Navegando por Autor "Santos, Marcelo Cordeiro dos"

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    Detection of drug resistant mycobacterium tuberculosis strains using Kit SIRE Nitratase® : a multicenter study.
    (2020) Miranda, Silvana Spindola de; Almeida, Isabela Neves de; Mansur, Maria de Fátima Filardi Oliveira; Figueredo, Lida Jouca de Assis; Carvalho, Wânia da Silva; Hadaad, João Paulo Amaral; Diniz, Jaciara de Lourdes do Carmo Guimarães; Groll, Andrea von; Silva, Pedro Almeida da; Lopes, Maria Luiza; Santos, Marcelo Cordeiro dos; Brito, Alexandra; Mello, Fernanda Carvalho de Queiroz; Malaquias, Thiago da Silva Santos; Croda, Julio; Pinhata, Juliana Maira Watanabe; Oliveira, Rosângela Siqueira de; Chimara, Erica; Rossetti, Maria Lúcia; Halon, Maria Laura; Lourenço, Maria Cristina; Medeiros, Reginalda Ferreira de Melo; Montes, Fátima Cristina Onofre Fandinho; Machado, Diana; Viveiros, Miguel; Kritski, Afrânio Lineu
    (1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.
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    Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil.
    (2022) Lima, Ana Carolina de Oliveira de; Schmid, Karen Barros; Melo, Hilda Ferreira de; Athayde, Rafaella Christine; Monte, Rossiclea Lins; Almeida, Isabela Neves de; Miranda, Silvana Spíndola de; Kritski, Afrânio; Rossetti, Maria Lucia Rosa; Santos, Marcelo Cordeiro dos
    Background: In recent years, the prevalence of nontuberculous mycobacterial (NTM) infections has increased in different regions of the world. The American Thoracic Society (ATS) recommends standardized identification criteria, reinforcing the need for faster and less complicated clinical and laboratory techniques. Methods: In this retrospective study, NTM species isolated from pulmonary, extrapulmonary, and disseminated samples from patients treated at a TB/HIV reference unit in the State of Amazonas from 2011 to 2014 were identified through a combination of molecular techniques. Results: To identify the molecular technique, 50 cryopreserved NTM cultures were recovered and subcultivated in culture medium. The potentially pathogenic NTM species identified were M. avium, M. intracellulare, M. kansasii, M. chelonae, M. abscessus, M. fortuitum, and M. peregrinum. Results of GenoType® showed moderate agreement with those of genomic sequencing (kappa = 0.60), whereas the results obtained by the PRA-hsp65 technique disagreed with the results obtained by sequencing (kappa = 0.49). Conclusions: Our findings highlight that GenoType CM is a good method for the identification of NTM, as well as the need for the application of standardized criteria, such as those set forth by the ATS.