Navegando por Autor "Silva, Messias Borges"

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Adjustment of the operational parameters of an unconventional integrated and illuminated internally photobioreactor (ILI-PBR) for the batch autotrophic cultivation of the Chlorella minutissima, using the taguchi method.
(2020) Amaral, Mateus de Souza; Loures, Carla Cristina Almeida; Silva, Messias Borges; Prata, Arnaldo Márcio Ramalho
Microalgae crops targeting biotechnological applications are conducted in photobioreactors, which allow the adjustment and control of parameters of luminosity, agitation and mixing in the cultivation medium to promote better cell growth and accumulation of metabolites. In this sense, the present work used the Taguchi method to find the best adjustment of the operational parameters of an unconventional photobioreactor denominated internally illuminated integrated photobioreactor (ILI- PBR) with LED light aiming at elevating the biomass concentration, volumetric biomass productivity, and volumetric lipid productivity of Chlorella minutissima microalgae cultivated under autotrophic regime. The effects of the factors were evaluated: illumina- tion (blue, white and red); photoperiod (12 h light:12 h dark, 1 h light:1 h dark, and 24 h clear:0 h dark); aeration (0, 3 vvm, 0.4 vvm, and 0.5 vvm); and recirculation flow rate of cultivation medium (5 L min−1, 6.5 L min−1, and 9 L min−1) on the variable responses: biomass concentration, biomass volumetric productivity, lipid content, and volumetric lipid productivity. The use of the Taguchi method allowed the increase of biomass concentration, volumetric biomass productivity, and volumetric lipid productivity in the biomass of Chlorella minutissimain 8.6%, 42%, and 143%, respectively, with the adjust- ment of the operational parameters of the photobioreactor used.
An unconventional two-stage cultivation strategy to increase the lipid content and enhance the fatty acid profile on Chlorella minutissima biomass cultivated in a novel internal light integrated photobioreactor aiming at biodiesel production.
(2020) Amaral, Mateus de Souza; Loures, Carla Cristina Almeida; Pedro, Guilherme Arantes; Reis, Cristiano Eduardo Rodrigues; Castro, Heizir Ferreira de; Naves, Fabiano Luiz; Silva, Messias Borges; Prata, Arnaldo Márcio Ramalho
Biodiesel quality is dependent upon the fatty acids distribution of the raw material used in the trans/ esterification reactions. While microalgae cells are regarded as potential oil suppliers for the biodiesel production chain, a common criticism is related to the low lipid productivities and often to a poor fatty acid distribution. In the present work we propose to submit microalgal cultivation to a second stage with nutritional stress by a simple addition of a 70% nitrate source depleted medium at the beginning of stationary phase, without harvesting cells, saving time and water. Chlorella minutissima was cultivated in a novel unconventional photobioreactor, named Internally Illuminated Integrated Photobioreactor (ILI- PBR), to verify the efficiency of the proposed strategy. Biomass concentration, lipid content and volu- metric lipids productivity increased 24.9%, 120.7% and 55.6%, respectively, from Stage 1 to Stage 2, when ILI-PBR was illuminated by constant blue LED and 0.5 vvm of aeration. The fatty acid profiles, evaluated in terms of LED illumination (white, red and blue), showed a predominance of palmitic acid in all cases (59.49%, 57.28% and 46.51%, respectively), which allowed to enhance some biodiesel properties such as cetane number, cold filter plugging point, density and kinematic viscosity, according to international specifications.
Development of a novel nano-biomaterial for biomedical applications.
(2018) Machado, Vagner de Oliveira; Andrade, Ângela Leão; Simon, Alice; Fernández, Daniel Ernesto Rodríguez; Fabris, José Domingos; Domingues, Rosana Zacarias; Silva, Raphael Ferreira da; Silva, Thamiris Caroline Esteves; Peixoto, Thainá Lopes; Santos, Cláudio Teodoro dos; Motta, Adriana Cristina; Duek, Eliana Aparecida de Rezende; Silva, Messias Borges; Gomes, Alaelson Vieira; Cabral, Lúcio Mendes; Carmo, Flávia Almada do; Elias, Carlos Nelson
The technological development of biodegradable nanoparticles for drug delivery in medical diagnostics and therapies is addressing increasing efforts to make safer, affordable and more bio-efficient polymeric materials. The preparation via the solvent displacement method of a novel polymeric nanomaterial with new spherical and narrow sizes dispersion is described. It was achieved by choosing the best amount of PLDLA-co-TMC, PEO-PPO-PEO and an adequate acetone:methanol volume ratio, using the factorial design 23 and Box-Behnken experiment design. This new material was loaded with finasteride, the chosen drug model, and its delivery dynamic was determined by the HPLC method. Results revealed that an optimal formulation of PLDLA-co-TMC nanoparticles was found to be composed of 20 mg PLDLA-co-TMC; 37.5 mg PEO-PPO-PEO and a volume acetone:methanol ratio 2.2:0.3. The formulation of this novel biomaterial showed a high encapsulation efficiency of finasteride, controlled release profile along 24 h, without burst effect in the first 4 h, nanometric sizes, spherical shapes, close polydispersity, negative surface charge, unimodal particle size distribution and relatively high thermal stability of the polymer matrix PEO-PPO-PEO/PLDLA-co-TMC. These results support the fabrication of technologically advanced nanoparticles based on high molecular weight PLDLA-co-TMC and their use in the nano-controlled drug release in biomedical applications.
