Navegando por Autor "Saliba, Juliana Wilke"
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Item Laboratorial algorithm for serological diagnosis of visceral leishmaniasis using rK39-ICT, DAT-LPC and FC-Simplex IgG1.(2020) Vale, Isabela Natália Pascoal Campos do; Saliba, Juliana Wilke; Fonseca, Giuliana Schmidt França; Pascoal, Vanessa Peruhype Magalhães; Araújo, Fernanda Fortes de; Xavier, Marcelo Antônio Pascoal; Carvalho, Andréa Teixeira de; Campos, Fernanda Magalhães Freire; Andrade, Mariléia Chaves; Lula, Jamille Fernandes; Reis, Alexandre Barbosa; Lemos, Elenice Moreira; Carvalho, Sílvio Fernando Guimarães de; Oliveira, Edward; Martins Filho, Olindo AssisThe performance of distinct serological tests (rK39-ICT, IFAT, DAT-LPC, FC-Simplex IgG1) was assessed and a laboratorial algorithm was proposed for accurate diagnosis of VL. DAT-LPC and FC-Simplex IgG1 showed outstanding accuracy (AUC = 0.93) to identify VL patients. The use of a sequential serological algorithm (rK39-ICT screening followed by DAT-LPC or FC-Simplex IgG1) improved the global accuracy for VL (97.2%) diagnosis. An alternative approach for diagnosis of VL has been also assessed for interchangeable use of serum/whole blood lysate samples in DAT-LPC and FC-Simplex IgG1. Our data showed an outstanding agreement for the results obtained with whole blood lysate samples as compared to serum samples (DAT-LPC =100%; FC-Simplex IgG1 = 99%). Together, these findings provide insights to improve the current overall accuracy of VL diagnosis and present innovative laboratorial tests and alternative samples from use in public health services.