Navegando por Autor "Santos, Daisy Motta"

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Angiotensin-(1–7) oral formulation improves physical performance in mountain bike athletes : a double‐blinded crossover study.
(2021) Moura, Samara Silva de; Mendes, Adália Táci Pereira; Martins Junior, Francisco de Assis Dias; Totou, Nádia Lúcia; Coelho, Daniel Barbosa; Oliveira, Emerson Cruz de; Santos, Daisy Motta; Santos, Robson Augusto Souza dos; Oliveira, Lenice Kappes Becker
Background: The ECA2/Ang-(1–7)/Mas axis is shown to be involved in effects mediated by physical exercise, as it can induce the release of nitric oxide (ON) and bradykinin (BK), which are potent vasodilators. The vasodilating action the NO/BK can contribute to increased metabolic efficiency in muscle tissue and central nervous system. The formulation HPβ-CD-Ang-(1–7) through its mechanisms of action can be a promising supplement to aid in the maintenance and improvement of performance and may also favor recovery during competitions. The premise of this study was to investigate the effects of acute oral supplementation HPβ-CD-Ang-(1–7) on the performance of mountain bike (MTB) practitioners. Methods: Fourteen recreational athletes, involved in training programs for at least one year, participated in this crossover design study. Subjects underwent two days of testing with a seven-day interval. HPβ-CD-Ang-(1–7) (1.75 mg) and HPβCD-Placebo were provided in capsules three hours prior to tests. To determine the safety of the HPβ-CD-Ang-(1–7) formulation associated with physical effort, cardiovascular parameters heart rate (HR) and blood pressure (BP) were analyzed. Physical performance was measured using maximal oxygen uptake (VO2), total exercise time (TET), mechanical work (MW), mechanical efficiency (ME), and rating of perceived exertion (RPE). Respiratory exchange coefficient (REC), lactate and non-esterified fatty acids (NEFAs) were measured. Maximal incremental tests were performed on a progressively loaded leg cycle ergometer. Results: There were no significant differences in terms of HR or BP at rest and maximum effort between the HPβ-CD-Ang- (1–7) and placebo groups. The VO2max showed significant differences (p = 0.04). It was higher in the Ang-(1–7)condition (66.15 mlO2.kg− 1 .min− 1 ) compared to the placebo (60.72 mlO2.kg− 1 .min− 1 ). This was also observed for TET (Ang-(1–7) 39.10 min vs. placebo 38.14 min; p = 0.04), MW (Ang-(1–7) 156.7 vs. placebo 148.2; p = 0.04), and at the lowest RPE (Ang-(1–7) vs. placebo; p = 0.009). No significant differences were observed for REC, NEFAs, or Lactate. Conclusions: These results suggest that HPβ-CD-Ang-(1–7) improves the physical performance of MTB recreational athletes and could be a promising supplement. Trial registration: RBR-2 × 56pw8, registered January 15th, 2021. The study was prospectively registered.
Células imunes e marcadores inflamatórios em indivíduos submetidos a uma sessão de treinamento de força.
