DEFAR - Artigos publicados em periódicos

URI permanente para esta coleçãohttp://www.hml.repositorio.ufop.br/handle/123456789/531

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2013 - 201962020 - 20223

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Autor: search.filters.author.Amparo, Tatiane Roquete

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Agora exibindo 1 - 9 de 9
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    Modulation of long non-coding RNAs by different classes of secondary metabolites from plants : a mini-review on antitumor effects.
    (2022) Almeida, Tamires Cunha; Seibert, Janaína Brandão; Amparo, Tatiane Roquete; Souza, Gustavo Henrique Bianco de; Silva, Glenda Nicioli da; Santos, Orlando David Henrique dos
    The broad pharmacological spectrum of plants is related to their secondary metabolism, which is responsible for the synthesis of different compounds that have multiple effects on cellular physiology. Among the biological effects presented by phytochemicals, their use for the prevention and treatment of cancer can be highlighted. This occurs due to several mechanisms of antitumor ac- tion demonstrated by these compounds, including regulation of the cell signaling pathways and inhibi- tion of tumor growth. In this way, long non-coding RNAs (lncRNAs) appear to be promising targets for the treatment of cancer. Their deregulation has already been related to a variety of clinical- pathological parameters. However, the effects of secondary metabolites on lncRNAs are still restrict- ed. For this reason, the present review aimed to gather data on phytochemicals with action on lncRNAs in order to confirm their possible antitumor potential. According to the literature, terpenoid and flavonoid are the main examples of secondary metabolites involved with lncRNAs activity. In addition, the lncRNAs H19, CASC2, HOTAIR, NKILA, CCAT1, MALAT1, AFAP1-AS1, MEG3, and CDKN2B-AS1 can be highlighted as important targets in the search for new anti-tumor agents since they act as modulating pathways related to cell proliferation, cell cycle, apoptosis, cell migration and invasion. Finally, challenges for the use of natural products as a commercial drug were also discussed. The low yield, selectivity index and undesirable pharmacokinetic parameters were emphasized as a difficulty for obtaining these compounds on a large scale and for improving the potency of its biologi- cal effect. However, the synthesis and/or development of formulations were suggested as a possible approach to solve these problems. All of these data together confirm the potential of secondary me- tabolites as a source of new anti-tumor agents acting on lncRNAs.
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    Chemical constituents, antioxidant potential, antibacterial study and photoprotective activity of Brazilian corn silk extract.
    (2022) Azevedo, Amanda Scofield de; Seibert, Janaína Brandão; Amparo, Tatiane Roquete; Antunes, Amanda dos Santos; Sousa, Lucas Resende Dutra; Souza, Gustavo Henrique Bianco de; Teixeira, Luiz Fernando de Medeiros; Vieira, Paula Melo de Abreu; Santos, Viviane Martins Rebello dos; Nascimento, Adriana Mendes do; Nascimento, Andréa Mendes do
    The aim of this work was to determine the total phenolic content and evaluate the antioxidant, antibacterial, photoprotective and cytotoxic properties of corn silk ethanol extract prepared from corn silk that was air-dried at room temperature. Corn silk ethanol extract has a high content of phenolic compounds. The concentrations of ethanol extract required to produce 50% of maximal effect (EC50) in DPPH and ABTS radical scavenging assays were 489.0 and 166.1 μg/mL respectively, indicating low activity when compared to the positive control. The ethanolic extract of corn silk showed antibacterial activity, mainly on Gram-positive bacteria. The in vitro sun protection factor (SPF) of the ethanolic extract of corn silk incorporated into sunscreen UVA-UVB 5% gel with Pemulen TR-1® was evaluated for the first time. A relative increase in SPF efficacy was observed for the extract when the sunscreen was incorporated. The ethanolic extract of corn silk and the gel were considered non-cytotoxic. The corn silk ethanolic extract was characterized using ultra performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS). Eleven compounds including flavonoids, coumaric acid derivatives, terpenoid, hydroxycinnamic acid derivative and quinic acid derivative were suggested based on their accurate mass and MS/MS spectra.
