DEFAR - Departamento de Farmácia
URI permanente desta comunidadehttp://www.hml.repositorio.ufop.br/handle/123456789/530
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7 resultados
Resultados da Pesquisa
Item Modulation of long non-coding RNAs by different classes of secondary metabolites from plants : a mini-review on antitumor effects.(2022) Almeida, Tamires Cunha; Seibert, Janaína Brandão; Amparo, Tatiane Roquete; Souza, Gustavo Henrique Bianco de; Silva, Glenda Nicioli da; Santos, Orlando David Henrique dosThe broad pharmacological spectrum of plants is related to their secondary metabolism, which is responsible for the synthesis of different compounds that have multiple effects on cellular physiology. Among the biological effects presented by phytochemicals, their use for the prevention and treatment of cancer can be highlighted. This occurs due to several mechanisms of antitumor ac- tion demonstrated by these compounds, including regulation of the cell signaling pathways and inhibi- tion of tumor growth. In this way, long non-coding RNAs (lncRNAs) appear to be promising targets for the treatment of cancer. Their deregulation has already been related to a variety of clinical- pathological parameters. However, the effects of secondary metabolites on lncRNAs are still restrict- ed. For this reason, the present review aimed to gather data on phytochemicals with action on lncRNAs in order to confirm their possible antitumor potential. According to the literature, terpenoid and flavonoid are the main examples of secondary metabolites involved with lncRNAs activity. In addition, the lncRNAs H19, CASC2, HOTAIR, NKILA, CCAT1, MALAT1, AFAP1-AS1, MEG3, and CDKN2B-AS1 can be highlighted as important targets in the search for new anti-tumor agents since they act as modulating pathways related to cell proliferation, cell cycle, apoptosis, cell migration and invasion. Finally, challenges for the use of natural products as a commercial drug were also discussed. The low yield, selectivity index and undesirable pharmacokinetic parameters were emphasized as a difficulty for obtaining these compounds on a large scale and for improving the potency of its biologi- cal effect. However, the synthesis and/or development of formulations were suggested as a possible approach to solve these problems. All of these data together confirm the potential of secondary me- tabolites as a source of new anti-tumor agents acting on lncRNAs.Item Chemical constituents, antioxidant potential, antibacterial study and photoprotective activity of Brazilian corn silk extract.(2022) Azevedo, Amanda Scofield de; Seibert, Janaína Brandão; Amparo, Tatiane Roquete; Antunes, Amanda dos Santos; Sousa, Lucas Resende Dutra; Souza, Gustavo Henrique Bianco de; Teixeira, Luiz Fernando de Medeiros; Vieira, Paula Melo de Abreu; Santos, Viviane Martins Rebello dos; Nascimento, Adriana Mendes do; Nascimento, Andréa Mendes doThe aim of this work was to determine the total phenolic content and evaluate the antioxidant, antibacterial, photoprotective and cytotoxic properties of corn silk ethanol extract prepared from corn silk that was air-dried at room temperature. Corn silk ethanol extract has a high content of phenolic compounds. The concentrations of ethanol extract required to produce 50% of maximal effect (EC50) in DPPH and ABTS radical scavenging assays were 489.0 and 166.1 μg/mL respectively, indicating low activity when compared to the positive control. The ethanolic extract of corn silk showed antibacterial activity, mainly on Gram-positive bacteria. The in vitro sun protection factor (SPF) of the ethanolic extract of corn silk incorporated into sunscreen UVA-UVB 5% gel with Pemulen TR-1® was evaluated for the first time. A relative increase in SPF efficacy was observed for the extract when the sunscreen was incorporated. The ethanolic extract of corn silk and the gel were considered non-cytotoxic. The corn silk ethanolic extract was characterized using ultra performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS). Eleven compounds including flavonoids, coumaric acid derivatives, terpenoid, hydroxycinnamic acid derivative and quinic acid derivative were suggested based on their accurate mass and MS/MS spectra.Item In vitro and in silico investigation of the photoprotective and antioxidant potential of Protium spruceanum leaves and its main flavonoids.