Navegando por Autor "Tagliati, Carlos Alberto"

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Alteration in cellular viability, pro-inflammatory cytokines and nitric oxide production in nephrotoxicity generation by Amphotericin B : involvement of PKA pathway signaling.
(2013) França, Flávia Dayrell; Ferreira, Andrea da Fonseca; Lara, R. C.; Rossoni Júnior, Joamyr Victor; Costa, Daniela Caldeira; Moraes, Karen Cristiane Martinez de; Tagliati, Carlos Alberto; Chaves, Míriam Martins
Amphotericin B is one of the most effective antifungal agents; however, its use is often limited owing to adverse effects, especially nephrotoxicity. The purpose of this study was to evaluate the effect of inhibiting the PKA signaling pathway in nephrotoxicity using Amphotericin B from the assessment of cell viability, pro-inflammatory cytokines and nitric oxide (NO) production in LLC-PK1 and MDCK cell lines. Amphotericin B proved to be cytotoxic for both cell lines, as assessed by the mitochondrial enzyme activity (MTT) assay; caused DNA fragmentation, determined by flow cytometry using the propidium iodide (PI) dye; and activated the PKA pathway (western blot assay). In MDCK cells, the inhibition of the PKA signaling pathway (using the H89 inhibitor) caused a significant reduction in DNA fragmentation. In both cells lines the production of interleukin-6 (IL)-6 proved to be a dependent PKA pathway, whereas tumor necrosis factor-alpha (TNF-α) was not influenced by the inhibition of the PKA pathway. The NO production was increased when cells were pre-incubated with H89 followed by Amphotericin B, and this production produced a dependent PKA pathway in LLC-PK1 and MDCK cells lines. Therefore, considering the present study’s results as a whole, it can be concluded that the inhibition of the PKA signaling pathway can aid in reducing the degree of nephrotoxicity caused by Amphotericin B.
Avaliação da atividade antiulcerogênica da Maytenus truncata Reiss (Celastraceae).
(2005) Silva, R. L; Silva, Renata Pamplona; Martinez, Jorge Rodrigo; Silva, Grácia Divina de Fátima; Vieira Filho, Sidney Augusto; Fonseca, Ana Paula Nascentes de Deus; Tagliati, Carlos Alberto
Maytenus truncata Reiss (Celastraceae) e uma planta nativa da Bahia (Brasil), sendo conhecida como “espinheira-santa”. E usada popularmente na forma de decoto das folhas (chas) como antiulcerogênico, similarmente a Maytenus ilicifolia, a verdadeira “espinheira-santa”. O objetivo do presente estudo foi avaliar a atividade antiulcera e cicatrizante, assim como o perfil fotoquímico dos extratos brutos em acetato de etila e metanol da Maytenus truncata. A administração per os desses extratos nas doses de 120 mg/kg e 240 mg/kg reduziu a severidade da lesão gástrica induzida pelo estresse ao frio (-18 °C por 45 minutos) em ratos, com resultados mais significativos para o extrato bruto obtido em metanol. A administração dos extratos provocou o aumento do pH. Os resultados obtidos na administração do extrato bruto em metanol não contrariam seu uso popular, não somente pela atividade observada, mas também por se tratar de um extrato de alta polaridade cujos princípios podem ser obtidos a partir de uma infusão, embora estudos clínicos devam ser realizados para confirmação dessa hipótese.
Cyclic adenosine monophosphate protects renal cell lines against amphotericin B toxicity in a PKA-independent manner.
