EFAR - Escola de Farmácia
URI permanente desta comunidadehttp://www.hml.repositorio.ufop.br/handle/123456789/451
Notícias
O curso de Farmácia em Ouro Preto foi criado em 1839, sendo a mais antiga Escola de Farmácia da América Latina.
Navegar
80 resultados
Resultados da Pesquisa
Item Avaliação dos serviços de saúde e dos recursos materiais para rastreio do câncer cervical em Ouro Preto - MG.(2022) Oliveira, Renata Rocha e Rezende; Rezende, Giselle Aparecida de Souza; Silva, Bruna Viana; Lage, Ana Luiza; Martins, Bruna Aparecida; Veloso, Marco Antônio; Geöcze, Bruna Albuquerque; Abreu, Ronan David Souza; Rezende, Mariana Trevisan; Carneiro, Cláudia MartinsA realização periódica do exame de Papanicolaou é o método convencional no Brasil para o rastreio do Câncer do Colo do Útero (CCU). As estratégias de serviço e os recursos materiais nas Unidades Básicas de Saúde (UBS) são aspectos fundamentais que refletem na qualidade do programa de rastreamento. Objetivo: Analisar quatro UBS do município de Ouro Preto, Minas Gerais e verificar o contexto local de rastreio do CCU. Métodos: Foi realizado estudo qualitativo, baseado em entrevistas semiestruturadas com enfermeiros e agentes comunitários de saúde (ACS) das UBS selecionadas. Resultados: Observou-se que apenas uma UBS apresentava planta física e recursos materiais adequados à realização do exame. Em geral, as ACS realizavam busca ativa não sistematizada das pacientes para realização do exame de Papanicolau. A conduta para informar os resultados alterados às pacientes era semelhante entre as UBS, porém, não havia padronização para realizar o seguimento dos casos alterados. Além disso, as atividades educativas eram escassas para a comunidade e para a equipe de trabalho. Conclusão: Percebe-se que uma estrutura física de qualidade associada a uma equipe de saúde da família (ESF) treinada favorece o estabelecimento de programas de rastreio exitosos. Por não se tratar de uma realidade de todas as UBS locais, é necessário lançar princípios de organização para sistematizar as estratégias de busca ativa, registro e acompanhamento das pacientes, sendo os ACS fundamentais neste processo, devido à proximidade com a população.Item Emotionally subjective reactivity to cervical cytology pictures is modulated by expertise.(2022) Oliveira, Jacqueline Alfenas de; Souza, Miriam de Cássia; Cunha, Laila Fernandes da; Mota, Bruna Eugênia Ferreira; Rezende, Mariana Trevisan; Carneiro, Cláudia Martins; Pereira, Mirtes Garcia; Mocaiber, Izabela; Souza, Gabriela Guerra Leal deOur aims were to create a catalog of cytological pictures and to evaluate the valence (level of pleasantness/ unpleasantness) and arousal (level of calm/excitement) of these pictures in individuals with different occupations. The sample consisted of medical and law college students and cytopathologists. Valence and arousal score for general pictures were not modulated by expertise in cytology. However, students judged the cytological pictures to be lower in valence and in arousal than the cytopathologists. The cytopathologists classified cytological pictures with lesions as lower in valence and higher in arousal than cytological pictures without lesions.Item Serum biomarkers in patients with unilateral or bilateral active pulmonary tuberculosis : immunological networks and promising diagnostic applications.(2023) Pascoal, Vanessa Peruhype Magalhães; Araújo, Fernanda Fortes de; Papini, Tatiane Figueiredo de Morais; Wendling, Ana Paula Barbosa; Azevedo, Ana Carolina Campi; Reis, Jordana Grazziela Alves Coelho dos; Almeida, Isabela Neves de; Antonnelli, Lis Ribeiro do Valle; Amaral, Laurence Rodrigues do; Gomes, Matheus de Souza; Sousa, Joaquim Pedro Brito de; Santos, Silvana Maria Elói; Augusto, Valéria Maria; Dalcolmo, Margareth Maria Pretti; Carneiro, Cláudia Martins; Carvalho, Andréa Teixeira de; Martins Filho, Olindo AssisThe present observational study was designed to characterize the integrative profile of serum soluble mediators to describe the immunological networks associated with clinical findings and identify putative biomarkers for diagnosis and prognosis of active tuberculosis. The study population comprises 163 volunteers, including 84 patients with active pulmonary tuberculosis/(TB), and 79 controls/(C). Soluble mediators were measured by multiplexed assay. Data analysis demonstrated that the levels of