(2022) Amparo, Tatiane Roquete; Silva, Anne Cherem Peixoto da; Seibert, Janaína Brandão; Silva, Débora dos Santos da; Santos, Viviane Martins Rebello dos; Vieira, Paula Melo de Abreu; Brandão, Geraldo Célio; Souza, Gustavo Henrique Bianco de; Santos, Bianca Aloise Maneira CorrêaFor the first time, the photoprotective potential (UV absorption and antioxidant properties) of Protium spru- ceanum leaves and its main flavonoids was investigated combining an in vitro and in silico approach. The extract was standardized in rutin content (0.8% w/w), quantified by a validated HPLC-DAD method. The extract pro- tected fibroblasts and keratinocytes against cell death in the stress oxidative H2O2-induced test, with greater effect than rutin standard. This cytoprotection is correlated to the free radical scavenging action, determined by DPPH method. The theoretical investigation on the UV absorption showed an excellent correlation of the results (R2 = 0.911) allowing valuable information about the photoprotective properties of rutin, afzelin and quercitrin, as the importance of the A, B and C rings in the UV absorption property and the hydroxyls in B ring (catechol group) leading to a better antioxidant action. Therefore, the extract from P. spruceanum leaves is a promising candidate for sunscreen development.Item Chemical constituents and allelopathic activity of the essential oil from leaves of Eremanthus erythropappus.(2018) Pinto, Ana Paula Resende; Seibert, Janaína Brandão; Santos, Orlando David Henrique dos; Vieira Filho, Sidney Augusto; Nascimento, Andréa Mendes doThe essential oil from leaves of Eremanthus erythropappus (DC.) MacLeish (Asteraceae) collected in South-eastern Brazil was extracted using a combination of water and steam distillation and investigated by chromatography/mass spectrometry (GC-MS). The sesquiterpene hydrocarbons were predominant (46.6%), followed by oxygenated sesquiterpenes (29.3%) and monoterpene hydrocarbons (18.8%). The major compounds were (β)-caryophyllene (29.3%), caryophyllene oxide (22.1%) and β-pinene (12.8%). The allelopathic activity of E. erythropappus essential oil on the seed germination and radicle length of seedlings was evaluated in lettuce (Lactuca sativa L.), tomato (Lycopersicum esculentum Mill.) and in two field weeds (field mustard – Brassica rapa L. and hairy beggarticks – Bidens pilosa L.). Among the weed species tested, the most significant inhibition of seed germination was observed in field mustard, as measured by the half–minimal inhibitory concentration, IC50 (IC50 = 26.5 μL mL–1), and the most significant inhibitory effect on radicle length of seedlings was observed in seedlings of hairy beggarticks (IC50 = 16.3 μL mL–1). In contrast, the lowest allelopathic effects of E. erythropappus essential oil on seed germination and radicle length of seedlings were observed in tomato (IC50 = >200 μL mL–1 and 130.1 μL mL–1 respectively) and lettuce (IC50 = 97.1 μL mL–1 and 35.1 μL mL–1 respectively). These results revealed significant allelopathic potential of E. erythropappus essential oil against weeds, but minimal effect on lettuce and tomato germination, and thus suggests it is feasible to use E. erythropappus sustainability as an eco-friendly bioherbicide in cropping settings.Item Seasonality study of essential oil from leaves of Cymbopogon densiflorus and nanoemulsion development with antioxidant activity.