(2018) Pontes, Washington Martins; Silva, André Talvani Pedrosa da; Pinto, Kelerson Mauro de Castro; Santos, Daisy Motta; Silva, André Talvani Pedrosa da; Costa, Daniela Caldeira; Peixoto, Marco Fabrício Dias; Pinto, Kelerson Mauro de Castro
O exercício físico representa um desafio a homeostase corporal, influenciando inclusive o funcionamento do sistema imunológico. Estudos tem demonstrado relação entre a resposta inflamatória e o processo de remodelamento tecidual, porém mais estudos são necessários para compreendermos seu comportamento e sua atuação neste processo adaptativo após treinamento de força. O objetivo do estudo foi avaliar o perfil das células imunes e da expressão gênica de biomarcadores inflamatórios solúveis nas PBMC após uma sessão de treinamento de força, que objetivou a hipertrofia muscular esquelética, em indivíduos treinados e não treinados. Os protocolos foram constituídos por 3 exercícios, leg press, extensor de joelhos sentado e flexor de joelhos, sendo realizadas 4 séries, de 8-10 repetições, a 65% de 1RM e com uma pausa de 90s entre as séries. A duração da repetição foi de 5 segundos (2 segundos para a ação muscular concêntrica por 3 segundos para a ação muscular excêntrica). Foram realizadas análises no sangue antes, logo após e 2 horas após o término do protocolo de exercício. O nível de lactato sanguíneo aumentou logo após o exercício para ambos os grupos e a atividade da enzima creatina quinase aumentou logo após ao exercício. Observamos um aumento dos linfócitos circulantes imediatamente após o exercício enquanto sua diminuição foi detectada 2 horas após o término do mesmo. Os monócitos apresentam seu pico na circulação logo após o exercício físico enquanto os neutrófilos elevaram-se com o exercício, sendo observada no grupo não treinado, um aumento ainda mais evidente 2 horas após o término do treinamento. Não encontramos diferenças entre os grupos treinado e não treinado quanto à expressão gênica de IL6, IL10, IL12, IFN-y nas PBMCs, assim como não encontramos diferença no decorrer do tempo. Porém, o grupo treinado destacou-se por apresentar menor expressão de TGFβ nestas células. Concluímos que o protocolo de exercício utilizado, seguindo as normativas de carga com objetivo de hipertrofia muscular esquelética, em jovens treinados e não treinados, eleva os níveis de lactato sanguíneo, da atividade da CK, dos leucócitos circulantes e, inversamente no caso dos jovens treinados, reduz a expressão de TGFβ nas PBMC.
Discovery and characterization of Alamandine : a novel component of the Renin-Angiotensin system.
(2013) Lautner, Roberto Queiroga; Villela, Daniel Campos; Silva, Rodrigo Araújo Fraga da; Silva, Neiva Caldeira; Braga, Thiago Verano; Fraga, Fabiana Costa; Jankowski, Joachim; Jankowski, Vera; Sousa, Frederico Barros de; Alzamora, Andréia Carvalho; Soares, Everton Rocha; Barbosa, Claudiane Maria; Kjeldsen, Frank; Oliveira, Aline Cristina; Braga, Janaina Félix; Savergnini, Silvia Silveira Quintão; Etelvino, Gisele Maia; Peluso, Antonio Augusto Bastos; Silva, Danielle Gomes Passos; Ferreira, Anderson José; Alves, Fabiana; Martins, Almir de Sousa; Raizada, Mohan K.; Paula, Renata Dutra de; Santos, Daisy Motta; Klempin, Friederike; Pimenta, Adriano Monteiro de Castro; Alenina, Natalia; Sinisterra Millán, Ruben Dario; Bader, Michael; Santos, Maria José Campagnole dos; Santos, Robson Augusto Souza dos
The renin–angiotensin system (RAS) is a key regulator of the cardiovascular system, electrolyte, and water balance. Here, we report identification and characterization of alamandine, a new heptapeptide generated by catalytic action of angiotensin-converting enzyme-2 angiotensin A or directly from angiotensin-(1–7). To characterize a novel component of the RAS, alamandine. Using mass spectrometry we observed that alamandine circulates in human blood and can be formed from angiotensin-(1–7) in the heart. Alamandine produces several physiological actions that resemble those produced by angiotensin-(1–7), including vasodilation, antifibrosis, antihypertensive, and central effects. Interestingly, our data reveal that its actions are independent of the known vasodilator receptors of the RAS, Mas, and angiotensin II type 2 receptor. Rather, we demonstrate that alamandine acts through the Mas-related G-protein–coupled receptor, member D. Binding of alamandine to Mas-related G-protein–coupled receptor, member D is blocked by D-Pro7-angiotensin-(1–7), the Mas-related G-protein–coupled receptor, member D ligand β-alanine and PD123319, but not by the Mas antagonist A-779. In addition, oral administration of an inclusion compound of alamandine/β-hydroxypropyl cyclodextrin produced a long-term antihypertensive effect in spontaneously hypertensive rats and antifibrotic effects in isoproterenol-treated rats. Alamandine had no noticeable proliferative or antiproliferative effect in human tumoral cell lines. The identification of these 2 novel components of the RAS, alamandine and its receptor, provides new insights for the understanding of the physiological and pathophysiological role of the RAS and may help to develop new therapeutic strategies for treating human cardiovascular diseases and other related disorders.