CCL3, CCL5, CXCL10, IL-1β, IL-6, IFN-γ, IL-1Ra, IL-4, IL-10, PDGF, VEGF, G-CSF, IL-7 were increased in TB as compared to C. Patients with bilateral pulmonary involvement/(TB-BI) exhibited higher levels of CXCL8, IL-6 and TNF with distinct biomarker signatures (CCL11, CCL2, TNF and IL-10) as compared to patients with unilateral infiltrates/(TB-UNI). Analysis of biomarker networks based in correlation power graph demonstrated small number of strong connections in TB and TB-BI. The search for biomarkers with relevant implications to understand the pathogenetic mechanisms and useful as complementary diagnosis tool of active TB pointed out the excellent performance of single analysis of IL-6 or CXCL10 and the stepwise combination of IL-6 → CXCL10 (Accuracy = 84 %; 80 % and 88 %, respectively). Together, our finding demonstrated that immunological networks of serum soluble biomarkers in TB patients differ according to the unilateral or bilateral pulmonary involvement and may have relevant implications to understand the pathogenetic mechanisms involved in the clinical outcome of Mtb infection.Item Defining the early stages of intestinal colonisation by whipworms.(2022) Correa, Maria Adelaida Duque; Goulding, David; Rodgers, Faye H.; Gillis, J. Andrew; Cormie, Claire; Rawlinson, Kate A.; Bancroft, Allison J.; Bennett, Hayley M.; Lotkowska, Magda E.; Reid, Adam J.; Speak, Anneliese O.; Scott, Paul; Redshaw, Nicholas; Tolley, Charlotte; McCarthy, Catherine; Brandt, Cordelia; Sharpe, Catherine; Ridley, Caroline; Moya, Judit Gali; Carneiro, Cláudia Martins; Starborg, Tobias; Hayes, Kelly S.; Holroyd, Nancy; Sanders, Mandy; Thornton, David J.; Grencis, Richard K.; Berriman, MatthewWhipworms are large metazoan parasites that inhabit multi-intracellular epithelial tunnels in the large intestine of their hosts, causing chronic disease in humans and other mammals. How first-stage larvae invade host epithelia and establish infection remains unclear. Here we investigate early infection events using both Trichuris muris infections of mice and murine caecaloids, the first in-vitro system for whipworm infection and organoid model for live helminths. We show that larvae degrade mucus layers to access epithelial cells. In early syncytial tunnels, larvae are completely intracellular, woven through multiple live dividing cells. Using single-cell RNA sequencing of infected mouse caecum, we reveal that progression of infection results in cell damage and an expansion of enterocytes expressing of Isg15, potentially instigating the host immune response to the whipworm and tissue repair. Our results unravel intestinal epithelium invasion by whipworms and reveal specific host-parasite interactions that allow the whipworm to establish its multi-intracellular niche.Item Pharmacometrics : the already‑present future of precision pharmacology.(2022) Bandeira, Lorena Cera; Pinto, Leonardo; Carneiro, Cláudia MartinsThe use of mathematical modeling to represent, analyze, make predictions or providing information on data obtained in drug research and development has made pharmacometrics an area of great prominence and importance. The main purpose of pharmacometrics is to provide information relevant to the search for efcacy and safety improvements in pharmacotherapy. Regulatory agencies have adopted pharmacometrics analysis to justify their regulatory decisions, making those decisions more efcient. Demand for specialists trained in the feld is therefore growing. In this review, we describe the meaning, history, and development of pharmacometrics, analyzing the challenges faced in the training of professionals. Examples of applications in current use, perspectives for the future, and the importance of pharmacometrics for the development and growth of precision pharmacology are also presented.Item ASP-2/Trans-sialidase chimeric protein induces robust protective immunity in experimental models of chagas’ disease.