DEFAR - Departamento de Farmácia
URI permanente desta comunidadehttp://www.hml.repositorio.ufop.br/handle/123456789/530
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6 resultados
Resultados da Pesquisa
Item Flavonol triglycosides of leaves from Maytenus robusta with acetylcholinesterase inhibition.(2017) Sousa, Grasiely Faria de; Aguiar, Mariana Guerra de; Takahashi, Jacqueline Aparecida; Alves, Tânia Maria de Almeida; Kohlhoff, Markus; Vieira Filho, Sidney Augusto; Silva, Grácia Divina de Fátima; Duarte, Lucienir PainsAlthough Maytenus robusta aqueous infusions of leaves are used in Brazilian traditional medicine for stomach disease treatment, only a few chemical studies of this species are found in literature. The phytochemical investigation of methanol extract from M. robusta leaves yielded the known compound kaempferol (3) and two new flavonol glycosides: kaempferol-3-O-b-D-glucopyranosyl-(1 !3)-a-Lrhamnopyranosyl-( 1 ! 2)-b-D-glucopyranoside (1) and quercetin-3-O-b-D-glucopyranosyl-(1 !3)-a-Lrhamnopyranosyl-( 1 ! 2)-b-D-glucopyranoside (2). The chemical structures of 1 and 2 were elucidated by 1D/2D NMR, ESI–MS and ESI–MS2 spectral data. It is the first time flavonoids have been reported from M. robusta. Flavonols 1 and 2 showed 66% and 80% acetylcholinesterase (AChE) inhibition, compared to 93% of the standard eserine, by the Ellman’s method. These substances are one of the few active flavonols linked to a trisaccharide chain in the literature presenting this activity, and contribute to the screening for new types of natural AChE inhibitors.Item Constituents of fruit pulp of Maytenus salicifolia and complete 1D/2D NMR data of 3beta-hydroxy-D-B-friedo-olean-5-ene.(2010) Valladão, Frederico Nunes; Miranda, Roqueline Rodrigues Silva de; Oliveira, Gabriela Soares de; Silva, Grácia Divina de Fátima; Duarte, Lucienir Pains; Vieira Filho, Sidney AugustoA mixture of long-chain hydrocarbons constituted by nonacosane (29C, 7.5%), hentriacontane (31C, 48.3%), and tritriacontane (33C, 30.1%), the ester 1 -acetyloxymethylpentacosa-20 -enyl 10-hydroxydecanoate (2), -amyrin (3), friedelin (4), and lupeol (5), and 3 -hydroxy-D:B-friedo-olean-5-ene (6) were identified as constituents of fruits of Maytenus salicifolia Reissek (Celastraceae). The structural formula and the stereochemistry of compound 6 were established by the data obtained through 1H and 13C NMR spectroscopy, including DEPT-135 and 2D (HMQC, HMBC, and NOESY) experiments. By analysis of the spectral data, it was possible to correct seven chemical shift assignments of compound 6, which were erroneous attributed and published in the scientific literature.Item Antigiardial activity of the hexane extract of Maytenus gonoclada mart.(2012) Silva, Fernando César; Busatti, Haendel Gonçalves Nogueira Oliveira; Gomes, Maria Aparecida; Duarte, Lucienir Pains; Silva, Grácia Divina de Fátima; Vieira Filho, Sidney AugustoPlants of the Maytenus genus have been used in folk medicine in Brazil for treatment of gastrointestinal motility disturbances such as diarrhea. The present study was performed to evaluate antigiardial activity of the hexane extract and triterpenes isolated from the branches of Maytenus gonoclada Mart. (Celastraceae). The hexane extract and triterpenes were examined for antigiardial activity using the colorimetric method against Giardia lamblia (ATCC 30888). The hexane extract exhibited antigiardial activity. This result may partly explain and support the use of plants of the Maytenus genus for the treatment of diarrhea in folk medicine in Brazil.Item Pentacyclic triterpenes from branches of Maytenus robusta and in vitro cytotoxic property against 4T1 cancer cells.