DEFAR - Artigos publicados em periódicos
URI permanente para esta coleçãohttp://www.hml.repositorio.ufop.br/handle/123456789/531
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Item Angiotensin‐converting enzyme gene (ACE) polymorphisms are associated with dysregulation of biochemical parameters in hypertensive patients.(2023) Agostini, Lívia da Cunha; Cunha, Warlley Rosa; Silva, Nayara Nascimento Toledo; Melo, André Sacramento; Soares, Luciana Bicalho Moreira; Almeida, Tamires Cunha; Belo, Vanessa de Almeida; Vital, Wendel Coura; Teixeira, Luiz Fernando de Medeiros; Lima, Angélica Alves; Silva, Glenda Nicioli daIntroduction The genetic component, including genes and their variants, plays a signifcant role in the pathophysiology of arterial hypertension (AH). Thus, clinical, epidemiological and genetic studies have been carried out to improve the under- standing of disease mechanisms, improve diagnostic quality and contribute to prevention. Objective To determine the association of risk factors, biochemical parameters and diferent ACE gene polymorphisms with AH. Method The case-control study was carried out in the population of Ouro Preto, Brazil. The subjects answered a question- naire containing clinical and sociodemographic data. The ACE gene polymorphisms rs4291, rs4363 and rs4335 were evalu- ated by real time-polymerase chain reaction (real-time PCR) in 310 people (155 hypertensive and 155 normotensive patients), in addition to biochemical parameters. A multivariate logistic regression model was used to identify factors associated with AH. Analysis of continuous variables was performed using the Kruskal-Wallis test to assess signifcance between groups and Dunn’s post-test for multiple comparisons. Results The results showed that AH was associated with age, education, smoking, obesity and high levels of triglycerides, sodium, glucose and uric acid. Regarding the biochemical parameters, in hypertensive patients, the rs4363 and rs4335 poly- morphisms were associated with high levels of triglycerides, urea and glucose; the rs4291 polymorphism was associated with elevated urea and glucose levels. No association was detected between SNPs and HA. Conclusion AH was associated with socioeconomic status, lifestyle habits and biochemical parameters. ACE polymorphisms may have infuenced the levels of triglycerides, urea and glucose in hypertensive patients.Item Prevalence and factors associated with anxiety among university students of health sciences in Brazil : findings and implications.(2021) Alves, Júlia Vasconcelos de Sá; Paula, Waléria de; Netto, Patrícia Ribeiro Rezende; Godman, Brian; Nascimento, Renata Cristina Rezende Macedo do; Vital, Wendel CouraObjective: The aim was to evaluate the prevalence and factors associated with anxiety disorders among university students of health sciences at Federal University of Ouro Preto, Brazil. Methods: A cross-sectional study between March to June 2019. Data were collected through a self-administered questionnaire including sociodemographic, academic, family and behavioral issues. The Beck Anxiety Inventory was used to assess anxiety. Estimates were obtained through the prevalence ratio and Poisson multivariate analysis. Results: Four hundred and ninety-three students participated with a mean age of 23.1 and predominantly women (79.9%). All students had some degree of anxiety, with the frequency of the severe, moderate and mild forms being 28.0%, 29.8% and 27.0%, respectively. The factors associated with anxiety included having suffered psychological and/or physical violence in childhood, having suicidal thoughts, having a deceased parent, living with parents, being dissatisfied with the course and being in the exam period. Conclusions: The prevalence of anxiety was high in our study and family problems prior to entering university seem to significantly influence the degree of anxiety, which may compromise the student’s academic and social performance.Item A new Brazilian regional scenario of Type 2 diabetes risk in the next ten years.(2021) Nascimento, Lúbia Guaima; Nascimento, Renata Cristina Rezende Macedo do; Frade, Josélia Cintya Quintão Pena; Pinheiro, Eliete Bachrany; Ferreira, Wesley Magno; Reis, Janice Sepúlveda; Melo, Karla Fabiana Santana de; Pontarolo, Roberto; Lenzi, Mônica Soares Amaral; Almeida, José Vanilton de; João, Walter Jorge; Pedrosa, Hermelinda Cordeiro; Correr, Cassyano Januário; Vital, Wendel CouraAims: According to a recent national diabetes screening performed by our group in 2018, 18.4% of the Brazilians were found to have high blood glucose. The objective of the present study was to estimate the risk of developing type 2 DM (T2DM) in the next ten years in Brazilian population. Methods: A cross-sectional study was carried out in community pharmacies across Brazil, in 2018, where pharmacists applied the FINDRISC questionnaire to estimate the