Navegando por Autor "Sousa, Jordano Augusto Carvalho"
Agora exibindo 1 - 4 de 4
Resultados por página
Opções de Ordenação
Item Anti–zika virus activity and isolation of flavonoids from ethanol extracts of Curatella americana L. leaves.(2023) Lima, Lienne D’Auria; Reis, Adriana Cotta Cardoso; Sousa, Jordano Augusto Carvalho; Valente, Gabriel Mendonça; Silva, Breno de Mello; Magalhães, Cíntia Lopes de Brito; Kohlhoff, Markus; Teixeira, Luiz Fernando de Medeiros; Brandão, Geraldo CélioThe ethnomedicinal plant Curatella americana L. (Dilleniaceae) is a common shrub in the Brazilian Cerrado, whose ethanolic extract showed significant in vitro anti–Zika virus activity by the MTT colorimetric method. Currently, there is no drug in clinical use specifically for the treatment of this virus; therefore, in this work, the antiviral and cytotoxic properties of the ethanolic extract, fractions, and compounds were evaluated. The ethanolic extract of the leaves showed no cytotoxicity for the human MRC-5 cell and was moderately cytotoxic for the Vero cell (CC50 161.5 ± 2.01 μg/mL). This extract inhibited the Zika virus multiplication cycle with an EC50 of 85.2 ± 1.65 μg/mL. This extract was fractionated using the liquid–liquid partition technique, and the ethyl acetate fraction showed significant activity against the Zika virus with an EC50 of 40.7 ± 2.33 μg/mL. From the ethyl acetate fraction, the flavonoids quercetin-3-O-hexosylgallate (1), quercetin-3-O-glucoside (2), and quercetin (5) were isolated, and in addition to these compounds, a mixture of quercetin-3- O-rhamnoside (3) and quercetin-3-O-arabinoside (4) was also obtained. The isolated compounds quercetin and quercetin-3-O-hexosylgallate inhibited the viral cytopathic effect at an EC50 of 18.6 ± 2.8 and 152.8 ± 2.0, respectively. Additionally, analyses by liquid chromatography coupled to a mass spectrometer allowed the identification of another 24 minor phenolic constituents present in the ethanolic extract and in the ethyl acetate fraction of this species.Item Anti–Zika virus activity and isolation of flavonoids from ethanol extracts of Curatella americana L. Leaves.(2023) Lima, Lienne D’Auria; Reis, Adriana Cotta Cardoso; Sousa, Jordano Augusto Carvalho; Valente, Gabriel Mendonça; Silva, Breno de Mello; Magalhães, Cíntia Lopes de Brito; Kohlhoff, Markus; Teixeira, Luiz Fernando de Medeiros; Brandão, Geraldo CélioThe ethnomedicinal plant Curatella americana L. (Dilleniaceae) is a common shrub in the Brazilian Cerrado, whose ethanolic extract showed significant in vitro anti–Zika virus activity by the MTT colorimetric method. Currently, there is no drug in clinical use specifically for the treatment of this virus; therefore, in this work, the antiviral and cytotoxic properties of the ethanolic extract, fractions, and compounds were evaluated. The ethanolic extract of the leaves showed no cytotoxicity for the human MRC-5 cell and was moderately cytotoxic for the Vero cell (CC50 161.5 ± 2.01 μg/mL). This extract inhibited the Zika virus multiplication cycle with an EC50 of 85.2 ± 1.65 μg/mL. This extract was fractionated using the liquid–liquid partition technique, and the ethyl acetate fraction showed significant activity against the Zika virus with an EC50 of 40.7 ± 2.33 μg/mL. From the ethyl acetate fraction, the flavonoids quercetin-3-O-hexosylgallate (1), quercetin-3-O-glucoside (2), and quercetin (5) were isolated, and in addition to these compounds, a mixture of quercetin-3- O-rhamnoside (3) and quercetin-3-O-arabinoside (4) was also obtained. The isolated compounds quercetin and quercetin-3-O-hexosylgallate inhibited the viral cytopathic effect at an EC50 of 18.6 ± 2.8 and 152.8 ± 2.0, respectively. Additionally, analyses by liquid chromatography coupled to a mass spectrometer allowed the identification of another 24 minor phenolic constituents present in the ethanolic extract and in the ethyl acetate fraction of this species.Item Fitoquímica, citotoxicidade e atividade anti-Zika vírus in vitro de extratos etanólicos de espécies da família Rutaceae.