DEFAR - Departamento de Farmácia
URI permanente desta comunidadehttp://www.hml.repositorio.ufop.br/handle/123456789/530
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Resultados da Pesquisa
Item Brine shrimp (Artemia salina Leach) bioassay of extracts from Lychnophoriopsis candelabrum and different Lychnophora species.(2012) Ferraz Filha, Zilma Schimith; Lombardi, Júlio Antônio; Guzzo, Luciana Souza; Guimarães, Dênia Antunes SaúdeO presente estudo teve como objetivo avaliar a citotoxicidade sobre Artemia salina de vinte e dois extratos de cinco espécies do gênero Lychnophora e de uma espécie de Lychnophoriopsis. Os extratos solubilizados em DMSO, preparados nas concentrações finais de 100, 250, 375, 500 e 600 g mL-1, foram adicionados a recipientes contendo náuplios de Artemia salina (10 unidades cada) e completou-se o volume para 5 mL com solução marinha. Lapachol e DMSO 5% foram usados como controles positivo e negativo, respectivamente. As amostras foram mantidas sob iluminação e as larvas mortas foram contadas após 24 horas de contato. O cálculo da LC50 foi feito utilizando-se o programa Probitos. Os extratos etanólicos brutos de cinco espécies apresentaram baixa letalidade na seguinte ordem: Lychnophora trichocarpha (LC50 = 672,38 g mL-1) > Lychnophora pinaster (LC50 = 678,73 g mL-1) > Lychnophora ericoides (LC50 = 738,09 g mL-1) > Lychnophoriopsis candelabrum (LC50 = 812,57 g mL-1) > Lychnophora passerina (LC50 = 921,78 g mL-1). Todos os extratos testados de Lychnophoriopsis candelabrum e o extrato clorofórmico de Lychnophora staavioides mostraram leve toxicidade sobre A. salina. Os resultados indicaram que existem substâncias com potencial atividade farmacológica em todas as espécies testadas.Item Anti-inflammatory activity of Lychnophora passerina, Asteraceae (Brazilian Arnica).(2011) Oliveira, Patricia Capelari de; Paula, Carmem Aparecida de; Rezende, Simone Aparecida; Campos, Fernanda Torres; Guimarães, Andrea Grabe; Lombardi, Júlio Antônio; Guimarães, Dênia Antunes SaúdeEthnopharmacological relevance: Lychnophorapasserina (Asteraceae), popularly known as “arnica,” is used to treat inflammation, pain, rheumatism, contusions, bruises and insect bites in Brazilian traditional medicine. Materials and methods: The anti-inflammatoryactivity of crude ethanolic extract of aerial parts of L. passerina and its ethyl acetate and methanolic fractions had their abilities to modulate the production of NO, TNF-α and IL-10 inflammatory mediators in LPS/IFN-γ-stimulated J774.A1 macrophages evaluated. Moreover, the crude ethanolic extract and derived fractions were also in vivo assayed by carrageenan-induced paw oedema in mice. Results: In vitro assays showed remarkable anti-inflammatoryactivity of L. passerina crude ethanolic extract (EE) and its ethyl acetate (A) and methanolic (M) fractions, through the inhibition of production of NO and TNF-α inflammatory mediators and induction of production of IL-10 anti-inflammatory cytokine. In vivo assays showed anti-inflammatoryactivity for EE 10% ointment, similar to the standard drug diclofenac gel. The A and M fraction ointments 20% presented anti-inflammatoryactivity. Conclusion: The results obtained showed that possible anti-inflammatory effects of EE and its A and M fractions may be attributed to inhibition pro-inflammatory cytokines production, TNF-α and NO and to increased IL-10 production. EE, A and M ointments showed topical in vivoanti-inflammatoryactivity. The in vivoanti-inflammatoryactivity of EE of L. passerina may be related to synergistic effects of different substances in the crude extract. Therefore, traditional use of aerial parts of L. passerina in the inflammatory conditions could be beneficial to treat topical inflammatory conditions, as evidenced by the present study.Item Angiotensin-converting enzyme inhibition by Brazilian plants.(2007) Braga, Fernão Castro; Serra, Carla Penido; Viana Júnior, Nilton de Souza; Oliveira, Alaíde Braga de; Côrtes, Steyner de França; Lombardi, Júlio AntônioThe potential antihypertensive activity of Brazilian plants was evaluated in vitro by its ability to inhibit the angiotensinconverting enzyme (ACE). Forty-four plants belonging to 30 families were investigated. Plants were selected based on their popular use as antihypertensive and/or diuretics. The following plants presented significant ACE inhibition rates: Calophyllum brasiliense, Combretum fruticosum, Leea rubra, Phoenix roebelinii and Terminalia catappa.Item Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts.(2005) Serra, Carla Penido; Côrtes, Steyner de França; Lombardi, Júlio Antônio; Oliveira, Alaíde Braga de; Braga, Fernão CastroAnew method for the in vitro screening of plant extracts with potential angiotensin-converting enzyme (ACE) inhibitory activity is proposed. The method is based on the cleavage of the substrate hippuryl-glycyl-glycine by ACE and subsequent reaction with trinitrobenzenesulfonic acid to form 2,4,6-trinitrophenyl-glycyl-glycine, whose absorbance is determined at 415nm in a microtitre plate reader. Rabbit lung dehydrated by acetone was employed as an enzyme source. Validation of the method showed satisfactory intra-day (CV ¼ 7.63%) and inter-day precision (CV ¼ 13.61%), recovery (97–102.1%), sensitivity (IC50 ¼ 14.1 nmol/l) and linearity in the range 7.5–120 mmol/l of glycyl-glycine (r2 ¼ 0:9921). Besides, the method showed good correlation with a HPLC assay already established for the screening of ACE inhibitors (r ¼ 0:9935 and 0:9034; respectively, for captopril solutions and for plant extracts). The method involves only inexpensive reagents and apparatus.Item Antinociceptive and anti-inflammatory activities of ethanolic extracts of Lychnophora species.(2008) Guzzo, Luciana Souza; Guimarães, Dênia Antunes Saúde; Silva, Adriana Carneiro; Lombardi, Júlio Antônio; Guimarães, Homero Nogueira; Guimarães, Andrea GrabeExtracts from Lychnophora species are traditionally used in Brazil as anti-inflammatory, and to treat bruise, pain and rheumatism. The ethanolic extract of aerial parts of five species of Lychnophoras and one specie of Lychnophoriopsis were examined for the antinociceptive (hot-plate and writhing tests) and anti-inflammatory (carrageenan-induced paw oedema test) activity in mice, by oral and topical routes, respectively. In the hot-plate test, the Lychnophora pinaster (0.75 g/kg) and Lychnophora ericoides (1.50 g/kg) extracts significantly increased the time for licking of the paws. The species Lychnophora passerina, Lychnophoriopsis candelabrum and Lychnophora pinaster, using the dose of 0.75 g/kg, and Lychnophora ericoides and Lychnophora trichocarpha in both doses evaluated (0.75 and 1.50 g/kg) significantly reduced the number of writhes induced by acetic acid. The administration of Lychnophora pinaster and Lychnophora trichocarpha ointments, in both concentrations evaluated (5 and 10%, w/w), and Lychnophora passerina and Lychnophoriopsis candelabrum, in the concentration of 10%, significantly reduced the paw oedema measured 3 h after carrageenan administration, suggesting, for the first time, an anti-inflammatory activity upon topical administration of these species. The present work comparatively demonstrated the antinociceptive and anti-inflammatory activities of some Brazilian Lychnophoras.