(2015) Ferreira, Andrea da Fonseca; França, Flávia Dayrell; Rossoni Júnior, Joamyr Victor; Moraes, Karen Cristiane Martinez de; Gomes, Dawidson Assis; Costa, Daniela Caldeira; Tagliati, Carlos Alberto; Chaves, Míriam Martins
Amphotericin B is the ‘‘gold standard’’ agent in the management of serious systemic fungal infections. However, this drug can cause nephrotoxicity, which contributes up to 25% of all acute kidney injuries in critically ill patients. Cyclic adenosine monophosphate can protect kidney cells from death due to injury or drug exposure in some cases. Hence, the objective of this work was to evaluate if cAMP could prevent cell death that occurs in renal cell lines subjected to AmB treatment and, if so, to assess the involvement of PKA in the transduction of this signal. Two different renal cell lines (LLC-PK1 and MDCK) were used in this study. MTT and flow cytometry assays showed increased cell survival when cells were exposed to cAMP in a PKA-independent manner, which was confirmed by western blot. This finding suggests that cAMP (db-cAMP) may prevent cell death caused by exposure to AmB. This is the first time this effect has been identified when renal cells are exposed to AmB’s nephrotoxic potential.
Estudo fitoquímico do decocto das folhas de maytenus truncata reissek e avaliação das atividades antinociceptiva, antiedematogênica e antiulcerogênica de extratos do decocto.
(2007) Fonseca, Ana Paula Nascentes de Deus; Silva, Grácia Divina de Fátima; Carvalho, Juliana de Jesus; Meléndez Salazar, Gloria Del Carmen; Duarte, Lucienir Pains; Silva, Renata Pamplona; Jorge, Rodrigo Martinez; Tagliati, Carlos Alberto; Zani, Carlos Leomar; Alves, Tânia Maria de Almeida; Peres, Valdir; Vieira Filho, Sidney Augusto
The present paper describes the phytochemical investigation and biological activities of the chloroform, ethyl acetate and methanol extracts of leaf decocts of M. truncata Reiss (Celastraceae). Our studies afforded two flavonoid glycosides, quercetin-3-Orhamnopyranosyl- O-glucopyranosyl-O-rhamnopyranosyl-O-galactopyranoside (1) and kampferol-3-O-rhamnopyranosyl-Oglucopyranosyl- O-rhamnopyranosyl-O-galactopyranoside (2) from the methanolic extract and dulcitol (3) from the ethyl acetate extract. Ethyl acetate and methanol extracts exhibited considerable antiulcerogenic and analgesic activities. The results of the phytochemical studies suggest that the healing activity of methanol extracts can be related to the presence of glycosyl flavonoids.
Role of protein kinase A signaling pathway in cyclosporine nephrotoxicity.
(2014) França, Flávia Dayrell; Ferreira, Andrea da Fonseca; Lara, R. C.; Rossoni Júnior, Joamyr Victor; Costa, Daniela Caldeira; Moraes, Karen Cristiane Martinez de; Gomes, Dawidson Assis; Tagliati, Carlos Alberto; Schultz, Míriam Chaves
Cyclosporine is an important immunosuppressive agent; however, nephrotoxicity is one of the main adverse effects. The purpose of this study was to evaluate the effect of inhibiting the protein kinase A (PKA) signaling pathway in nephrotoxicity caused by cyclosporine from the assessment of cell viability, pro-inflammatory cytokines, and nitric oxide (NO) production in LLC-PK1 and MDCK cell lines. Cyclosporine proved to be cytotoxic for both cell lines, as assessed by the mitochondrial enzyme activity assay (MTT), caused DNA fragmentation, determined by flow cytometry using the propidium iodide dye, and activated the PKA pathway (western blot assay). In MDCK cells, the inhibition of the PKA signaling pathway (H89 inhibitor) caused a significant reduction in DNA fragmentation. In both cell lines, the production of IL-6 proved to be a dependent PKA pathway, while TNF-a was not influenced by the inhibition of the PKA pathway. The NO production was increased when cells were pre-incubated with H89 followed by cyclosporine, and this production was dependent on the PKA pathway in LLC-PK1 and MDCK cells lines. Therefore, considering the present study’s results, it can be concluded that the inhibition of PKA signaling pathway can aid in reducing the degree of nephrotoxicity caused by cyclosporine.