Eccentric overload muscle damage is attenuated by a novel angiotensin- (1-7) treatment.
(2018) Oliveira, Lenice Kappes Becker; Totou, Nádia Lúcia; Moura, Samara Silva de; Kangussu, Lucas Miranda; Sinisterra Millán, Ruben Dario; Santos, Maria Jose Campagnole dos; Coelho, Daniel Barbosa; Santos, Daisy Motta; Santos, Robson Augusto Souza dos
The development of new strategies to attenuate exercise-induced muscle damage may be helpful for training regimens. The aim of this study was to determine whether a oral formulation of angiotensin Ang-(1-7)[HPβCD/Ang-(1-7)] is effective to reduce pain, and muscle damage markers after eccentric-overload exercise. HPβCD (Placebo) and HPβCD/Ang-(1-7) (Ang-(1-7) group were treated for 7 days (one capsule/day). The pain was measured by visual analogue scale, maximal strength (MS) using force platform. Blood samples were collected for cytokines and creatine kinase (CK) analysis. The Ang-(1-7)-treated group reported less pain immediately (3.46± 0.64 vs. placebo 3.80± 0.77 cm) and 24h after exercise (3.07±0.71 vs. 3.73±0.58cm placebo) and higher MS at 24h (24±12N) and 48h (30±15N) vs. placebo (-8 ± 9 N and -10 ± 9 N). The CK for Ang-(1-7) (0.5 ± 0.1 and 0.9±0.2 U/L) were lower at 48 and 72h vs. placebo (fold changes of 1.7±0.5 and 1.5±0.3 U/L). The TNF-α level was lower in the treated group post-exercise (38 ± 2.5 pg/ml) vs. placebo (45 ± 2.9 pg/ml) but no significant changes were observed for IL-6 and IL-10. Our data indicate that treatment with Ang-(1-7) may attenuate pain, some of the muscle damage markers and improves performance following eccentric exercise.
Efeito da formulação oral HPβCD-Angiotensina-(1-7) na lesão muscular e rendimento físico de atletas de mountain bike : um estudo crossover duplo-cego.
(2020) Moura, Samara Silva de; Oliveira, Lenice Kappes Becker; Santos, Daisy Motta; Oliveira, Lenice Kappes Becker; Santos, Daisy Motta; Gonçalves, Reginal; Silva, Fernanda Guimarães Drummond e
Constantemente, pesquisas buscam associação de novos suplementos no rendimento esportivo. Estudos prévios em animais e humanos sugerem que a formulação oral HPβCD-Angiotensina-(1-7) poderia aumentar o rendimento físico, visto que induz a liberação de óxido nítrico e potencializa a ação da bradicinina. Além disso, a Ang-(1-7) pode estar envolvida na utilização de ácidos graxos não esterificados liberados dos adipócitos, o que possivelmente pode contribuir para maior β-oxidação, poupando a glicose hepática e muscular, contribuindo, dessa forma, para uma menor variação de glicose plasmática, sugerindo uma melhoria global na eficiência metabólica. A premissa deste estudo foi investigar o efeito da suplementação oral HPβCD-Angiotensina-(1-7) no rendimento esportivo de atletas de mountain bike (MTB). Participaram do estudo 10 atletas de MTB da região de Ouro Preto/MG, envolvidos em seus programas de treinamento por pelo menos um ano. Os ensaios experimentais foram delineados de maneira cruzada, dupla-cega, randomizada, separados por 7 dias. A administração oral de HPβCD-Ang-(1-7) (2mg) e HPβCD-Placebo em cápsulas, 3 horas antes dos testes. Para investigar os efeitos da formulação HPβCD-Ang-(1-7) em parâmetros cardiovasculares, foram avaliadas a frequência cardíaca e a pressão arterial em repouso e no pico de esforço máximo. O rendimento físico foi avaliado por meio de um teste de contrarrelógio (TT20km) em um cicloergomêtrico de pernas. Medidas perceptivas foram consideradas: percepção subjetiva de esforço (PSE) foi registrada a cada 2 km, durante os 20km e, percepção subjetiva de esforço da sessão de teste (sPSE). Amostras de sangue foram colhidas pré, pós, 24 e 48h após os testes físicos, para avaliar os parâmetros bioquímicos: lactato, ácidos graxos não esterificados (AGNEs), glicemia e creatina quinase (CK). Os dados foram expressos em média ± desvio padrão da média e o nível de significância adotado foi de p < 0,05. Não foram observadas diferenças significativas para as variáveis de rendimento físico, medidas de percepção do esforço físico: PSE e PSE da sessão e variáveis bioquímicas (AGNEs, lactato, glicose e CK). Para as variáveis cardiovasculares, os resultados exibem uma diferença significativa na PAM (p=0,004) e PAD (p=0,02) de repouso, o mesmo não foi observado para PAS e FC de repouso e máxima. A suplementação com Ang-(1-7) não melhorou o desempenho físico de atletas de MTB em um protocolo de contrarrelógio.