(2020) Sousa, Jordano Augusto Carvalho; Brandão, Geraldo Célio; Brandão, Geraldo Célio; Souza, Gustavo Henrique Bianco de; Magalhães, José Carlos deExtratos etanólicos de folhas e caules das espécies Rauia resinosa, Galipea jasminiflora, Dictyoloma vandellianum e Zanthoxylum riedelianum foram submetidos à uma triagem fitoquímica preliminar. Os extratos foram obtidos por percolação exaustiva com etanol (92,8° GL) e analisados por CCD para identificação de metabólitos secundários presentes. Foi empregada cromatografia líquida de ultra eficiência em fase reversa, acoplada com detector de arranjo de diodos e espectrometria de massas para identificação de compostos tais como flavonas polimetoxiladas e alcaloides furoquinolínicos. Do extrato etanólico das folhas de Rauia resinosa foram isoladas cinco substâncias: β-sitosterol, 5-hidróxi-3´,4´- metilenodioxi,5´,6,7-trimetoxiflavona inédita na literatura, 5,7,3´,4´,5´-pentametoxiflavona, 5,7,8-trimetoxi-3´,4´-metilenodioxiflavona e 5,7,8,3´,4´-pentametoxiflavona. As substâncias tiveram suas estruturas elucidadas por RMN 13C, 1H, DEPT 135, HSQC, HMBC e espectrometria de massas. A atividade citotóxica in vitro de 14 amostras (extratos etanólicos de folhas e caules das espécies, frações e substâncias isoladas) foi determinada para células: MRC5 (fibroblasto pulmonar), T24 (carcinoma de bexiga) TOV-21-G (adenocarcinoma de ovário) e HepG2 (carcinoma hepático), utilizando o método do MTT para determinação da CC50. Os ensaios de atividade antiviral foram realizados para os extratos etanólicos de folhas e caules da espécie Rauia resinosa, frações e substâncias isoladas, totalizando 10 amostras testadas em células Vero infectadas pelo ZIKV e avaliadas pelo método do MTT para a determinação da CE50. Das 14 amostras avaliadas no ensaio de citotoxicidade, nove apresentaram atividade antiproliferativa contra as linhagens celulares com índices de seletividade superiores a 2,0. Destas, destacam-se a fração diclorometânica dos caules de Rauia resinosa com CC50 = 21,20 µg/mL (IS =5,01) para a linhagem HepG2 e o extrato etanólico das folhas de Galipea jasminiflora com CC50 = 31,98 µg/mL (IS =4,84) também para a linhagem HepG2. 5,7,8,3´,4´- pentametoxiflavona apresentou atividade citotóxica para as linhagens celulares T24 (CC50 = 30,22 µg/mL), (IS =2,42) e TOV-21-G (CC50 = 29,16 µg/mL), (IS =2,52). Das 10 amostras avaliadas, duas apresentaram atividade anti-ZIKV com índices de seletividade inferiores a 2,0, o extrato etanólico dos caules de Rauia resinosa (CE50 = 184,50 µg/mL), (IS =1,15) e a fração diclorometânica das folhas de Rauia resinosa (CE50 = 51,13 µg/mL), (IS =1,29).Item High-resolution mass spectrometry identification and characterization of flavonoids from Fridericia chica leaves extract with anti-arbovirus activity.(2022) Cruz, Ana Flávia Gomes da; Reis, Adriana Cotta Cardoso; Sousa, Jordano Augusto Carvalho; Vaz, Luana Beatriz Araújo; Silva, Breno de Mello; Magalhães, Cíntia Lopes de Brito; Kohlhoff, Markus; Oliveira, Alaíde Braga de; Brandão, Geraldo CélioPlant extracts are complex mixtures that are difficult to characterize, and mass spectrometry is one of the main techniques currently used in dereplication processes. Fridericia chica is a species with medicinal uses in Latin American countries, used in the treatment of inflammatory and infectious diseases. Extracts of this plant species are characterized by the presence of anthocyanidins. In this study, using high-resolution mass spectrometry coupled with liquid chromatography, it was possible to determine the molecular formula of thirty-nine flavonoids. Fragmentation analysis, ultraviolet spectrum and nuclear magnetic resonance data allowed the partial characterization of the structures of these compounds. The spectral dataset allowed the identification of a series of flavones in addition to the desoxyanthocyanidins common in extracts of the species. The occurrence of some of the proposed structures is uncommon in extracts of species of the Bignoniaceae family, and they are reported for the first time in the extract of this species. Quantitative analyses of total flavonoids confirmed the high content of these constituents in the species, with 4.09 ± 0.34 mg/g of dry plant material. The extract under study showed low in vitro cytotoxicity with CC50 ≥ 296.7 ± 1.4 μg/mL for Vero, LLC-MK2 and MRC-5 cell lines. In antiviral activity assays, inhibition of the cytopathic effects of Dengue, Zika and Mayaro viruses was observed, with EC50 values ranging between 30.1 and 40.9 μg/mL. The best result was observed against the Mayaro virus, with an EC50 of 30.1 μg/mL.