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    In vitro and in silico investigation of the photoprotective and antioxidant potential of Protium spruceanum leaves and its main flavonoids.
    (2022) Amparo, Tatiane Roquete; Silva, Anne Cherem Peixoto da; Seibert, Janaína Brandão; Silva, Débora dos Santos da; Santos, Viviane Martins Rebello dos; Vieira, Paula Melo de Abreu; Brandão, Geraldo Célio; Souza, Gustavo Henrique Bianco de; Santos, Bianca Aloise Maneira Corrêa
    For the first time, the photoprotective potential (UV absorption and antioxidant properties) of Protium spru- ceanum leaves and its main flavonoids was investigated combining an in vitro and in silico approach. The extract was standardized in rutin content (0.8% w/w), quantified by a validated HPLC-DAD method. The extract pro- tected fibroblasts and keratinocytes against cell death in the stress oxidative H2O2-induced test, with greater effect than rutin standard. This cytoprotection is correlated to the free radical scavenging action, determined by DPPH method. The theoretical investigation on the UV absorption showed an excellent correlation of the results (R2 = 0.911) allowing valuable information about the photoprotective properties of rutin, afzelin and quercitrin, as the importance of the A, B and C rings in the UV absorption property and the hydroxyls in B ring (catechol group) leading to a better antioxidant action. Therefore, the extract from P. spruceanum leaves is a promising candidate for sunscreen development.
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    Photoprotective activity and increase of SPF in sunscreen formulation using lyophilized red propolis extracts from Alagoas.
    (2019) Almeida, Wanessa Aparecida da Silveira; Antunes, Amanda dos Santos; Penido, Ricardo Gonçalves; Correa, Helen Silva da Gloria; Nascimento, Andréa Mendes do; Andrade, Ângela Leão; Santos, Vagner Rodrigues; Cazati, Thiago; Amparo, Tatiane Roquete; Souza, Gustavo Henrique Bianco de; Freitas, Katia Michelle; Santos, Orlando David Henrique dos; Sousa, Lucas Resende Dutra; Santos, Viviane Martins Rebello dos
    The excessive sun exposure, coupled with lack of sun protection represents one of the biggest risks to the occurrence of skin cancer and photoaging. Recent strategies for photoprotection have included the incorporation of natural antioxidant and anti-inflammatory compounds, into sunscreens, and the oral administration of natural antioxidant extracts. In this work, we use Brazilian red propolis extract because its antioxidant and anti-inflammatory activities. The aim of this work was to evaluate the sun protection factor and antioxidant activity of different hydroalcoholic extracts of red propolis (70% and 75%) prepared from lyophilized red propolis at room and high temperatures. The sun protection factor in vitro was determined by a spectrophotometric method developed by Mansur. The hydroalcoholic extracts of red propolis incorporated with Filter UVA-UVB 5% Gel Permulem TR-1 presented absorption in the UVB region. Also, the in vitro capacity of the hydroalcoholic extracts of red propolis to increase photoprotective activity of Filter UVA-UVB 5% Gel was evaluated. The hydroalcoholic extracts of red propolis incorporated presented higher values of sun protection factor and showed synergism in the photoprotective activity of Filter UVA-UVB 5% Gel Permulem TR-1. The antioxidant activity and sun protection factor are correlated with total phenolics content of the extracts and the hydroalcoholic extract of red propolis 75% at room temperature was choosen. The formulation developed with Filter UVA-UVB 5% Gel Permulem TR-1 with this hydroalcoholic extract of red propolis showed safe to be applied on the skin according HET-CAM test. Suggests indication of hydroalcoholic extract of red propolis (75% – room temperature) associated to photoprotective formulations for use in photoprotective products.
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    Seasonality study of essential oil from leaves of Cymbopogon densiflorus and nanoemulsion development with antioxidant activity.
