DEFAR - Departamento de Farmácia
URI permanente desta comunidadehttp://www.hml.repositorio.ufop.br/handle/123456789/530
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2 resultados
Resultados da Pesquisa
Item Modulation of long non-coding RNAs by different classes of secondary metabolites from plants : a mini-review on antitumor effects.(2022) Almeida, Tamires Cunha; Seibert, Janaína Brandão; Amparo, Tatiane Roquete; Souza, Gustavo Henrique Bianco de; Silva, Glenda Nicioli da; Santos, Orlando David Henrique dosThe broad pharmacological spectrum of plants is related to their secondary metabolism, which is responsible for the synthesis of different compounds that have multiple effects on cellular physiology. Among the biological effects presented by phytochemicals, their use for the prevention and treatment of cancer can be highlighted. This occurs due to several mechanisms of antitumor ac- tion demonstrated by these compounds, including regulation of the cell signaling pathways and inhibi- tion of tumor growth. In this way, long non-coding RNAs (lncRNAs) appear to be promising targets for the treatment of cancer. Their deregulation has already been related to a variety of clinical- pathological parameters. However, the effects of secondary metabolites on lncRNAs are still restrict- ed. For this reason, the present review aimed to gather data on phytochemicals with action on lncRNAs in order to confirm their possible antitumor potential. According to the literature, terpenoid and flavonoid are the main examples of secondary metabolites involved with lncRNAs activity. In addition, the lncRNAs H19, CASC2, HOTAIR, NKILA, CCAT1, MALAT1, AFAP1-AS1, MEG3, and CDKN2B-AS1 can be highlighted as important targets in the search for new anti-tumor agents since they act as modulating pathways related to cell proliferation, cell cycle, apoptosis, cell migration and invasion. Finally, challenges for the use of natural products as a commercial drug were also discussed. The low yield, selectivity index and undesirable pharmacokinetic parameters were emphasized as a difficulty for obtaining these compounds on a large scale and for improving the potency of its biologi- cal effect. However, the synthesis and/or development of formulations were suggested as a possible approach to solve these problems. All of these data together confirm the potential of secondary me- tabolites as a source of new anti-tumor agents acting on lncRNAs.Item Cytotoxic, antitumor and toxicological profile of passiflora alata leaf extract.(2020) Amaral, Ricardo Guimarães; Gomes, Silvana Vieira Floresta; Andrade, Luciana Nalone; Santos, Sara Albuquerque dos; Severino, Patrícia; Albuquerque Júnior, Ricardo Luiz Cavalcanti de; Souto, Eliana B.; Brandão, Geraldo Célio; Santos, Sandra Lauton; David, Jorge Mauricio; Carvalho, Adriana AndradePassiflora alata or passion fruit is a native flowering plant from Amazon, geographically spread from Peru to Brazil. The plant has long been used in folks medicine for its pharmacological properties and is included in the Brazilian Pharmacopoeia since 1929. The aim of this study was to evaluate the potential cytotoxic and antitumor activities of Passiflora alata leaf extract (PaLE) in S180-tumor bearing mice. The percentage of cell proliferation inhibition (% CPI) and IC50 in relation to 4 tumor cell lines were determined in PC3, K-562, HepG2 and S180 cell lines using the MTT assay. PaLE showed a CPI > 75% and greater potency (IC50 < 30 µg/mL) against PC3 and S180 cell lines. PaLE showed antitumor activity in treatments intraperitoneally (36.75% and 44.99% at doses of 100 and 150 mg/kg/day, respectively). Toxicological changes were shown in the reduced body mass associated with reduced food consumption, increased spleen mass associated with histopathological increase in the white pulp of the spleen and increased number of total leukocytes with changes in the percentage relationship between lymphocytes and neutrophils. Our outcomes corroborate the conclusion that PaLE has antitumor activity in vitro and in vivo with low toxicity.