DEFAR - Departamento de Farmácia
URI permanente desta comunidadehttp://www.hml.repositorio.ufop.br/handle/123456789/530
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Resultados da Pesquisa
Item Effects of Coffea arabica aqueous extracts and their main chemical constituents on hyperuricemia.(2022) Coelho, Grazielle Brandão; Bernardes, Ana Catharina Fernandes Pereira Ferreira; Müller, Camila Martins de Sá; Serra, Vanessa de Assis Barros; Guimarães, Dênia Antunes SaúdeAqueous extracts of Coffea arabica L., Rubiaceae, were prepared from green coffee beans, roasted coffee in three different conditions, and decaffeinated coffee. Caffeine, trigonelline, neochlorogenic, and chlorogenic acids were identified by HPLC/ DAD as the main chemical constituents in aqueous extracts, except for decaffeinated coffee. Effects of the extracts and substances on hyperuricemia were evaluated using the animal model of potassium oxonate-induced hyperuricemia. All extracts showed anti- hyperuricemic activity at all evaluated doses and were able to inhibit hepatic xanthine oxidase. Light roast beans’ aqueous extract (75 mg/kg) lowered serum uric acid (1.681 mg/dl) to levels comparable to the normouricemic control (1.628 mg/dl), and decaf- feinated coffee aqueous extract (75 mg/kg) displayed the highest percentage of liver xanthine oxidase inhibition (45.92%). The uricosuric effect of the extracts was observed in at least one evaluated dose. Light roast beans’ aqueous extract (225 mg/kg) promoted highest renal excretion of uric acid (32.97 mg/kg/5 h). All compounds detected presented hypouricemic effect, acting by uricostatic route. Chlorogenic and neochlorogenic acids demonstrated uricosuric, and uricostatic effects. These results support that Coffea arabic aqueous extracts and neochlorogenic and chlorogenic acids, trigonelline, and caffeine are promising agents for use in the treatment of hyperuricemia and gout.Item In vivo anti-hyperuricemic activity of sesquiterpene lactones from Lychnophora species.(2019) Bernardes, Ana Catharina Fernandes Pereira Ferreira; Coelho, Grazielle Brandão; Araújo, Marcela Carolina de Paula Michel; Guimarães, Dênia Antunes SaúdeHyperuricemia is the main cause of gout, an inflammation induced by uric acid deposition in joints. Drugs available present side effects, so there is a need for new treatment alternatives. Lychnophora species are used in folk medicine to treat inflammation, rheumatism and muscle pain. Goyazensolide (10 mg/kg), eremantholide C (25 mg/kg) and lychnopholide (25 mg/kg), sesquiterpene lactones isolated from Lychnophora species were previously studied and showed anti-hyperuricemic effects in mice. However, the mechanisms of this effect were not elucidated. The methodology of this study consisted in treatment of hyperuricemic-induced rats, and comparison between control groups, clinically used anti-hyperuricemic drugs and sesquiterpene lactones. Urine and blood were collected for uric acid quantification. Xanthine oxidase inhibition was measured in liver homogenates. Results showed that all evaluated sesquiterpene lactones presented anti-hyperuricemic activity at the doses of 5 and 10 mg/kg and can act through one or both mechanisms, depending on the dose administrated. Goyazensolide and lychnopholide at dose of 5 mg/kg showed important uricosuric effect. Goyazensolide and lychnopholide at dose of 10 mg/kg, and eremantholide C (5 and 10 mg/kg) presented notable inhibition of hepatic xanthine oxidase activity and uricosuric effect. Thus, these sesquiterpene lactones are promising hypouricemic agent to treat hyperuricemia and gout.