Evaluation of cell growth performance of microalgae Chlorella minutissima using an internal light integrated photobioreactor.
(2020) Amaral, Mateus de Souza; Loures, Carla Cristina Almeida; Naves, Fabiano Luiz; Silva, Messias Borges; Prata, Arnaldo Márcio Ramalho
Microalgae have received special attention due to the biotechnological potential of their biomass, which can be used to produce biofuels, food formulations and pharmaceutical products. In order to improve biomass productivity, the present work proposes an internal light integrated photobioreactor (ILI-PBR), which integrates two configurations of photobioreactors (PBRs) already consecrated and widely used (tubular and bubble column) in a single system, combining the benefits of each configuration in a single PBR. To evaluate the applicability of ILI-PBR, a comparative test was conducted with the internal light bubble column photobioreactor (ILBC-PBR); both PBRs were illuminated with blue light-emitting diodes (LED).The PBR’s performance was evaluated in terms of specific growth rate (µmax) and cell productivity (PX) of the microalgae Chlorella minutissima. The µmax and PX in ILI-PBR were at least 42.85 and 58.06 % higher than ILBC-PBR, respectively. ILI-PBR was 36 % more efficient in terms of electricity consumption than ILBC-PBR.
Highly wet Chlorella minutissima biomass for in situ biodiesel production and residual biomass rich in labile carbohydrates.
(2022) Pedro, Guilherme Arantes; Amaral, Mateus de Souza; Pereira, Félix Monteiro; Flumignan, Danilo Luiz; Rós, Patrícia Caroline Molgero da; Reis, Cristiano Eduardo Rodrigues; Silva, Messias Borges
This study evaluated the use of biomass of Chlorella minutissima grown in 40-L photobioreactors using a Guillard f/2 biomass at over 80% moisture content as feedstock to produce ethyl esters. The study design investigated the infuence of acid catalyst type (HCl or H2SO4) and concentration (0.3 or 0.6 mol L−1), co-solvent to ethanol ratio (1:10 or 1:5 v/v), and acyl acceptor to biomass ratio (25 mL g−1 or 50 mL g−1) on ester yield and conversion. An L8 Taguchi design was used to evaluate the infuence of the factors, demonstrating a range of conversion ranging from 37 to 98%. Furthermore, the efects of reaction conditions on the residual biomass were assessed by measuring the concentration of acid-labile carbohydrates, which could be utilized in integrated sugar- and lipid-based algal refneries. It was found that in situ transesterifcation using HCl can provide easily accessible and fermentable carbohydrates at concentrations of about 32 wt% in residual biomass. In this sense, the fndings of this study demonstrate a further step in the integration of microalgae biomass in the development of liquid-fuel biorefneries.
Modeling and optimization of hybrid leachate treatment processes and scale-up of the process : review.
(2021) Vaz, Carlos Alberto dos Santos; Samanamud, Gisella Lamas; Silva, Ricardo Soares da; França, Alexandre Bôscaro; Quintão, Cristiane Medina Finzi; Urzedo, Ana Paula; Silva, Messias Borges; Bosch Neto, Juan Canellas; Amaral, Mateus de Souza; Loures, Carla Cristina Almeida; Naves, Luzia Lima Rezende; Naves, Fabiano Luiz
Optimized models of landfill leachate processes that could lead to the production of fuel oils from the pyrolysis of plastics and cultivation of microalgae, can be high-efficient and sustainable activities. Studies using advanced oxidation processes in combination with biological processes showed to be efficient in treating leachate. The complexity of this type of waste, as well as the continuous modification of its properties due to local seasonality, prevent kinetic and thermodynamic studies to predict the steady state at the final disposal. Environmental management system using Lean Six sigma accompanied with process modeling and optimization tools, whether statistical or computational, generate reliable models. These models can reproduce reductions on contaminants by confirming the initial conditions of each leachate enabling the scale-up of the process. This Review provides data survey, types of treatment and models linked to optimization tools in wastewater decontamination processes from municipal solid waste (MSW). This review includes the synthesis and proposal for treatment and man- agement from of the classification of the garbage until the generation of leachate, aiming to contributing to future researches in the leachate treatment.