population’s risk of developing T2DM within a ten-year period. Results: The study included 977 pharmacists from 345 municipalities distributed across the five geograph- ical regions of Brazil. Of the 17,580 people evaluated, the South region was found to have the highest frequency (59.6%) among people at very low and/or low risk of developing T2DM, while the North region, the most underserved, presented the highest and/or very highest T2DM risk (24.1%). The factors that mostly and importantly impacted these regional differences were body mass index; the highest daily consumption of vegetables and fruits; history of high blood glucose and family history of T1DM/T2DM. Conclusion: These results showed an impressive change of direction concerning diabetes numbers between the most underserved region in public health care and one of the most developed and best organized regions concerning health assistance, the North and the South, respectively.Item Prevalence and factors associated with anxiety among university students of health sciences in Brazil : findings and implications.(2021) Alves, Júlia Vasconcelos de Sá; Paula, Waléria de; Netto, Patrícia Ribeiro Rezende; Godman, Brian; Nascimento, Renata Cristina Rezende Macedo do; Vital, Wendel CouraObjective: The aim was to evaluate the prevalence and factors associated with anxiety disorders among university students of health sciences at Federal University of Ouro Preto, Brazil. Methods: A cross-sectional study between March to June 2019. Data were collected through a self-administered questionnaire including sociodemographic, academic, family and behavioral issues. The Beck Anxiety Inventory was used to assess anxiety. Estimates were obtained through the prevalence ratio and Poisson multivariate analysis. Results: Four hundred and ninety-three students participated with a mean age of 23.1 and predominantly women (79.9%). All students had some degree of anxiety, with the frequency of the severe, moderate and mild forms being 28.0%, 29.8% and 27.0%, respectively. The factors associated with anxiety included having suffered psychological and/or physical violence in childhood, having suicidal thoughts, having a deceased parent, living with parents, being dissatisfied with the course and being in the exam period. Conclusions: The prevalence of anxiety was high in our study and family problems prior to entering university seem to significantly influence the degree of anxiety, which may compromise the student’s academic and social performance.Item Immunotherapy and immunochemotherapy in visceral leishmaniasis : promising treatments for this neglected disease.(2014) Roatt, Bruno Mendes; Soares, Rodrigo Dian de Oliveira Aguiar; Vital, Wendel Coura; Ker, Henrique Gama; Moreira, Nádia das Dores; Souza, Juliana Vitoriano de; Giunchetti, Rodolfo Cordeiro; Carneiro, Cláudia Martins; Reis, Alexandre BarbosaLeishmaniasis has several clinical forms: self-healing or chronic cutaneous leishmaniasis or post-kala-azar dermal leishmaniasis; mucosal leishmaniasis; visceral leishmaniasis (VL), which is fatal if left untreated.The epidemiology and clinical features of VL vary greatly due to the interaction of multiple factors including parasite strains, vectors, host genetics, and the environment. Human immunodeficiency virus infection augments the severity of VL increasing the risk of developing active disease by 100–2320 times. An effective vaccine for humans is not yet available. Resistance to chemotherapy is a growing problem in many regions, and the costs associated with drug identification and development, make commercial production for leishmaniasis, unattractive.The toxicity of currently drugs, their long treatment course, and limited efficacy are significant concerns. For cutaneous disease, many studies have shown promising results with immunotherapy/immunochemotherapy, aimed to modulate and activate the immune response to obtain a therapeutic cure. Nowadays, the focus of many groups centers on treating canine VL by using vaccines and immunomodulators with or without chemotherapy. In human disease, the use of cytokines like interferon-g associated with pentavalent antimonials demonstrated promising results in patients that did not respond to conventional treatment. In mice, immunomodulation based on monoclonal antibodies to remove endogenous immunosuppressive cytokines (interleukin-10) or block their receptors, antigen-pulsed syngeneic dendritic cells, or biological products like Pam3Cys (TLR ligand) has already been shown as a prospective treatment of the disease. This review addresses VL treatment, particularly immunotherapy and/or immunochemotherapy as an alternative to conventional drug treatment in experimental models, canine VL, and human disease.Item Evaluation of a prototype flow cytometry test for serodiagnosis of canine visceral leishmaniasis.