    (2019) Seibert, Janaína Brandão; Rodrigues, Ivanildes Vasconcelos; Carneiro, Simone Pinto; Amparo, Tatiane Roquete; Lanza, Juliane Sousa; Frezard, Frederic Jean Georges; Souza, Gustavo Henrique Bianco de; Santos, Orlando David Henrique dos
    The development of formulations that maintain the biological and physical chemistry properties of essential oils is an important choice when they are used as an active ingredient. This study aimed to characterize the essential oil from leaves of Cymbopogon densiflorus and evaluate the antioxidant activity of the oil, and to produce a nanoemulsion formulation containing it. The essential oil was obtained by hydrodistillation, and seasonality was analysed every 2 months by gas chromatography–mass spectrometry, showing that more than 90% of the composition was maintained for the whole period and that the major compounds were trans‐p‐menta‐2,8‐dien‐1‐ol, cis‐p‐menta‐2,8‐dien‐1‐ol, trans‐p‐menta‐1(7),8‐dien‐2‐ol, cis‐piperitol, and cis‐p‐menta‐1(7),8‐dien‐2‐ol. Stable nanoemulsions were prepared by phase inversion method encapsulating the essential oil. The antioxidant activity was evaluated by 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) free radical scavenging and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulphonic acid (ABTS) methods. In the first test, free and nanoemulsified essential oil showed half‐maximal inhibitory concentration (IC50) values equivalent to 14.689 and 3.692 mg mL−1, respectively. In the second test, these values were 0.567 and 0.43 mg mL−1. The development of nanoemulsion‐based essential oil from leaves of C. densiflorus was viable, and the formulated oil was able to reproduce the antioxidant activity at a concentration four times lower than that of the pure essential oil.
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    Development of propolis nanoemulsion with antioxidant and antimicrobial activity for use as a potential natural preservative.
    (2019) Seibert, Janaína Brandão; Silva, Jessica Paola Bautista; Amparo, Tatiane Roquete; Petit, Alicia; Pervier, Pauline; Almeida, Juliana Cristina dos Santos; Azevedo, Mariana Costa; Silveira, Benila Maria; Brandão, Geraldo Célio; Souza, Gustavo Henrique Bianco de; Teixeira, Luiz Fernando de Medeiros; Santos, Orlando David Henrique dos
    Propolis has demonstrated potential use as food preservative but it presents strong and unpleasant flavor that alters the sensory characteristics foods. A nanoemulsion was proposed to carry the Brazilian propolis extracts for use as natural food preservative. Antimicrobial and antioxidant activities and chemical constituents of the extracts were investigated. The latter were made by sequential extraction using different solvents (hexane, ethyl acetate and ethanol). Antimicrobial activity was evaluated by agar diffusion and microdilution methods and antioxidant activity by DPPH and ABTS assays. Extracts showed antibacterial and antioxidant activity, highlighting the ethanolic which contained artepillin-C, kaempferide, drupanin and p-coumaric acid as main compounds by LC-MS analysis. The nanoemulsion developed by phase inversion method was characterized and stable under thermal-stress and centrifugation conditions. Biological properties evaluated were effectively maintained by the formulation. It was concluded that the nanoemulsion can be used as a food preservative, preventing degradation and masking the propolis off-flavor.
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    Antibacterial activity of extract and fractions from branches of Protium spruceanum and cytotoxicity on fibroblasts.
    (2017) Amparo, Tatiane Roquete; Rodrigues, Ivanildes Vasconcelos; Seibert, Janaína Brandão; Souza, Rafaella Hilda Zaniti; Oliveira, Amanda Ribeiro de; Cabral, Vivette Appolinário Rodrigues; Vieira, Paula Melo de Abreu; Brandão, Geraldo Célio; Okuma, Adriana Akemi; Vieira Filho, Sidney Augusto; Teixeira, Luiz Fernando de Medeiros; Souza, Gustavo Henrique Bianco de
    The crude ethanol extract (CEE) and fractions from branches of Protium spruceanum were subjected to antibacterial and cytotoxicity assays. Compounds of the most active fraction were identified by GC-MS and LC-MS. CEE was active against 19 bacteria and the ethyl acetate fraction (EAF) showed the lowest minimum bactericidal concentration (MBC 0.3–80.0 mg/mL). Through time-kill assay was observed that EAF induced rapid bactericidal effect against Staphylococcus saprophyticus. The cytotoxicity tests against L929 fibroblasts showed great potential of EAF on the treatment of infections caused by five bacteria (MBC < IC50). The results provide in vitro scientific support to the possible application of branches of P. spruceanum as antimicrobial agent that may contribute for treatment of infections.
