DEFAR - Departamento de Farmácia

URI permanente desta comunidadehttp://www.hml.repositorio.ufop.br/handle/123456789/530

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2021 - 20222

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Autor: search.filters.author.Cruz, Rodrigo Alves Soares

Resultados da Pesquisa

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    Lecithin-based nanocapsule loading sucupira (Pterodon emarginatus) oil effects in experimental mucositis.
    (2022) Di Miceli, Jeruza Ferraz Filgueiras; Andrade, Maria Emília Rabelo; Carvalho, Paula Lopes Armond; Santos, Elandia Aparecida; Oliveira, Anna Eliza Maciel de Faria Mota; Fernandes, Caio Pinho; Cruz, Rodrigo Alves Soares; Garrett, Rafael; Mosqueira, Vanessa Carla Furtado; Cassali, Geovanni Dantas; D’Haese, Cecile; Nysten, Bernard; Leite, Jacqueline Isaura Alvarez; Cardoso, Valbert Nascimento; Araújo, Raquel Silva
    Intestinal mucositis (IM) is a frequent adverse effect in anticancer therapy without standard treatment. The oil obtained from sucupira (Pterodon emarginatus) has anti-inflammatory properties, and the soybean lecithin re- duces the intestinal toxicity of several xenobiotics. However, their water insolubility impairs the in vivo appli- cation. For this reason, we evaluated if the nanoencapsulation of sucupira oil (SO) in lecithin-based nanocapsules (SO-NC) could be a therapeutically effective system for the treatment of IM in murine cisplatin (CDDP)-induced intestinal mucositis model. SO was analyzed by LC-HRMS/MS and HPLC. SO-NC was prepared by nano- precipitation and characterized using DLS, HPLC, and AFM. Mice body weight and food consumption were assessed daily during experimental mucositis induced by CDDP. The animals were euthanized, and intestinal permeability, inflammatory mediators, and intestinal histology were performed. SO-NC demonstrated adequate characteristics for oral administration as size under 300 nm, IP < 0.3, high EE, and spherical shape. In vitro cytotoxicity performed against RAW 264.7 cell lines resulted in cell viability above 80 % confirming the non- cytotoxic profile of SO (IC50 268 μg/mL) and SO-NC (IC50 118.5 μg/mL) up to 117.2 μg/mL. The untreated mice showed intestinal toxicity after i.p. of CDDP, principally weight loss, increased intestinal permeability, and MPO and TNF-α levels. Surprisingly, the administration of SO to CDDP-mucositis animals did not circumvent the CDDP effects and increased intestinal permeability. However, SO-NC proved efficient in mitigating the experi- mental intestinal mucositis by improving intestinal epithelium architecture, reducing intestinal permeability, and improving the MPO levels. In conclusion, SO-NC can positively impact intestinal mucositis by promoting mucosal recovery. This is a promising strategy for developing a new treatment for intestinal mucositis.
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    Nano-emulsions of the essential oil of Baccharis reticularia and its constituents as eco-friendly repellents against Tribolium castaneum.
    (2021) Lima, Luana A.; Sá, Paula S. Ferreira; Garcia Júnior, Manoel Daltro Nunes; Pereira, Vera Lúcia P.; Carvalho, José Carlos Tavares; Rocha, Leandro; Fernandes, Caio Pinho; Souto, Raimundo Nonato Picanço; Araújo, Raquel Silva; Botas, Gisele da Silva; Cruz, Rodrigo Alves Soares
    Nanoproducts based on natural products have been studied as ecologically and economically sustainable alter- natives for crop pest control. Baccharis reticularia DC. is a shrub endemic to Brazil, which leaves are traditionally used in aromatic medicinal baths. This study aims to evaluate the repellent activities of nano-emulsions con- taining the B. reticularia phytochemically characterized essential oil and some of its monoterpene constituents (limonene, α-pinene, and β-pinene) against Tribolium castaneum (Herbst). Essential oil was obtained by hydro- distillation of fresh leaves and stems and yielded 0.28 % (w/w). The main constituents of the essential oil were: limonene (14.6 %), β-myrcene (12.6 %), and bicyclogermacrene (12.5 %). The required hydrophile-lipophile balance (rHLB) of the nano-emulsions were: 13 (α-pinene) and 15 (essential oil, limonene, and β-pinene). All nano-emulsions showed mean droplet sizes bellow 200 nm and remain stable, at least, for 28 days (limonene), 90 days (β-pinene), or 150 days (essential oil and α-pinene). All nano-emulsions exhibited repellent activity against T. castaneum at 8.8 μg/cm2 . Limonene and α-pinene were the most active nano-emulsions, showing significant repellent action at 1.1 μg/cm2 , which indicates that these compounds contribute to the repellent action of the essential oil of B. reticularia. The present study opens perspectives for the utilization of B. reticularia essential oil and its constituents as raw material of new promising nanopesticides produced using a low energy, solvent-free and ecofriendly method. Thus, this work contributes significantly to the search for new ecologically correct strategies for the control of T. castaneum.