(2019) Seibert, Janaína Brandão; Rodrigues, Ivanildes Vasconcelos; Carneiro, Simone Pinto; Amparo, Tatiane Roquete; Lanza, Juliane Sousa; Frezard, Frederic Jean Georges; Souza, Gustavo Henrique Bianco de; Santos, Orlando David Henrique dosThe development of formulations that maintain the biological and physical chemistry properties of essential oils is an important choice when they are used as an active ingredient. This study aimed to characterize the essential oil from leaves of Cymbopogon densiflorus and evaluate the antioxidant activity of the oil, and to produce a nanoemulsion formulation containing it. The essential oil was obtained by hydrodistillation, and seasonality was analysed every 2 months by gas chromatography–mass spectrometry, showing that more than 90% of the composition was maintained for the whole period and that the major compounds were trans‐p‐menta‐2,8‐dien‐1‐ol, cis‐p‐menta‐2,8‐dien‐1‐ol, trans‐p‐menta‐1(7),8‐dien‐2‐ol, cis‐piperitol, and cis‐p‐menta‐1(7),8‐dien‐2‐ol. Stable nanoemulsions were prepared by phase inversion method encapsulating the essential oil. The antioxidant activity was evaluated by 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) free radical scavenging and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulphonic acid (ABTS) methods. In the first test, free and nanoemulsified essential oil showed half‐maximal inhibitory concentration (IC50) values equivalent to 14.689 and 3.692 mg mL−1, respectively. In the second test, these values were 0.567 and 0.43 mg mL−1. The development of nanoemulsion‐based essential oil from leaves of C. densiflorus was viable, and the formulated oil was able to reproduce the antioxidant activity at a concentration four times lower than that of the pure essential oil.Item Development of propolis nanoemulsion with antioxidant and antimicrobial activity for use as a potential natural preservative.(2019) Seibert, Janaína Brandão; Silva, Jessica Paola Bautista; Amparo, Tatiane Roquete; Petit, Alicia; Pervier, Pauline; Almeida, Juliana Cristina dos Santos; Azevedo, Mariana Costa; Silveira, Benila Maria; Brandão, Geraldo Célio; Souza, Gustavo Henrique Bianco de; Teixeira, Luiz Fernando de Medeiros; Santos, Orlando David Henrique dosPropolis has demonstrated potential use as food preservative but it presents strong and unpleasant flavor that alters the sensory characteristics foods. A nanoemulsion was proposed to carry the Brazilian propolis extracts for use as natural food preservative. Antimicrobial and antioxidant activities and chemical constituents of the extracts were investigated. The latter were made by sequential extraction using different solvents (hexane, ethyl acetate and ethanol). Antimicrobial activity was evaluated by agar diffusion and microdilution methods and antioxidant activity by DPPH and ABTS assays. Extracts showed antibacterial and antioxidant activity, highlighting the ethanolic which contained artepillin-C, kaempferide, drupanin and p-coumaric acid as main compounds by LC-MS analysis. The nanoemulsion developed by phase inversion method was characterized and stable under thermal-stress and centrifugation conditions. Biological properties evaluated were effectively maintained by the formulation. It was concluded that the nanoemulsion can be used as a food preservative, preventing degradation and masking the propolis off-flavor.Item Antibacterial activity of extract and fractions from branches of Protium spruceanum and cytotoxicity on fibroblasts.(2017) Amparo, Tatiane Roquete; Rodrigues, Ivanildes Vasconcelos; Seibert, Janaína Brandão; Souza, Rafaella Hilda Zaniti; Oliveira, Amanda Ribeiro de; Cabral, Vivette Appolinário Rodrigues; Vieira, Paula Melo de Abreu; Brandão, Geraldo Célio; Okuma, Adriana Akemi; Vieira Filho, Sidney Augusto; Teixeira, Luiz Fernando de Medeiros; Souza, Gustavo Henrique Bianco deThe crude ethanol extract (CEE) and fractions from branches of Protium spruceanum were subjected to antibacterial and cytotoxicity assays. Compounds of the most active fraction were identified by GC-MS and LC-MS. CEE was active against 19 bacteria and the ethyl acetate fraction (EAF) showed the lowest minimum bactericidal concentration (MBC 0.3–80.0 mg/mL). Through time-kill assay was observed that EAF induced rapid bactericidal effect against Staphylococcus saprophyticus. The cytotoxicity tests against L929 fibroblasts showed great potential of EAF on the treatment of infections caused by five bacteria (MBC < IC50). The results provide in vitro scientific support to the possible application of branches of P. spruceanum as antimicrobial agent that may contribute for treatment of infections.