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    Pseudomonas aeruginosa em hospital da microrregião de Ouro Preto, Minas Gerais, Brasil.
    (2016) Souza, Gustavo Henrique Bianco de; Miranda, Renata Rezende; Inácio, Luciana de Jesus; Amparo, Tatiane Roquete; Vaz, Luana Beatriz Araújo; Fernandes, Maísa Angélica Silva; Mapa, Bruna de Carvalho; Teixeira, Luiz Fernando de Medeiros; Assenço, Regina Aparecida Gomes; Lanna, Maria Célia da Silva; Silva, Alessandra Júlia Santos; Campos, Marcela Maria de Castro; Rios, Elisa Maria Pedrosa da Fonseca
    A água representa o principal meio de transmissão de micro-organismos em centros de saúde, os quais podem causar infecções que são um grave problema de saúde pública. O objetivo desse estudo foi avaliar a qualidade microbiológica da água utilizada em um hospital da microrregião de Ouro Preto, Minas Gerais, Brasil. Foram coletadas doze amostras de água em pontos distintos para testes de detecção de coliformes totais e termotolerantes, assim como de outros microrganismos; além disso, testes de sensibilidade a antimicrobianos também foram realizados. A espécie bacteriana Pseudomonas aeruginosa foi detectada na amostra correspondente à água de enxágue de roupas limpas coletada na área limpa da lavanderia e foi identificada por coloração de Gram e análise automatizada utilizando o equipamento VITEK®. O isolado de P. aeruginosa mostrou-se sensível aos antibióticos usualmente utilizados no tratamento de infecções induzidas por esta bactéria, tais como cefalosporinas, fluorquinolonas, polimixinas e carbapenêmicos. Contudo, os resultados indicam a necessidade de maior atenção ao processo de higienização de roupas hospitalares, fato ainda negligenciado no Brasil.
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    Bioprospecção das atividades antioxidante e antimicrobiana de espécies vegetais medicinais coletadas em Ouro Preto-MG.
    (2013) Souza, Juliana Neves de Paula; Candotti, Julia Gesualdi; Amparo, Tatiane Roquete; Coelho, Fabiana Fioravante; Rodrigues, Ivanildes Vasconcelos; Santos, Orlando David Henrique dos; Medeiros, Luiz Fernando Teixeira de; Furtado, Niege Araçari Jacometti Cardoso; Sousa, Hildeberto Caldas de; Souza, Gustavo Henrique Bianco de
    O presente estudo foi realizado com os extratos etanólicos brutos de seis espécies vegetais (Bathysaaustralis, Piper corcovadensis, Siparuna brasiliensis, Picramnia sp., Piper richardiifolium, Eugenia cf. cerasiflora) de uso medicinal, coletadas na região de Ouro Preto-MG. Os extratos etanólicos foram avaliados frente à atividade antioxidante (método do DPPH) e antimicrobiana (Concentração Inibitória Mínima, CIM), bem como submetidos à dosagem de compostos fenólicos (reagente Folin-Ciocalteau) e flavonóides totais (Cloreto de Alumínio). Os resultados evidenciaram que o extrato de Siparuma brasiliensis apresentou maior atividade antioxidante (CE50 17,712 μg/mL) e o de Piper richardiifolium a melhor atividade antimicrobiana para Staphylococcus aureus (300 μg/mL) e Staphylococcus saprophyticus (200 μg/mL). Estes resultados sugerem que as atividades avaliadas estão diretamente relacionadas com a presença de compostos fenólicos, porém não necessariamente à presença de flavonoides.