DEMSC - Artigos publicados em periódicos
URI permanente para esta coleçãohttp://www.hml.repositorio.ufop.br/handle/123456789/8508
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Item Reversible parkinsonism after chronic cyclosporin treatment in renal transplantation.(2009) Viana, Bernardo de Mattos; Moura, André de Souza; Moreira, Janaina Matos; Cano Prais, Hugo Alejandro; Cardoso, Francisco Eduardo CostaItem First report of Ehrlichia ewingii detected by molecular investigation in dogs from Brazil.(2009) Oliveira, Luciana Souza de; Oliveira, Karla Andrade de; Pescatore, A. M.; Lamëgo, Márcia Rogéria de Almeida; Conceição, Lissandro Gonçalves; Galvão, Márcio Antônio Moreira; Siqueira, Cláudio Lísias Mafra deItem American cutaneous leishmaniasis in Southeast Brazil : space-time clustering.(1999) Coelho, George Luiz Lins Machado; Assunção, Renato; Mayrink, Wilson; Caiaffa, Waleska TeixeiraItem Trypanosoma cruzi recombinant complement regulatory protein : a novel antigen for use in an enzyme-linked immunosorbent assay for diagnosis of chagas’ disease.(2002) Meira, Wendell Sérgio Ferreira; Galvão, Lúcia Maria da Cunha; Gontijo, Eliane Dias; Coelho, George Luiz Lins Machado; Norris, Karen; Chiari, EglerCurrently, diagnosis of Chagas’ disease is based on serological methods, but due to the high occurrence of inconclusive results, more reliable methods are needed. The use of recombinant antigens for serodiagnosis of Chagas’ disease is recommended in order to increase the sensitivity and specificity of the serological tests. The Trypanosoma cruzi complement regulatory protein (CRP) is a surface glycoprotein present on the trypomastigote forms of the parasite, and the recombinant CRP (rCRP) was cloned in a mammalian expression system and purified by affinity chromatography. The purified recombinant protein was used as an antigen in an enzyme-linked immunosorbent assay (rCRP ELISA) in order to verify its sensitivity and specificity compared with other established methods. In this evaluation, a panel of 184 serum samples distributed among chronic chagasic patients (n 65), blood bank donors (n 100), and patients infected with Leishmania spp. (n 19) was used. The sensitivity and specificity of the rCRP ELISA were 100% when compared to conventional serology and complement-mediated lysis tests from these groups. When hemoculture and PCR tests were evaluated for diagnosis of chronic chagasic patients, using the rCRP ELISA as a reference test, the positivities were found to be 64.62 and 81.54%, respectively, showing a higher degree of sensitivity of the test. The data demonstrate that rCRP ELISA was able to discriminate between chronic chagasic patients and nonchagasic individuals, such as blood donors and patients with leishmaniasis. Thus, the rCRP is an excellent antigen for use in Chagas’ disease diagnosis, due to the absence of false-negative or false-positive results.Item Risk factors for mucosal manifestation of American cutaneous leishmaniasis.(2003) Coelho, George Luiz Lins Machado; Caiaffa, Waleska Teixeira; Genaro, Odair; Magalhães, Paulo Araújo; Mayrink, WilsonA case-comparison study was carried out to identify risk factors for mucosal manifestations of American cutaneous leishmaniasis (ACL) in southeast Brazil, using a series of 2820 patients, diagnosed with ACL between 1966 and 1999. The significant factors independently associated with mucosal leishmaniasis were: gender, age, nutritional status and length of disease. Mucosal leishmaniasis occurred 1.7 times more frequently among males than females; twice as often in individuals older than 22 years compared with the younger group; almost four times as often in individuals with severe malnutrition compared with those who were well nourished; and almost four times more frequently in individuals reporting the disease for more than 4 months compared with those reporting a shorter duration of the disease. Among individuals older than 22 years the risk of mucosal leishmaniasis increased significantly (from 1.9 to 9.6) as the nutritional status decreased, when compared with younger and well-nourished patients. The characteristics herein described and correlated with severe forms could be used as diagnostic markers as part of clinical screening in areas endemic for ACL.Item The influence of copper, selenium and zinc on the response to the Montenegro skin test in subjects vaccinated against American cutaneous leishmaniasis.