Lifetime overproduction of circulating angiotensin‑(1‑7) in rats attenuates the increase in skeletal muscle damage biomarkers after exhaustive exercise.
(2019) Oliveira, Lenice Kappes Becker; Totou, Nádia Lúcia; Oliveira, Mariana Flávia; Coelho, Daniel Barbosa; Oliveira, Emerson Cruz de; Santos, Daisy Motta; Garcia, Emerson Silami; Santos, Maria José Campagnole dos; Santos, Robson Augusto Souza dos
Angiotensin‑(1‑7) (Ang‑[1‑7]) can modulate glucose metabolism and protect against muscular damage. The aim of this study was to investigate the influence of lifetime increase of circulating levels of Ang‑(1‑7) at exhaustive swimming exercise (ESE). Sprague‑Dawley (SD) and transgenic rats TGR(A1‑7)3292 (TR) which overproduce Ang‑(1‑7) (2.5‑fold increase) were submitted to ESE. The data showed no differences in time to exhaustion (SD: 4.90 ± 1.37 h vs. TR: 5.15 ± 1.15 h), creatine kinase, and transforming growth factor beta (TGF-β). Lactate dehydrogenase (SD: 219.9 ± 12.04 U/L vs. TR: 143.9 ± 35.21 U/L) and α‑actinin (SD: 336.7 ± 104.5 U/L vs. TR: 224.6 ± 82.45 U/L) values were significantly lower in TR. There was a significant decrease in the range of blood glucose levels (SD: −41.4 ± 28.32 mg/dl vs. TR: −13.08 ± 39.63 mg/dl) in SD rats. Muscle (SD: 0.06 ± 0.02 mg/g vs. TR: 0.13 ± 0.01 mg/g) and hepatic glycogen (SD: 0.66 ± 0.36 mg/g vs. TG: 2.24 ± 1.85 mg/g) in TR were higher. The TR presented attenuation of the increase in skeletal muscle damage biomarkers and of the changes in glucose metabolism after ESE.
Oral formulation angiotensin-(1-7) therapy attenuates pulmonary and systemics damage in mice with emphysema induced by elastase.
(2020) Bastos, Aline Cândida; Magalhães, Giselle Santos; Gregório, Juliana Fabiana; Matos, Natália Alves de; Santos, Daisy Motta; Bezerra, Frank Silva; Santos, Robson Augusto Souza dos; Santos, Maria José Campagnole dos; Machado, Maria da Glória Rodrigues
Angiotensin-(1-7) [Ang-(1-7)], a peptide of the renin-angiotensin system, has anti-inflammatory, anti-fibrotic and antiproliferative effects in acute or chronic inflammatory disease of respiratory system. In this study, we evaluated the effect of treatment with Ang-(1-7) on pulmonary tissue damage and behavior of mice submitted to experimental model of elastase-induced pulmonary emphysema (PE). Initially, male C57BL/6 mice were randomly assigned into two main groups: control (CTRL) and PE. In the PE group, the animals received three intratracheal instillations of pancreatic porcine elastase (PPE) at 1-week intervals (0.2 IU in 50 μL of saline). The CTRL group received the same volume of saline solution (50 μL). Twenty-four hours after the last instillation, animals of the PE group were randomly divided into two groups: PE and PE + Ang-(1-7). The PE + Ang-(1-7) group was treated with 60 μg/kg of Ang-(1-7) and 92 μg kg of HPβCD in gavage distilled water, 100 μl. The CTRL and PE groups were treated with vehicle (HPβCD- 92 μg/kg in distilled water per gavage, 100 μl), orally daily for 3 weeks. On the 19th day of treatment, all groups were tested in relation to locomotor activity and exploratory behavior. After 48 h, the animals were euthanized and lungs were collected. The animals of PE group presented rupture of alveolar walls and consequently reduction of alveolar tissue area. Treatment with Ang-(1-7) partially restored the alveolar tissue area. The PE reduced the locomotor activity and the exploratory behavior of the mice in relation to the control group. Treatment with Ang-(1-7) attenuated this change. In addition, it was observed that Ang-(1-7) reduced lung levels of IL-1β and increased levels of IL-10. These results show an anti-inflammatory effect of Ang-(1-7), inducing the return of pulmonary homeostasis and attenuation of the behavioral changes in experimental model of PE by elastase.
