Navegando por Autor "Tavares, Juliana Carvalho"

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Inhalation of dimethyl fumarate-encapsulated solid lipid nanoparticles attenuate clinical signs of experimental autoimmune encephalomyelitis and pulmonary inflammatory dysfunction in mice.
(2022) Pinto, Bárbara Fernandes; Ribeiro, Lorena Natasha Brito; Silva, Gisela Bevilacqua Rolfsen Ferreira da; Freitas, Camila Simões de; Rocha, Lucas Kraemer; Oliveira, Fabrício Marcus Silva; Clímaco, Marianna Carvalho; Mourão, Flavio Afonso Gonçalves; Santos, Gabryella Soares Pinheiro dos; Béla, Samantha Ribeiro; Gurgel, Isabella Luísa da Silva; Leite, Fábio de Lima; Oliveira, Anselmo Gomes de; Vilela, Maura Regina Silva da Páscoa; Lima, Onésia Cristina Oliveira; Soriani, Frederico Marianetti; Fujiwara, Ricardo Toshio; Birbrair, Alexander; Russo, Remo de Castro; Tavares, Juliana Carvalho
Rationale: The FDA-approved Dimethyl Fumarate (DMF) as an oral drug for Multiple Sclerosis (MS) treatment based on its immunomodulatory activities. However, it also caused severe adverse effects mainly related to the gastrointestinal system. Objective: Investigated the potential effects of solid lipid nanoparticles (SLNs) containing DMF, administered by inhalation on the clinical signs, central nervous system (CNS) inflammatory response, and lung function changes in mice with experimental autoimmune encephalomyelitis (EAE). Materials and methods: EAE was induced using MOG35–55 peptide in female C57BL/6J mice and the mice were treated via inhalation with DMF-encapsulated SLN (CTRL/SLN/DMF and EAE/SLN/DMF), empty SLN (CTRL/SLN and EAE/SLN), or saline solution (CTRL/saline and EAE/saline), every 72 h during 21 days. Results: After 21 days post-induction, EAE mice treated with DMF-loaded SLN, when compared with EAE/saline and EAE/SLN, showed decreased clinical score and weight loss, reduction in brain and spinal cord injury and inflammation, also related to the increased influx of Foxp3+ cells into the spinal cord and lung tissues. Moreover, our data revealed that EAE mice showed signs of respiratory disease, marked by increased vascular permeability, leukocyte influx, production of TNF-α and IL-17, perivascular and peribronchial inflammation, with pulmonary mechanical dysfunction associated with loss of respiratory volumes and elasticity, which DMF-encapsulated reverted in SLN nebulization. Conclusion: Our study suggests that inhalation of DMF-encapsulated SLN is an effective therapeutic protocol that reduces not only the CNS inflammatory process and disability progression, characteristic of EAE disease, but also protects mice from lung inflammation and pulmonary dysfunction.
A pandemia da COVID-19 como uma questão sociotécnica para a educação científica.
(2021) Viana, Bárbara Mariane Martinez; Silva, Sarah Eliane de Matos; Praça, Patrícia Viotti Leite; Tavares, Juliana Carvalho; Silva, Fábio Augusto Rodrigues e; Coutinho, Francisco Ângelo
O presente artigo tem como interesse analisar aspectos sociotécnicos da pandemia da COVID-19 por meio da descrição de associações entre ciência, tecnologia, sociedade e ambiente. Considerando o cenário pandêmico causado pelo vírus SARS-CoV-2, este estudo se apoia no método de análise da teoria ator-rede, com interesse em questões relacionadas à educação sociocientífica. Para a construção desse estudo, foram elencadas, cronologicamente, as principais ações de combate à COVID-19 no Brasil, no período de janeiro a abril de 2020 e, a partir da análise sobre humanos e não humanos, foi traçada uma rede que evidencia as ações e relações do Ministério da Saúde. Como resultado, vimos as mobilizações que o vírus SARS-CoV-2 provocou em vários campos, como o político, o social, o científico e o tecnológico. A análise de todo esse contexto evidenciou como as conexões de entidades humanas e não humanas vêm gerando novas realidades e diversas incertezas para o futuro. Por meio desta análise pretende-se incentivar a produção de novas intervenções e práticas no ensino de ciências, direcionadas para as necessidades e interesses da sociedade diante do contexto de crises provocado pela pandemia da COVID-19.
Plasmodium berghei NK65 induces cerebral leukocyte recruitment in vivo : an intravital microscopic study.
(2011) Queiroz, Norinne Lacerda; Lima, Onésia Cristina Oliveira; Carneiro, Cláudia Martins; Vilela, Márcia de Carvalho; Teixeira, Antônio Lúcio; Carvalho, Andréa Teixeira de; Araújo, Márcio Sobreira Silva; Martins Filho, Olindo Assis; Braga, Érika Martins; Tavares, Juliana Carvalho
Malaria is second only to tuberculosis as the leading cause of morbidity and mortality as a consequence of a single infectious agent. Much of the pathology of malaria arises from the inappropriate or excessive immune response mounted by the host in an attempt to eliminate the parasite. We here report the inflammatory changes observed in the cerebral microvasculature of C57BL/6 and BALB/c mice that had been inoculated with Plasmodium berghei NK65, a lethal strain of rodent malaria. Although no neurological signs were observed in experimentally infected mice, inflammation of the cerebral microvasculature was clearly evident. Histopathological analysis demonstrated that alterations in cerebral tissue were more intense in infected C57Bl/6 mice than in infected BALB/c animals. Intravital microscopic examination of the cerebral microvasculature revealed increased leukocyte rolling and adhesion in pial venules of infected mice compared with non-infected animals. The extravasation of Evans blue dye into the cerebral parenchyma was also elevated in infected mice in comparison with their non-infected counterparts. Additionally, protein levels of TNF-_, MIG/CXCL9, MCP-1/CCL2, MIP-1_/CCL3 and RANTES/CCL5 were up-regulated in brain samples derived from infected C57Bl/6 mice. Taken together, the data reported here illustrate the complex strain-dependent relationships between leukocyte recruitment, blood brain barrier permeability and chemokine production.