(2007) Araújo, Alexandra Paiva; Rocha, Olguita Geralda Ferreira; Mayrink, Wilson; Coelho, George Luiz Lins MachadoWe evaluated the relationship between the trace elements copper, zinc and selenium and the response to the Montenegro skin test (MST) in 172 volunteers vaccinated against American cutaneous leishmaniasis. The MST diameter was categorized as negative and in quartiles of positive response, constituting five groups. Trace element serum levels were analyzed by coupled plasma atomic emission spectrometry and hydride generation atomic absorption spectrometry, with study subjects classified into two groups depending on low or high levels of trace elements observed. MST-positive subjects had an MST diameter (mean ± SD) of 10.35 ± 4.64 mm, with copper, selenium and zinc serum levels of 1433.7 ± 665.7 g/l, 88.6 ± 39g/l and 999.2 ± 366g/l, respectively. The MST diameter was significantly different in the selenium groups only. The selenium levels also differed with the quartiles of the MST diameters of individuals testing positive (P < 0.05). Our findings suggest that trace elements, particularly selenium, should be measured in future vaccine trials so that vaccine immunogenicity and response can be assessed and compared between different studies.Item Rickettsiosis of the genus rickettsia in South America.(2003) Galvão, Márcio Antônio Moreira; Mafra, Cláudio Lísias; Moron, Cecília; Anaya, Elisabeth; Walker, David HughesIn South America, human cases of infection by the genus Rickettsia have been described in several countries in the last twenty years. The role of international organizations, such as the Centers for Disease Control and Prevention in Atlanta, Georgia, USA and the World Health Organization Collaborating Center for Tropical Diseases at the University of Texas Medical Branch at Galveston, Texas, USA, was very important in the last twenty years for the development of surveillance systems and for the increase in notification of rickettsial diseases by the countries of South America. We hope that the next goal will be prevention and control of rickettsial diseases in the countries of South America, as well as maintaining the programs developed during the last twenty years, so that a good health system and improved social conditions will be possible.Item Prevalence of antibodies to spotted fever group rickettsiae in humans and domestic animals in a brazilian spotted fever−endemic area in the state of São Paulo, Brazil : serologic evidence for infection by Rickettsia rickettsii and another spotted fever group rickettsia.(2004) Horta, Maurício Cláudio; Labruna, Marcelo Bahia; Sangioni, Luis Antônio; Vianna, Manoella Campostrini Barreto; Gennari, Solange Maria; Galvão, Márcio Antônio Moreira; Mafra, Cláudio Lísias; Vidotto, Odilon; Schumaker, Teresinha T. S.; Walker, David HughesIn serum samples obtained from all the healthy humans, horses, dogs, and donkeys present on three farms in the Pedreira Municipality, an endemic area for Brazilian spotted fever, an indirect immunofluorescence assay (IFA) detected antibodies against Rickettsia rickettsii in 17 (77.3%) horses, 5 (31.3%) dogs (titers ranging from 64 to 4,048), and none of 4 donkeys or 50 humans. Five canine and eight equine sera with high antibody titers to R. rickettsii were also tested by IFA against R. bellii, R. akari, and R. africae antigens. Sera from two horses and two dogs that showed similar high antibody titers against two rickettsial antigens were evaluated after cross-absorption. Sera from seven horses and two dogs contained antibodies specific for R. rickettsii, and one dog serum had antibodies against a Rickettsia species very closely related to R. africae. The latter may have been caused by infection with the recently identified COOPERI strain.Item Revisiting Brazilian spotted fever focus of Caratinga, Minas Gerais state, Brazil.(2006) Galvão, Márcio Antônio Moreira; Cardoso, Luciane Daniele; Mafra, Cláudio Lísias; Calic, Simone Berger; Walker, David HughesWe revisited a Brazilian spotted fever focal area in Minas Gerais state, Brazil, in 2002, and performed a serologic survey in dogs and cats. The results of this survey are compared with the survey made 10 years before. The possible efficacy of vector control measures adopted in this area and the role of dogs and horses as sentinels of infection by Rickettsia are discussed.Item Rickettsia felis in the Americas.(2006) Galvão, Márcio Antônio Moreira; Zavala Velazquez, Jorge Ernesto; Calic, Simone Berger; Walker, David HughesThe authors describe their work in the Americas in Rickettsia felis cases in humans and the presence of Rickettsia felis in vectors.