The ACE2/Angiotensin-(1-7)/MAS axis of the renin-angiotensin system : focus on Angiotensin-(1-7).
(2018) Santos, Robson Augusto Souza dos; Sampaio, Walkyria Oliveira; Alzamora, Andréia Carvalho; Santos, Daisy Motta; Alenina, Natalia; Bader, Michael; Santos, Maria José Campagnole dos
The renin-angiotensin system (RAS) is a key player in the control of the cardiovascular system and hydroelectrolyte balance, with an influence on organs and functions throughout the body. The classical view of this system saw it as a sequence of many enzymatic steps that culminate in the production of a single biologically active metabolite, the octapeptide angiotensin (ANG) II, by the angiotensin converting enzyme (ACE). The past two decades have revealed new functions for some of the intermediate products, beyond their roles as substrates along the classical route. They may be processed in alternative ways by enzymes such as the ACE homolog ACE2. One effect is to establish a second axis through ACE2/ANG-(1–7)/MAS, whose end point is the metabolite ANG- (1–7). ACE2 and other enzymes can form ANG-(1–7) directly or indirectly from either the decapeptide ANG I or from ANG II. In many cases, this second axis appears to counteract or modulate the effects of the classical axis. ANG-(1–7) itself acts on the receptor MAS to influence a range of mechanisms in the heart, kidney, brain, and other tissues. This review highlights the current knowledge about the roles of ANG-(1–7) in physiology and disease, with particular emphasis on the brain.
Thermoregulatory responses, heart rate, and the susceptibility to anxiety in obese animals subjected to stress.
(2023) Santos, Áquila Rodrigues Costa; Abreu, Aline Rezende Ribeiro de; Noronha, Sylvana Izaura Salyba Rendeiro de; Reis, Thayane Oliveira; Santos, Daisy Motta; Chianca Júnior, Deoclécio Alves; Silva Junior, Luiz Gonzaga da; Menezes, Rodrigo Cunha Alvim de; Rodrigues, Cibele Velloso
Obesity and stress are related to cardiovascular diseases. Rats fed a high-fat diet (HFD) show increased cardiovascular reactivity to emotional stress and altered defensive behavioral responses. Indeed, changes in thermoregulatory responses in an aversive environment are observed in these animals. However, studies aimed at clarifying the physiological mechanisms linking obesity, stress hyperreactivity and behavioral changes are needed. The aim of this study was to evaluate the changes in thermoregulatory responses, heart rate, and the susceptibility to anxiety in obese animals subjected to stress. Nine-week high-fat diet protocol was effective in inducing obesity by increasing weight gain, fat mass, adiposity index, white epididymal, retroperitoneal, inguinal and brown adipose tissue. Animals induced to obesity and subjected to stress (HFDS group) by the intruder animal method showed increases in heart rate (HR), core body temperature and tail temperature. HFDS showed an increase in the first exposure to the closed arm (anxiety-like behavior) in elevated T-Maze (ETM). The groups did not differ with respect to panic behavior assessed in the ETM and locomotor activity in the open field test. Our study shows that HFDS animals presented increased reactivity to stress with higher stress hyperthermia and anxious behavior. Thus, our results present relevant information regarding stress responsiveness and behavioral changes in obese animals.