EFAR - Escola de Farmácia
URI permanente desta comunidadehttp://www.hml.repositorio.ufop.br/handle/123456789/451
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O curso de Farmácia em Ouro Preto foi criado em 1839, sendo a mais antiga Escola de Farmácia da América Latina.
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Item Experimental Chagas' disease in dogs.(1992) Lana, Marta de; Chiari, Egler; Tafuri, Washington LuizItem Hemocultures for the parasitological diagnosis of human chronic Chagas' disease.(1989) Chiari, Egler; Dias, João Carlos Pinto; Lana, Marta de; Chiari, Cléa de AndradeWith the purpose of standardization of an hemoculture technique presenting a higher positive rate in the parasitological diagnosis of chronic Chagas’ disease in patients with reactive serology (IFT, HA, CFT) the following schedule was used. Thirty ml of venous blood was collected with heparin and the plasma was separated by centrifugation (2.000 rpm/30'). The packed cells were washed with LIT medium or PBS which was then removed by centrifugation (2.000 rpm/15’). This material was sampled in 6 screw-tubes 18x200 with 6 ml of LIT medium and incubated at 28°C. These incubated cultures at 28°C were examined after 15, 30, 45 and 60 days. When the hemoculture was not immediately processed after blood collection, the plasma was removed and the sediment enriched with LIT medium and preserved at 4°C. The Xenodiagnosis was performed according to Schenones method used here as a reference technique. Among the various groups of patients examined by both techniques the best results obtained were: 55.08% ofpositivity for hemocultures against 27.5% forxenodiagnosis (X^ = 4.54, p = 0.05), with a tubepositivity of 26.6%. Recommendation for screening trials of drug assays is the repetition of method on a same patient 2 or more times in different occasions, as used in xenodiagnosis.Item Trypanosoma cruzi : effect of benznidazole therapy combined with the iron chelator desferrioxamine in infected mice.(2008) Francisco, Amanda Fortes; Vieira, Paula Melo de Abreu; Arantes, Jerusa Marilda; Pedrosa, Maria Lúcia; Martins, Helen Rodrigues; Silva, Maísa; Veloso, Vanja Maria; Lana, Marta de; Bahia, Maria Terezinha; Tafuri, Washington Luiz; Carneiro, Cláudia MartinsIron chelators have been employed in various studies aimed at evaluating the relationship between the iron status of the host and the development of infection. In the present study, the effects of benznidazole (BZ) therapy in combination with the iron chelator desferrioxamine (DFO) on the development of infection in mice inoculated with Trypanosoma cruzi Y strain have been investigated. Infected mice treated with DFO presented lower levels of parasitemia compared with infected untreated animals. Therapy with BZ for 21 days, with or without DFO, led to decreased parasitemia and reduced mortality, but BZ in combination with DFO treatment for 35 days (BZ/DFO-35) gave 0% mortality. All infected groups presented lower levels of iron in the liver, but serum iron concentrations were greater in DFO-35 and BZ/DFO-35, whereas hemoglobin levels were higher in BZ/DFO-35 and lower in DFO-35 compared with other treated groups. The percentage cure, determined from negative hemoculture and PCR results in animals that had survived for 60 days post-infection, was 18% for BZ and BZ/DFO-35, 42% for BZ combined with DFO for 21 days, and 67% for DFO-35. The results demonstrate that modification in iron stores increases BZ efficacy.Item Trypanosoma cruzi : immunoglobulin isotype profiles during the acute phase of canine experimental infection with metacyclic or blood trypomastigotes.(2008) Vital, Wendel Coura; Carneiro, Cláudia Martins; Martins, Helen Rodrigues; Lana, Marta de; Veloso, Vanja Maria; Carvalho, Andréa Teixeira de; Bahia, Maria Terezinha; Oliveira, Rodrigo Corrêa de; Martins Filho, Olindo Assis; Tafuri, Wagner Luiz; Reis, Alexandre BarbosaA detailed investigation has been carried out about the serological profiles of groups of dogs experimen-tally infected with metacyclic (MT) or blood (BT) trypomastigotes of Berenice-78 Trypanosoma cruzi strain. Peripheral blood was collected from infected dogs and uninfected controls, weekly during 35 days following the acute phase of infection, and immunoglobulin profiles were determined by ELISA. Dogs infected with BT exhibited unaltered levels of IgG2, increases in IgM, IgE, IgA, IgG and IgG1. In contrast, dogs infected with MT presented unaltered levels of IgE and IgG1 and an increase in IgM, IgA, IgG and IgG2 levels. Compared with the MT group, animals infected with BT showed significant increases in IgM on days 7, 14 and 28, in IgA on days 7, 14 and 21, in IgE on days 7 and 14, in IgG on days 14 and 28, and in IgG1 on days 7, 14 and 21. Parasitemia levels of the infected animals were measured over the same time period. No correlations were found between the immunoglobulin profiles and the parasi-temia levels. The results demonstrated that the inoculum source (BT or MT) influence the immunoglob-ulin isotype profile that may drive distinct outcome of acute canine Chagas diseaseItem Trypanosoma cruzi : serum levels of nitric oxide and expression of inducible nitric oxide synthase in myocardium and spleen of dogs in the acute stage of infection with metacyclic or blood trypomastigotes.(2009) Vieira, Paula Melo de Abreu; Francisco, Amanda Fortes; Souza, Sheler Martins de; Malaquias, Luiz Cosme Cotta; Reis, Alexandre Barbosa; Giunchetti, Rodolfo Cordeiro; Veloso, Vanja Maria; Lana, Marta de; Tafuri, Wagner Luiz; Carneiro, Cláudia MartinsThe par tic i pa tion of nitric oxide (NO) in the con trol of blood par a si te mia and par a sit ism dur ing the acute phase of infec tion in dogs inoc u lated with blood try pom astig otes ( BT) or meta cy clic try pom astig otes (MT group) of Bere nice -78 Try pan o soma cru zi strain has been eval u ated. Ani mals of the MT group (n = 4) pre sented increased lev els of serum NO through out the infec tion when com pared with the BT ( n = 4) or con trol (n = 4) groups, and a delay in par a si te mia peak com pared with the BT group. In spleen frag ments, tis sue par a sit ism was not observed but the MT group pre sented larger areas asso ci ated with induc ible NO syn thase (iNOS) in rela tion to BT and con trol groups. Heart frag ments of MT-infected ani mals exhib ited com par a tively low tis sue par a sit ism and high iNOS expres sion, while ani mals of the BT group pre sented high inflam ma tory infil trate, high tis sue par a sit ism and low iNOS expres sion. These results indi cate that the source of inoc u lum can inter- fere with the devel op ment of the acute phase of Cha gas dis ease, and may also trig ger a dis tinct par a site–host inter ac tion dur ing this phase.