(2014) Sousa, Grasiely Faria de; Soares, Daniel Crístian Ferreira; Mussel, Wagner da Nova; Pompeu, Nana Flora Elias; Silva, Grácia Divina de Fátima; Vieira Filho, Sidney Augusto; Duarte, Lucienir PainsDois novos friedelanos, 1 e 2, e cinco triterpenos pentacíclicos conhecidos foram isolados dos galhos de Maytenus robusta. Suas estruturas químicas foram identificadas como 3,16-dioxo-29- hidroxifriedelano (1), 3-oxo-16b,29-di-hidroxifriedelano (2), 3-oxofriedelano (3), 3b-friedelinol (4), 3,16-dioxofriedelano (5), 3-oxo-29-hidroxifriedelano (6) e 3,16-dioxo-12a-hidroxifriedelano (7). A estrutura e estereoquímica dos triterpenos 1 e 2 foram estabelecidas por infravermelho (IR), ressonância magnética nuclear (NMR) 1D/2D, espectrometria de massas de alta resolução com ionização química à pressão atmosférica (HR-APCIMS) e difração de raios X de pó. A atividade citotóxica in vitro dos triterpenos 1 a 6 foi avaliada frente a células de câncer de mama murino. Os triterpenos 1 e 2 apresentaram atividade citotóxica contra células 4T1 em baixa concentração.Item Maytenus salicifolia : triterpenes isolated from stems and antioxidant property of extracts from aerial parts.(2011) Magalhães, Cássia Gonçalves; Ferrari, Fernanda Cristina; Guimarães, Dênia Antunes Saúde; Silva, Grácia Divina de Fátima; Duarte, Lucienir Pains; Figueiredo, Rute Cunha; Vieira Filho, Sidney AugustoSix pentacyclic triterpenes were isolated from hexane extract of stems of Maytenus salicifolia Reissek, Celastraceae: 30-hydroxyfriedelan-3-one (1), 3,16-dioxofriedelane (2), friedeline (3), lupeol (4), betuline (5) and lup-20(29)- en-3,30-diol (6). The structure each one was established on the basis of detailed 1H and 13C NMR spectral investigation and by comparison with the respective literature values. For compound 1, the complete 2D NMR (HMBC, HMQC and NOESY) spectral data were herein reported for the first time. Compounds 1, 2, 5 and 6 were isolated for the first time from this plant. Antioxidant activity is described for some extracts from species of the Celastraceae family, then, the extracts from aerial parts of M. salicifolia were evaluated in relation to antioxidant potential using the DPPH method. Compared to quecertin, the AcEt extract (EAF) from leaves, AcEt (EAPF) and MeOH (EMPF) from pulp fruit and AcEt (EAT) and MeOH (EMT) from stems showed significant antioxidant property.Item Evaluation of antimicrobial activity and toxic potential of extracts and triterpenes isolated from Maytenus imbricata.(2012) Rodrigues, Vanessa Gregório; Duarte, Lucienir Pains; Silva, Grácia Divina de Fátima; Silva, Fernando César; Góes, Jefferson Vieira de; Takahashi, Jacqueline Aparecida; Pimenta, Lúcia Pinheiro Santos; Vieira Filho, Sidney AugustoThe phytochemical study of hexane/ethyl ether (1:1) extract of the roots of M. imbricata, Celastraceae, resulted in the isolation and characterization of six known triterpenes: 11α-hydroxylup-20(29)-en-3-one, previously isolated from this species besides, 3β,11α- di-hydroxylup-20(29)-ene, 3,7-dioxofriedelane, 3-oxo-29-hydroxyfriedelane, tingenone and 6-oxo-tingenol. The chemical structures of these triterpenes were established by spectrometric data (IR, 1H and 13C NMR) and through comparison with literature data. The hexane/ethyl ether (1:1), ethyl acetate and methanol extracts, and 11α-hydroxylup-20(29)-en-3-one, tingenone and 6-oxo-tingenol, showed antimicrobial properties on in vitro assays. All extracts and triterpenes, except 3β,11α-di-hydroxylup-20(29)-ene, presented toxicity demonstrated by the larvicidal effect test using Artemia salina.