antonio Lucio | |
dc.contributor.author | Coelho, Eduardo Antônio Ferraz | |
dc.date.accessioned | 2020-03-17T13:51:38Z | |
dc.date.available | 2020-03-17T13:51:38Z | |
dc.date.issued | 2019 | |
dc.description.abstract | Vaccination is one the most important strategies for the prevention of visceral leishmaniasis (VL). In the current study, a new Leishmania hypothetical protein, LiHyP, which was previously showed as antigenic in an immunoproteomic search in canine VL, was evaluated regarding its immunogenicity and protective efficacy against Leishmania infantum infection. The effects of the immunization using LiHyP were evaluated when administered as a DNA plasmid (DNA LiHyP) or recombinant protein (rLiHyP) associated with saponin. The immunity elicited by both vaccination regimens reduced the parasitism in liver, spleen, bone marrow and draining lymph nodes, being associated with high levels of IFN-γ, IL-12, GM-CSF, and specific IgG2a antibody, besides low production of IL-4, IL-10, and protein and parasite-specific IgG1 antibodies. CD4+ T cells contributed more significantly to IFN-γ production in the rLiHyP/saponin group, while CD8+ T cells were more important in the production of this cytokine in the DNA LiHyP group. In addition, increased IFN-γ secretion, along with low levels of IL-10, were found when PBMCs from treated VL subject and healthy individuals were stimulated with the recombinant protein. In conclusion, when administered either as a DNA plasmid or recombinant protein, LiHyP can direct the immune response towards a Th1 immune profile, protecting animals against L. infantum infection; therefore, it can be seen as a promising immunogen against human VL. | pt_BR |
dc.identifier.citation | RIBEIRO, P. A. F. et al. Immunogenicity and protective efficacy of a new Leishmania hypothetical protein applied as a DNA vaccine or in a recombinant form against Leishmania infantum infection. Molecular Immunology, v. 106, p. 108-118, fev. 2019. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0161589018310563?via%3Dihub>. Acesso em: 10 fev. 2020. | pt_BR |
dc.identifier.doi | https://doi.org/10.1016/j.molimm.2018.12.025 | pt_BR |
dc.identifier.issn | 0161-5890 | |
dc.identifier.uri | http://www.repositorio.ufop.br/handle/123456789/12001 | |
dc.identifier.uri2 | https://www.sciencedirect.com/science/article/pii/S0161589018310563?via%3Dihub | pt_BR |
dc.language.iso | en_US | pt_BR |
dc.rights | restrito | pt_BR |
dc.subject | Immune response | pt_BR |
dc.title | Immunogenicity and protective efficacy of a new Leishmania hypothetical protein applied as a DNA vaccine or in a recombinant form against Leishmania infantum infection. | pt_BR |
dc.type | Artigo publicado em periodico | pt_BR |
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