(2013) Ker, Henrique Gama; Vital, Wendel Coura; Soares, Rodrigo Dian de Oliveira Aguiar; Roatt, Bruno Mendes; Moreira, Nádia das Dores; Carneiro, Cláudia Martins; Machado, Evandro Marques de Menezes; Carvalho, Andréa Teixeira de; Martins Filho, Olindo Assis; Giunchetti, Rodolfo Cordeiro; Araújo, Márcio Sobreira Silva; Coelho, Eduardo Antônio Ferraz; Lemos, Denise da Silveira; Reis, Alexandre BarbosaCanine visceral leishmaniasis (CVL) is considered one of the most important canine protozoan diseases of zoonotic concern (1). Various species of Phlebotomus and Lutzomyia sandflies are the potential vectors for the pathogenic agent Leishmania infantum (2). In some European, Asian, and African countries and in America, infection in dogs is associated with a risk of human disease (3–5). In Brazil, the Ministry of Health, through the Visceral Leishmaniasis Control and Surveillance Program (VLCSP), has instituted specific measures to reduce morbidity and case fatality rates, including treating human cases, instituting vector control, and, an action that is unique in the world, sacrificing all seropositive/infected dogs and prohibiting the treatment of CVL (6). During the last decade, the criteria for eliminating infected animals were based on enzyme-linked immunosorbent assays (ELISAs) for screening and indirect immunofluorescence antibody tests (IFATs) for the confirmatory diagnosis of CVL (6, 7). That these tests may lead to false-positive results due to crossreactivity with other parasitic diseases is well known (8, 9). Recently, this approach was modified, and testing is now based on a dual-path platform (DPP) for screening and an ELISA for confirmation (10). However, Grimaldi et al. (11) evaluated the DPP test for the serodiagnosis of CVL and showed that it does not perform well in detecting asymptomatic dogs from areas where canine disease is endemic. It has been shown that vaccination with Leishmune may lead to seroconversion in healthy dogs (10). The vaccination of dogs has increasingly become a common practice in areas in Brazil where CVL is endemic; recently, in addition to the Leishmune vaccine, the Leish-Tec vaccine has become available commercially, and new candidates, such as the LBSap vaccine, are being studied (12– 15). In this sense, seroconversion has become an important problem for surveillance/control programs that employ conventional methodologies in their seroepidemiological surveys, because it can lead to the unnecessary euthanasia of healthy dogs. Nevertheless, the role of vaccination in the diagnosis of CVL still has not been studied sufficiently.Item A multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis.(2014) Fraga, Deborah Bittencourt Mothé; Silva, Edimilson Domingos da; Pacheco, Luciano Vasconcellos; Borja, Lairton Souza; Oliveira, Isaac Queiroz de; Vital, Wendel Coura; Monteiro, Glória Regina; Oliveira, Geraldo Gileno de Sá; Jerônimo, Selma Maria Bezerra; Reis, Alexandre Barbosa; Veras, Patrícia Sampaio TavaresBackground: Visceral leishmaniasis (VL) is a serious public health challenge in Brazil and dogs are considered to be the main urban reservoir of the causative agent. The culling of animals to control VL in some countries makes the accurate diagnosis of canine VL (CVL) essential. Recombinant antigens rLci1A and rLci2B were selected from a cDNA library of Leishmania infantum amastigotes due to their strong potential as candidates in diagnostic testing for CVL. The present multicentric study aimed to evaluate the sensitivity of a prototype test using these antigens (DPPrLci1A/rLci2B) against 154 sera obtained from symptomatic dogs within three endemic areas of VL in Brazil. The specificity was evaluated using 40 serum samples from negative dogs and dogs infected with other pathogens. Sensitivity and specificity rates of DPP rLci1A/rLci2B prototype were compared to rates from other diagnostic tests currently in use by the Brazilian Ministry of Health, including DPP®LVC, EIE®LVC. Findings: DPP rLci1A/rLci2B prototype offered similar performance to that offered by DPP®LVC rapid test, as follows: sensitivity of 87% (CI 81–91) and 88% (CI 82–93) and specificity of 100% (CI 91–100) and 97% (CI 87–100), respectively for DPP rLci1A/rLci2B and DPP®LVC. When results of these two tests were considered concomitantly, sensitivity increased to 93.5% (CI 89–96). Conclusions: The recombinant antigens rLci1A and rLci2B represent promising candidates for use in a multi-antigen rapid test for CVL. The inclusion of novel antigens to the DPP rLci1A/rLci2B prototype model could offer additionally enhanced sensitivity to detect animals infected by L. infantum.Item Relationship between the professional of reference and the permanence of ADHD patients in a psychosocial care center for children and adolescents in Minas Gerais.(2015) Guedes, Lucas Araujo; Vital, Wendel Coura; Magalhães, Christine Vianna Algarves; Serra, Carla Penido; Veloso, Vanja Maria