Navegando por Autor "Silva, Fábio Henrique Rodrigues da"

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    Miconazole-loaded nanoparticles coated with hyaluronic acid to treat vulvovaginal candidiasis.
    (2023) Teixeira, Aniely dos Reis; Quaresma, Amanda de Vasconcelos; Branquinho, Renata Tupinambá; Santos, Stephanie Lourrani Evangelista Neves; Magalhães, Juliana Teixeira; Silva, Fábio Henrique Rodrigues da; Marques, Maria Betânia de Freitas; Moura, Sandra Aparecida Lima de; Barboza, Ana Paula Moreira; Araújo, Marcelo Gonzaga de Freitas; Silva, Gisele Rodrigues da
    Miconazole-loaded nanoparticles coated with hyaluronic acid (miconazole-loaded nanoparticles/HA) were developed to overcome the limitations of the conventional therapy of the vulvovaginal candidiasis (VVC). They were synthesized by emulsification and solvent evaporation techniques, characterized by diameter, poly- dispersity index, zeta potential, encapsulation efficiency, atomic force microscopy (AFM), evaluated in terms of efficacy against C. albicans in vitro, and tested in a murine VVC model. Nanoparticles showed 211nm of diameter with a 0.32 polydispersity index, -53mV of zeta potential, and 90% miconazole encapsulation efficiency. AFM evidenced nanoparticles with a spherical shape. They inhibited the proliferation of C. albicans in vitro and in vivo after a single administration. Nanoparticles released the miconazole directly in the site of action at low thera- peutic doses, which was enough to eliminate the fungal burden in the murine VVC model. These systems were rationally designed since the existence of the HA induces their adhesion on the vaginal mucus and their inter- nalization via CD44 receptors, inhibiting the C. albicans. Therefore, miconazole-loaded nanoparticles/HA represent an innovative non-conventional pharmaceutical dosage form to treat the VVC and recurrent VVC.
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    Sodium butyrate-loaded nanoparticles coated with chitosan for the treatment of neovascularization in age-related macular degeneration : ocular biocompatibility and antiangiogenic activity.
    (2022) Reis, Júlia Stephania dos; Teixeira, Aniely dos Reis; Quaresma, Amanda de Vasconcelos; Almeida, Tamires Cunha; Arribada, Raquel Gregorio; Neto, Julia Teixeira; Silva, Fábio Henrique Rodrigues da; Cunha Júnior, Armando da Silva; Moura, Sandra Aparecida Lima de; Silva, Glenda Nicioli da; Fialho, Sílvia Ligório; Silva, Gisele Rodrigues da
    Sodium butyrate-loaded nanoparticles coated chitosan (NaBu-loaded nanoparticles/CS) were developed to treat the choroidal neovascularization in wet age-related macular degeneration (AMD). The nanoparticles were produced by double emulsification and solvent evaporation technique, optimized by experimental statistical design, characterized by analytical methods, investigated in terms of in vitro and in vivo ocular biocompatibility, and evaluated as an antiangiogenic system in vivo. The NaBu-loaded nanoparticles/CS were 311.1 ± 3.1 nm in diameter with a 0.208 ± 0.007 polydispersity index; had a +56.3 ± 2.6 mV zeta potential; showed a 92.3 % NaBu encapsulation efficiency; and sustained the drug release over 35 days. The NaBu-loaded nanoparticles/CS showed no toxicity to human retinal pigment epithelium cells (ARPE-19 cells); was not irritant to the chorioallantoic membrane (CAM); did not interfere in the integrity of the retinal layers of rat’s eyes, as detected by the Optical Coherence Tomography and histopathology; and inhibited the angiogenesis in CAM assay. The NaBu-loaded nanoparticles/CS could be a therapeutic alternative to limit the neovascularization in AMD.