DEFAR - Artigos publicados em periódicos

URI permanente para esta coleçãohttp://www.hml.repositorio.ufop.br/handle/123456789/531

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20202

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Assunto: search.filters.subject.Next-generation sequencing

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    Deep sequencing of small RNAs reveals the repertoire of miRNAs and piRNAs in Biomphalaria glabrata.
    (2020) Queiroz, Fábio Ribeiro; Portilho, Laysa Gomes; Jeremias, Wander de Jesus; Babá, Élio Hideo; Amaral, Laurence Rodrigues do; Silva, Luciana Maria; Coelho, Paulo Marcos Zech; Caldeira, Roberta Lima; Gomes, Matheus de Souza
    BACKGROUND Biomphalaria glabrata snails are widely distributed in schistosomiasis endemic areas like America and Caribe, displaying high susceptibility to infection by Schistosoma mansoni. After the availability of B. glabrata genome and transcriptome data, studies focusing on genetic markers and small non-coding RNAs have become more relevant. The small RNAs have been considered important through their ability to finely regulate the gene expression in several organisms, thus controlling the functions like cell growth, metabolism, and susceptibility/resistance to infection. OBJECTIVE The present study aims on identification and characterisation of the repertoire of small non-coding RNAs in B. glabrata (Bgl-small RNAs). METHODS By using small RNA sequencing, bioinformatics tools and quantitative reverse transcription polymerase chain reaction (RT-qPCR), we identified, characterised, and validated the presence of small RNAs in B. glabrata. FINDINGS 89 mature miRNAs were identified and five of them were classified as Mollusk-specific. When compared to model organisms, sequences of B. glabrata miRNAs showed a high degree of conservation. In addition, several target genes were predicted for all the mature miRNAs identified. Furthermore, piRNAs were identified in the genome of B. glabrata for the first time. The B. glabrata piRNAs showed strong conservation of uridine as first nucleotide at 5’ end, besides adenine at 10th position. Our results showed that B. glabrata has diverse repertoire of circulating ncRNAs, several which might be involved in mollusk susceptibility to infection, due to their potential roles in the regulation of S. mansoni development. MAIN CONCLUSIONS Further studies are necessary in order to confirm the role of the Bgl-small RNAs in the parasite/host relationship thus opening new perspectives on interference of small RNAs in the organism development and susceptibility to infection.
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    Genetic evaluation of migratory fish : implications for conservation and stocking programs.
    (2020) Pimentel, Juliana da Silva Martins; Ludwig, Sandra; Resende, Leonardo Cardoso; Dias, Pedro Ferreira Pinto Brandão; Pereira, Adriana Heloísa; Abreu, Nazaré Lúcio de; Rosse, Izinara Cruz; Martins, Ana Paula Vimieiro; Facchin, Susanne; Lopes, João de Magalhães; Santos, Gilmar Bastos; Alves, Carlos Bernado Mascarenhas; Kalapothakis, Evanguedes
    Fish stocking programs have been implemented to mitigate the blockage of original riverbeds by the construction of hydropower dams, which affects the natural migra- tion of fish populations. However, this method raises concerns regarding the genetic rescue of the original populations of migratory fish species. We investigated the spa- tial distribution of genetic properties, such as genetic diversity, population structure, and gene flow (migration), of the Neotropical migratory fish Prochilodus costatus in the Três Marias dam in the São Francisco River basin, Brazil, and examined the possi- ble effects of fish stocking programs on P. costatus populations in this region. In total, 1,017 specimens were sampled from 12 natural sites and a fish stocking program, and genotyped for high-throughput sequencing at 8 microsatellite loci. The populations presented low genetic variability, with evidence of inbreeding and the presence of only four genetic pools; three pools were observed throughout the study region, and the fourth was exclusive to one area in the Paraopeba River. Additionally, we identi- fied high unidirectional gene flow between regions, and a preferred migratory route between the Pará River and the upper portion of the São Francisco River. The fish stocking program succeeded in transposing the genetic pools from downstream to upstream of the Três Marias dam, but, regrettably, promoted genetic homogenization in the upper São Francisco River basin. Moreover, the data show the fragility of this species at the genetic level. This monitoring strategy could be a model for the devel- opment of conservation and management measures for migratory fish populations that are consumed by humans.