(2023) Castro, Júlia Teixeira de; Brito, Rory Cristiane Fortes de; Souza, Natália Satchiko Hojo de; Azevedo, Bárbara Ribeiro Batista Vaz de; Castro, Natália Salazar de; Ferreira, Camila Pontes; Giusta, Caroline Junqueira; Fernandes, Ana Paula Salles Moura; Vasconcellos, José Ronnie Carvalho de; Cardoso, Jamille Mirelle de Oliveira; Soares, Rodrigo Dian de Oliveira Aguiar; Vieira, Paula Melo de Abreu; Carneiro, Cláudia Martins; Valiate, Bruno Vinícius Santos; Toledo, Cristiane; Salazar, Andres M.; Caballero, Otávia; Vieira, Joseli Lannes; Teixeira, Santuza Maria Ribeiro; Reis, Alexandre Barbosa; Gazzinelli, Ricardo TostesImmunization with the Amastigote Surface Protein-2 (ASP-2) and Trans-sialidase (TS) antigens either in the form of recombinant protein, encoded in plasmids or human adenovirus 5 (hAd5) confers robust protection against various lineages of Trypanosoma cruzi. Herein we generated a chimeric protein containing the most immunogenic regions for T and B cells from TS and ASP-2 (TRASP) and evaluated its immunogenicity in comparison with our standard protocol of heterologous prime-boost using plasmids and hAd5. Mice immunized with TRASP protein associated to Poly-ICLC (Hiltonol) were highly resistant to challenge with T. cruzi, showing a large decrease in tissue parasitism, parasitemia and no lethality. This protection lasted for at least 3 months after the last boost of immunization, being equivalent to the protection induced by DNA/hAd5 protocol. TRASP induced high levels of T. cruzispecific antibodies and IFNγ-producing T cells and protection was primarily mediated by CD8+ T cells and IFN-γ. We also evaluated the toxicity, immunogenicity, and efficacy of TRASP and DNA/hAd5 formulations in dogs. Mild collateral effects were detected at the site of vaccine inoculation. While the chimeric protein associated with Poly-ICLC induced high levels of antibodies and CD4+ T cell responses, the DNA/hAd5 induced no antibodies, but a strong CD8+ T cell response. Immunization with either vaccine protected dogs against challenge with T. cruzi. Despite the similar efficacy, we conclude that moving ahead with TRASP together with Hiltonol is advantageous over the DNA/hAd5 vaccine due to pre-existing immunity to the adenovirus vector, as well as the cost-benefit for development and large-scale production.Item CD4+ T-lymphocytes from asymptomatic dogs infected with Leishmania infantum are able to activate macrophages for higher leishmanicidal ability in an in vitro co-culture experiment.(2022) Vieira, João Filipe Pereira; Cardoso, Jamille Mirelle de Oliveira; Brito, Rory Cristiane Fortes de; Roatt, Bruno Mendes; Carneiro, Cláudia Martins; Valadares, Diogo Garcia; Soares, Rodrigo Dian de Oliveira Aguiar; Reis, Alexandre BarbosaDogs are the most common domestic reservoir of Leishmania infantum, making canine visceral leishmaniasis (CVL) a serious public health issue. Identifying new methodologies that can mimic lymphoid and myeloid competence in naturally infected dogs could lower costs and save time in preliminary screenings of potential immunotherapeutic agents and vaccines against CVL. For that, we established a cell-to-cell communication approach between lymphocytes and myeloid cells from healthy, asymptomatic (infected, without apparent clinical signs) and symptomatic (infected with apparent clinical signs) dogs. Peripheral blood mononuclear cells (PBMC) from these dogs were used as source of CD4+, CD8+ T lymphocytes and macrophages, that were posteriorly infected with L. infantum GFP+ promastigotes (green fluorescent protein). Macrophages co-cultured with purified lymphocytes were tested for the ability to control cellular parasitism, and their microbicidal function by producing nitric oxide (NO) and reactive oxygen species (ROS). The kind of T cell response within the co-culture was also evaluated, by assessing their ability to produce interferon-gamma (IFN-γ) and interleukin 4 (IL-4). The data suggests that T lymphocytes from symptomatic dogs are more prone to produce IL-4 than the ones from asymptomatic dogs. Macrophages from asymptomatic dogs also demonstrated a higher microbicidal potential, with increased levels of NO and ROS production, compared to symptomatic dogs, mainly in highly parasitized cells. Together, our results identify the ratio of IL-4/IFN-γ produced by CD4+ and CD8+ T cells, as well as, the ratio between parasite GFP signal/NO and ROS signal in macrophages as potential immunological biomarkers of failure and success of the screened agents. Our findings also propose a reliable methodology that can be used to follow the immune response in trials of potential drugs or vaccines targeting CVL.Item Performance of the Wondfo 2019-nCoV antigen test using self-collected nasal versus professional-collected nasopharyngeal swabs in symptomatic SARS-CoV-2 infection.