DEFAR - Departamento de Farmácia

URI permanente desta comunidadehttp://www.hml.repositorio.ufop.br/handle/123456789/530

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2020 - 20222

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Autor: search.filters.author.Souza, Gustavo Henrique Bianco deAssunto: search.filters.subject.Photoprotection

Resultados da Pesquisa

Agora exibindo 1 - 2 de 2
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    In vitro and in silico investigation of the photoprotective and antioxidant potential of Protium spruceanum leaves and its main flavonoids.
    (2022) Amparo, Tatiane Roquete; Silva, Anne Cherem Peixoto da; Seibert, Janaína Brandão; Silva, Débora dos Santos da; Santos, Viviane Martins Rebello dos; Vieira, Paula Melo de Abreu; Brandão, Geraldo Célio; Souza, Gustavo Henrique Bianco de; Santos, Bianca Aloise Maneira Corrêa
    For the first time, the photoprotective potential (UV absorption and antioxidant properties) of Protium spru- ceanum leaves and its main flavonoids was investigated combining an in vitro and in silico approach. The extract was standardized in rutin content (0.8% w/w), quantified by a validated HPLC-DAD method. The extract pro- tected fibroblasts and keratinocytes against cell death in the stress oxidative H2O2-induced test, with greater effect than rutin standard. This cytoprotection is correlated to the free radical scavenging action, determined by DPPH method. The theoretical investigation on the UV absorption showed an excellent correlation of the results (R2 = 0.911) allowing valuable information about the photoprotective properties of rutin, afzelin and quercitrin, as the importance of the A, B and C rings in the UV absorption property and the hydroxyls in B ring (catechol group) leading to a better antioxidant action. Therefore, the extract from P. spruceanum leaves is a promising candidate for sunscreen development.
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    Melaleuca leucadendron (L.) L. flower extract exhibits antioxidant and photoprotective activities in human keratinocytes exposed to ultraviolet B radiation.
    (2020) Silva, Luan Silvestro Bianchini; Perasoli, Fernanda Barçante; Carvalho, Karen Vitor; Vieira, Karla Murata; Lopes, Miriam Teresa Paz; Souza, Gustavo Henrique Bianco de; Santos, Orlando David Henrique dos; Freitas, Katia Michelle
    Recently, there has been a demand for the replacement of chemical sunscreens with natural compounds that could prevent or restore UV-induced skin damage. Here, we investigated the photoprotective influence of the Melaleuca leucadendron ethanolic flower extract (EEMec) on factors involved in cellular and molecular UVBinduced oxidative stress in human skin keratinocytes (HaCaT). The phytochemical constituents, antioxidant potential by DPPH assay, content of total phenolic and flavonoid compounds in EEMec were evaluated. HaCaT cells were treated with EEMec followed by irradiation with UVB. CAT activity; GSH and ROS levels; and SOD1, GPx, CAT and COX-2 expression assays were employed to verify the oxidative stress, as well as EEMec effect on transmembrane transport, and pro-inflammatory and pro-apoptotic protein expression. EEMec reverted the viability loss of HaCaT cells after irradiation with UVB, exhibited significant antioxidant capacity and free radical scavenging activity in vitro, inhibited COX-2 expression and ensure protection of DNA-damage. EEMec shown a great photoprotective property to prevent keratinocytes damage induced by UV radiation and, thus a candidate potential to application as an adjuvant in sunscreen formulations as a strategy to reduce risk of sunburn and prevent skin diseases associated with UV-induced inflammation and cancer.