DEMSC - Artigos publicados em periódicos

URI permanente para esta coleçãohttp://www.hml.repositorio.ufop.br/handle/123456789/8508

Navegar

Filtros

2006 - 200912010 - 20192

Configurações

Autor: search.filters.author.Coelho, George Luiz Lins MachadoAssunto: search.filters.subject.Diagnosis

Resultados da Pesquisa

Agora exibindo 1 - 3 de 3
  • Nenhuma Miniatura Disponível
    Item
    Evaluation of three recombinant proteins for the development of ELISA and immunochromatographic tests for visceral leishmaniasis serodiagnosis.
    (2019) Santos, Anna Raquel Ribeiro dos; Serufo, Ângela Vieira; Figueiredo, Maria Marta; Godoi, Lara Carvalho; Vitório, Jéssica Gardone; Marcelino, Andreza Pain; Avelar, Daniel Moreira de; Rodrigues, Fernandes Tenório Gomes; Coelho, George Luiz Lins Machado; Medeiros, Fernanda Alvarenga Cardoso; Jeronimo, Selma Maria Bezerra; Oliveira, Edward José de; Nascimento, Frederico Crepaldi; Teixeira, Santuza Maria Ribeiro; Gazzinelli, Ricardo Tostes; Nagem, Ronaldo Alves Pinto; Fernandes, Ana Paula Salles Moura
    BACKGROUND Visceral Leishmaniasis (VL) is an infectious disease that is a significant cause of death among infants aged under 1 year and the elderly in Brazil. Serodiagnosis is a mainstay of VL elimination programs; however, it has significant limitations due to low accuracy. OBJECTIVE This study aimed to evaluate three recombinant Leishmania infantum proteins (rFc, rC9, and rA2) selected from previous proteomics and genomics analyses to develop enzyme-linked immunosorbent assay (ELISA) and immunochromatographic tests (ICT) for the serodiagnosis of human VL (HVL) and canine VL (CVL). METHODS A total of 186 human (70 L. infantum-infected symptomatic, 20 other disease-infected, and 96 healthy) and 185 canine (82 L. infantum-infected symptomatic, 27 L. infantum-infected asymptomatic, and 76 healthy) sera samples were used for antibody detection. FINDINGS Of the three proteins, rA2 (91.5% sensitivity and 87% specificity) and rC9 (95.7% sensitivity and 87.5% specificity) displayed the best performance in ELISA-HVL and ELISA-CVL, respectively. ICT-rA2 also displayed the best performance for HVL diagnosis (92.3% sensitivity and 88.0% specificity) and had high concordance with immunofluorescence antibody tests (IFAT), ELISA-rK39, IT-LEISH®, and ELISAEXT. ICT-rFc, ICT-rC9, and ICT-rA2 had sensitivities of 88.6%, 86.5%, and 87.0%, respectively, with specificity values of 84.0%, 92.0%, and 100%, respectively for CVL diagnosis. MAIN CONCLUSIONS The three antigens selected by us are promising candidates for VL diagnosis regardless of the test format, although the antigen combinations and test parameters may warrant further optimisation.
  • Nenhuma Miniatura Disponível
    Item
    Comparison among three polymerase chain reaction assays on detection of DNA from Leishmania in biological samples from patients with american cutaneous leishmaniasis.
    (2012) Silva, João Guilherme Lino da; Silva, Thiago Miranda da; Peloso, Eduardo de Figueiredo; Coelho, George Luiz Lins Machado; Mayrink, Wilson; Ariosa, Marília Caixeta Franco; Silva, Paulo Márcio de Faria e; Marques, Marcos José
    Introdução: Analisou-se a positividade da reação em cadeia da polimerase (PCR) na detecção de DNA de Leishmania em pacientes. Métodos: DNA extraído foi submetido a L150/L152, 13Y/13Z e PCR seminested (snPCR). Resultados: Resultados foram evidenciados por bandas de aproximadamente 120; 720 e 670pb para L150/L152, 13Y/13Z e snPCR, respectivamente. Positividades para L150/L152, 13Y/13Z e snPCR foram 76,9; 56,4 e 69,2 (p > 0,05), para suspeitos; e 93,7; 68,7 e 84,4 (p < 0,05) para confirmados, respectivamente. Conclusões: Resultados preliminares mostraram que os ensaios, principalmente L150/L152 e snPCR, podem detectar DNA de Leishmania e têm potencial para diagnóstico laboratorial das leishmanioses.
  • Nenhuma Miniatura Disponível
    Item
    Comparison of polymerase chain reaction with other laboratory methods for the diagnosis of American cutaneous leishmaniasis Diagnosis of cutaneous leishmaniasis by polymerase chain reaction.
    (2006) Marques, Marcos José; Volpini, Ângela Cristina; Coelho, George Luiz Lins Machado; Pinto, Jackson Machado; Costa, Carlos Alberto da; Mayrink, Wilson; Genaro, Odair; Romanha, Alvaro José
    An evaluation of 5 laboratory methods for diagnosing American cutaneous leishmaniasis (ACL) was carried out on patients from an endemic area of Brazil. From 164 patients presenting cutaneous lesions, and suspected to have ACL, 133 (81.1%) were confirmed for the disease by Montenegro skin test (MST) and/or parasitologic examination (PE). In both groups of patients, the positivity of polymerase chain reaction (PCR) was similar to that of immunofluorescence assay and enzyme-linked immunosorbent assay, and higher than that of MST and PE ( P b .05). In the group of patients suspected to have ACL, PCR presented the same positivity as PE and MST together. No correlation between positivity of the laboratory methods and clinical or epidemiologic aspects was observed. Our data confirmed the value of PCR as an alternative laboratory method for diagnosing ACL, especially for those patients with negative PE and MST.