Navegando por Autor "Santoro, Marcelo Matos"

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    A new extraction and purification methodology of Bowman-Birk inhibitors from seeds and germinated seeds of Macrotyloma axillare.
    (2008) César, Jorgino Julio; Santana, Marcos Aurélio de; Oliveira, Marcelo Eustáquio; Santos, Alexandre Gonçalves; Miranda, Alexandre Amadeu Cerqueira de; Santos, Alexandre Martins Costa; Santoro, Marcelo Matos; Andrade, Milton Hércules Guerra de
    The Macrotyloma axillare Bowman-Birk inhibitors from seeds and germinated seeds (120 h) were purified by an alternative methodology and revealed the presence of different contents of inhibitors for each treatment. Mass analyses of inhibitors, from seeds and germinated seeds, showed masses of about 7 and 8 kDa. The 120 h-germinated seed fractions presented higher activity isoforms than seeds without treatment.
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    A novel and efficient and low-cost methodology for purification of Macrotyloma axillare (Leguminosae) seed lectin.
    (2008) Santana, Marcos Aurélio de; Santos, Alexandre Martins Costa; Oliveira, Marcelo Eurípedes; Oliveira, Jamil Silvano de; Babá, Élio Hideo; Santoro, Marcelo Matos; Andrade, Milton Hércules Guerra de
    The N-acetyl-galactosamine specific lectin fromMacrotyloma axillare seeds (LMA)was purified by precipitation and ion exchange chromatography. The LMA 0.2 mol L−1 fraction showed hemagglutinating activity on erythrocytes A1. The results for molecular mass determinations were about 28 kDa. The LMA pHdependent assays showed best hemagglutinating activity at pH 6.0–8.0; being decreased at acidic/alkaline conditions and by EDTA treatment. LMA is a tetramer at pH 8.2 and a dimer at pH 4.0. Human erythrocytes from ABO system confirmed the A1 specificity for LMA. This new methodology is useful and easy, with low costs, for lectin purification in large amounts.