DEFAR - Departamento de Farmácia
URI permanente desta comunidadehttp://www.hml.repositorio.ufop.br/handle/123456789/530
Navegar
3 resultados
Resultados da Pesquisa
Item Recent progress in micro and nano-encapsulation of bioactive derivatives of the Brazilian genus Pterodon.(2021) Lemos, Janaina de Alcantara; Oliveira, Anna Eliza M. F. M.; Araújo, Raquel Silva; Townsend, Danyelle M.; Ferreira, Lucas Antônio Miranda; Barros, André Luís Branco deIn the last few decades, utilization of medicinal plants by the pharmaceutical industry has led to the identifi- cation of many new bioactive compounds. The genus Pterodon, native of the Brazilian Flora, is known for the therapeutic properties attributed to its species, which are widely used in popular medicine for their anti- inflammatory, anti-rheumatic, tonic, and depurative properties. The intrinsic low water solubility of the plant derivatives from the genus, including diterpenes with vouacapane skeletons that are partially associated with the pharmacological activities, impairs the bioavailability of these bioactive compounds. Recent studies have aimed to encapsulate Pterodon products to improve their water solubility, achieve stability, increase their efficacy, and allow clinical applications. The purpose of this paper is to review recent research on the use of nanotechnology for the development of new products from plant derivatives of the Pterodon genus in different types of micro- and nanocarriers. Therapeutic properties of their different products are also presented. Finally, an update about the current and future applications of encapsulated formulations is provided.Item Anti-zika activity of Ouratea semiserrata and dereplication of its constituents.(2021) Ferreira, Gabriel Monteze; Silva, Breno de Mello; Souza, Gustavo Henrique Bianco de; Oliveira, Alaíde Braga de; Brandão, Geraldo CélioZika virus is an arbovirus that has vector mosquitoes of the genus Aedes. In adult humans, the infection may be asymptomatic or present mild symptoms such as itching and low fever. However, the infection is associated with other severe problems, which encouraged investigations for an effective treatment against this virus. This work evaluated the potential anti-Zika virus effect of the ethanolic extract of Ouratea semiserrata (Mart. & Nees) Engl., Ochnaceae, a medicinal plant popularly used in Brazil for the treatment of viral infections. The extract of the stems was prepared by cold percolation using ethanol as solvent and its content dereplicated by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry. Phenolic com- pounds including rutin, catechin, and epicatechin were identified as the major constituents. The antiviral activity was tested in vitro against Zika virus by the MTT colorimetric method. The ethanol extract inhibited the viral replication cycle with an EC50 of 37.5 μg/ml, and at the concentration of 100 μg/ml, a 100% inhibition of the viral cytopathic effect was obtained. Rutin and epicatechin inhibited viral cytopathic effect in Vero cells with EC50 > 50.00 μg/ml.Item Cytotoxic, antitumor and toxicological profile of passiflora alata leaf extract.(2020) Amaral, Ricardo Guimarães; Gomes, Silvana Vieira Floresta; Andrade, Luciana Nalone; Santos, Sara Albuquerque dos; Severino, Patrícia; Albuquerque Júnior, Ricardo Luiz Cavalcanti de; Souto, Eliana B.; Brandão, Geraldo Célio; Santos, Sandra Lauton; David, Jorge Mauricio; Carvalho, Adriana AndradePassiflora alata or passion fruit is a native flowering plant from Amazon, geographically spread from Peru to Brazil. The plant has long been used in folks medicine for its pharmacological properties and is included in the Brazilian Pharmacopoeia since 1929. The aim of this study was to evaluate the potential cytotoxic and antitumor activities of Passiflora alata leaf extract (PaLE) in S180-tumor bearing mice. The percentage of cell proliferation inhibition (% CPI) and IC50 in relation to 4 tumor cell lines were determined in PC3, K-562, HepG2 and S180 cell lines using the MTT assay. PaLE showed a CPI > 75% and greater potency (IC50 < 30 µg/mL) against PC3 and S180 cell lines. PaLE showed antitumor activity in treatments intraperitoneally (36.75% and 44.99% at doses of 100 and 150 mg/kg/day, respectively). Toxicological changes were shown in the reduced body mass associated with reduced food consumption, increased spleen mass associated with histopathological increase in the white pulp of the spleen and increased number of total leukocytes with changes in the percentage relationship between lymphocytes and neutrophils. Our outcomes corroborate the conclusion that PaLE has antitumor activity in vitro and in vivo with low toxicity.