Navegando por Autor "Petroianu, Andy"
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Item Changes in splenic uptake pattern associated with X-ray irradiation.(2020) Faria, Fernando Pereira de; Petroianu, Andy; Campos, Paula Peixoto; Lazari, Marcela Guimarães Takahashi de; Geraldo, Jony Marques; Nascimento, Clara Bezerra do; Siqueira, Sávio LanaPurpose: To evaluate the splenic uptake function after irradiation with high-energy X-rays. Materials and methods: Fourteen male Wistar rats were distributed into three groups. Group 1 (n ¼ 6) – control, non-irradiated; Group 2 (n ¼ 4) – animals that were irradiated and studied 24 h after irradiation; and Group 3 (n ¼ 4) – animals that were irradiated and studied 48 h after irradiation. The animals were irradiated with 8 Gy X rays in the abdominal region. According with the groups, after 24 or 48 h, 1 ml/kg of a 50% colloidal carbon solution was injected in the left internal jugular vein. After 40 min, the spleens were removed for histological studies. Macrophages containing carbon pigments in their cytoplasms were counted in 16 consecutive micro scopic fields, and their means were considered as the uptake pattern of each animal. Results: In the control groups, carbon pigments were captured by macrophages in the red and white pulps, while in the irradiated groups, the uptake in the marginal zone, around the white pulp, was enhanced. There was no disorder on the splenic parenchyma or necrosis in histological analyzes. Qualitatively rare apoptotic events were observed, with no difference between control and irradiated animals. Conclusion: The high-energy X-ray, used in radiotherapy, modifies the splenic clearance, enhancing the amount of marginal zone macrophages containing colloid particles. This radiation was not associated with morphological changes, nor with necrosis or apoptosis of splenic tissue.Item Neuroendocrine apendicopathy in morphologically normal appendices of patients with diagnosis of acute appendicitis : diagnostic study.(2020) Petroianu, Andy; Barroso, Thiago Vinicius Villar; Buzelin, Marcelo Araújo; Theobaldo, Bárbara de Melo; Tafuri, Luciene Simões de AssisBackground: About 15%–25% of appendices removed to treat acute appendicitis present normal macro- and macroscopic morphology. The objective of this study was to verify an association of proinflammatory, neuro endocrine and immune mediators with morphologically normal appendices removed from patients with clinical laboratorial and imaging characteristics of acute appendicitis. Materials and methods: Appendices removed from 121 adult patients of both genders were distributed into three groups according to their following characteristics: group 1: 53 macro- and microscopically normal appendices from patients with clinical, laboratorial and imaging diagnosis of acute appendicitis; group 2: 24 inflamed appendices from patients with clinical, laboratorial, imaging and histopathological diagnosis of acute appendi citis; group 3: 44 normal appendices from patients submitted to right colectomy to treat localized ascending colon adenocarcinoma. All appendices were immunohistochemically studied for gastrin inhibitor peptide, mast cell tryptase, vascular endothelial growth factor; intestinal vasoactive peptide, tumor necrosis factor alpha, interleukin 1, prostaglandin E2, gene-protein product 9.5, CD8 T lymphocytes, synaptophysine, enolase, and S100 protein. Results: The group 1 revealed increased levels of synaptophysine, enolase, mast cell tryptase and PGP-9.5 comparing with the other two groups. The group 2 presented increased levels of interleukin 1, CD8 T lympho cytes and prostaglandin E2 comparing with the other two groups. The group 3 confirmed the normal levels of all these neuroendocrine, immune and proinflammatory mediators. Conclusions: Morphologically normal appendices removed from patients with clinical and complementary exams indicating acute appendicitis have appendicular neuroimmunoendocrine disorder associated with the mediators synaptophysin, enolase, mast cell-related tryptase and gene-protein product 9.5.Item Neuroimmunoendocrine peptides on inflammed and morphologically normal appendices removed due to clinical acute appendicitis.(2019) Barroso, Thiago Vinicius Villar; Petroianu, AndyIntroduction: Despite clinical characteristics and complementary exams indicate acute appendicitis, 15% to 40% of all appendectomies result in removal of appendices with normal macro- and micromorphological aspects. Even so, manifestations of acute abdomen disappear immediately after the appendectomy, and never show back again. Objective: To assess changes of neuroimmunoendocrine peptides on removed appendices due to clinical presentation of acute appendicitis. Method: This article presents an updated revision of acute appendicitis, based on references found on PUBMED, LILACS, MEDLINE, WHOLIS and SciELO, using key words “acute appendicitis”, “neuroimmune appendicitis”, “neurogenic appendicopathy”, and “incidental appendectomy”. Results:Fourteen neuropeptides were analyzed by different authors who suggested the presence of neurogenic appendicopathy in morphologically normal appendices removed from patients with clinical presentation suggesting acute appendicitis. Conclusion: The etiopathogeny of acute appendicitis continues to be unknown, and there is a great possibility that patients with morphologically normal appendices with clinical presentation of acute appendicitis that heal after appendectomy present a neuroimmunoendocrine disease.Item Pathophysiology of acute appendicitis.(2016) Petroianu, Andy; Barroso, Thiago Vinicius VillarBackground: Acute appendicitis, the most common abdominal emergency that requires surgical treatment, shows a lifetime risk of 7%. Its overall incidence is approximately 11 cases per 10,000 individuals per year, and may occur at any age, although it is relatively rare at the extremes of age. Method: This article presents a recent-year-review of acute appendicitis, based on a study of references found in the PUBMED, using the key word of “pathophysiology of acute appendicitis” as research limitation. Results: The function of the appendix is not clearly understood, although the presence of lymphatic tissue on it suggests a role in the immune system. The primary pathogenic event in most of patients with acute appendicitis is believed to be due to luminal obstruction. Although being logical and likely to be true, this theory has not been proven. There is strong epidemiologic evidence supporting the proposition that perforated and non-perforated appendicitis are separate entities with a different pathogenesis. Recently, with the advent of neurogastroenterology, the concept of neuroimmune appendicitis has evolved. Considering that neurogenic disease may not include inflammatory signs, the name “neurogenic appendicopathy” seems to be a more appropriate term for appendices of morphological normal aspect in patients with clinical symptoms of acute appendicitis. Conclusion: As it can be perceived, based on the large number of studies related to acute appendicitis, it is not yet established the pathophysiology of this disease More research is in need to understand this still mysterious disease.