(2022) Cardoso, Jamille Mirelle de Oliveira; Roatt, Bruno Mendes; Vieira, Paula Melo de Abreu; Paiva, Nívia Carolina Nogueira de; Souza, Breno Bernardes; Lisboa, Oscar Campos; Soares, Rodrigo Dian de Oliveira Aguiar; Reis, Alexandre Barbosa; Vital, Wendel Coura; Carneiro, Cláudia MartinsItem Cell immune response in mice skin stimulated with different adjuvants by intradermal route.(2022) Mathias, Fernando Augusto Siqueira; Cardoso, Jamille Mirelle de Oliveira; Reis, Levi Eduardo Soares; Souza, Juliana Vitoriano de; Moreira, Nádia das Dores; Ostolin, Thais Lopes Valentim Di Paschoale; Brito, Rory Cristiane Fortes de; Soares, Rodrigo Dian de Oliveira Aguiar; Vieira, Paula Melo de Abreu; Carneiro, Cláudia Martins; Roatt, Bruno Mendes; Reis, Alexandre BarbosaAdjuvants act in the innate immunity and, when combined to vaccine antigens, can produce a more intense response, improving the antigen presentation, directing the immune system, excellent for new vaccine formulations. This study evaluated the use of the intradermal route and the immune response triggered by a single dose of the adjuvants Aluminum Hydroxide (Al(OH)3 ), Montanide Pet Gel A (MPGA), Glucopyranosyl Lipid A Stable Emulsion (GLA-SE), and Resiquimod (R-848) in the mice skin. As control mice received sterile saline. MPGA and GLA-SE led to cell recruitment when compared with control group, with intense presence of neutrophils in first 12 hours, replaced by macrophages after 168 hours. R-848 and Al(OH)3 showed similar cell recruitment profiles. Regarding cytokine production, groups that received MPGA and GLA-SE produced high levels of IL-6, TNF-α, and IFN-γ. R-848 and Al(OH)3 groups displayed similar profile of cytokine production only at the first hour. Our results suggest that the intradermal route is efficient inducing immune system activation and GLA-SE was promising adjuvants for a type 1 immune response vaccine.Item Heterologous vaccine therapy associated with half course of Miltefosine promote activation of the proinflammatory response with control of splenic parasitism in a hamster model of visceral leishmaniasis.(2021) Carvalho, Lívia Mendes; Ferreira, Francielle Carvalho; Gusmão, Miriã Rodrigues; Costa, Ana Flávia Pereira; Brito, Rory Cristiane Fortes de; Soares, Rodrigo Dian de Oliveira Aguiar; Reis, Alexandre Barbosa; Cardoso, Jamille Mirelle de Oliveira; Carneiro, Cláudia Martins; Roatt, Bruno MendesVisceral leishmaniasis (VL) is a serious and neglected disease present worldwide. Chemotherapy using pentavalent antimony (SbV) is the most practical and inexpensive strategy available for the VL treatment today, however, it has high toxicity. Alternatively, other drugs are used as viable leishmanicidal therapeutic options. Miltefosine is the only anti-leishmanial agent administered orally, however, it has been reducing its effectiveness. In this sense, there is no ideal therapy for VL since the drugs currently used trigger severe side effects causing discontinuation of treatment, which carries an imminent risk for the emergence of parasite resistance. With that, other therapeutic strategies are gaining prominence. Among them, immunotherapy and/or immunochemotherapy, which the activation/modulation of the immune system can redirect the host’s immune response to an effective therapeutic result. Therefore, this work was designed to assess an immunochemotherapy protocol composed of half course of Miltefosine associated with LBSap vaccine (Milt+LBSap) using the hamster Mesocricetus auratus as an experimental model for VL treatment. When evaluating the main hematobiochemical, immunological and therapeutic efficacy parameters, it was demonstrated that the treatment with Milt+LBSap showed restoration of hematobiochemical condition and reduced serum levels of IgG-anti-Leishmania compared to animals infected non treated (INT). Beyond that, an increase in the number of CD4+ lymphocytes producers of IFN-γ in relation to INT or to animals treated with miltefosine during 28 days, and TNF-α increased compared to INT were observed. Also, it was found a reduction of IL-10-production in relation to INT, or animals that received LBSap vaccine only, or miltefosine, following by a reduction in the splenic parasitic burden. These results demonstrate that the immunochemotherapy protocol used can stimulate the immune response, inducing an expressive cellular response sufficient to control spleen parasitism, standing out as a promising proposal for the VL treatment.