Navegando por Autor "Pessoa, Claudia do Ó"
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Item Cytotoxic potential of 14 Passiflora species against cancer cells.(2019) Amaral, Ricardo Guimarães; Gomes, Silvana Vieira Floresta; Luciano, Maria Claudia dos Santos; Pessoa, Claudia do Ó; Andrade, Luciana Nalone; Severino, Patrícia; Brandão, Geraldo Célio; Bomfim, Larissa Mendes; Soares, Milena Botelho Pereira; Bezerra, Daniel Pereira; David, Jorge Mauricio; Carvalho, Adriana AndradeThis work aimed to evaluate the cytotoxic potential against cancer cells of Passiflora genus plant species cultivated in Brazil and identify the mechanism of cytotoxicity induced by the most promising extract. Ethanolic extracts from the leaves of 14 Passiflora species were obtained by accelerated solvent extraction and in vitro cytotoxicity evaluated against cancer cell lines using MTT assay at a single concentration of 50 μg/ml. Additionally, the IC50 of the Passiflora alata (ELPA) leaf extracts was determined against both cancer (HCT-116, SF-295, OVACAR-8, and HL-60), and non-cancer cells (PBMC). The ELPA flavonoids were identified by HPLC-DAD and UHPLC-MS/MS. The morphological analyses, using light and fluorescence microscopy, and cell cycle and DNA fragmentation analysis, using flow cytometry, were evaluated to study the mechanism of cell death induced by ELPA in HL-60 cells. Among the Passiflora leaf extracts evaluated; ELPA stood out with high cytotoxic activity, followed by Passiflora capsularis and Passiflora quadrangularis with varying high and low cytotoxic activity. ELPA presented high cytotoxic potency in HL-60 (IC50 19.37 μg/ml), and without cytotoxicity against PBMC, suggesting selectivity for cancer cells. The cytotoxic activity of ELPA may well be linked to the presence of ten identified flavonoids. Cells treated with ELPA presented the hallmarks typical of apoptosis and necrosis, with cell cycle arrest in the G2/M phase. From among the studied species, ELPA presented greater cytotoxic activity, possibly a consequence of synergistic flavonoid action, which induces cell death by apoptosis and necrosis.Item Flavonoids, antioxidant, and antiproliferative activities of Stevia urticifolia.(2017) Machado, Kamilla Nunes; Hernández Tasco, Alvaro José; Salvador, Marcos José; Rodrigues, Ivanildes Vasconcelos; Pessoa, Claudia do Ó; Sousa, Ian Jhemes Oliveira; Ferreira, Paulo Michel Pinheiro; Nascimento, Andréa Mendes doItem In vitro activity evaluation of seven Brazilian Asteraceae against cancer cells and Leishmania amazonensis.(2019) Silva, Ana Cláudia Nogueira da; Nascimento, Renato Malveira Carreiro do; Rodrigues, Débora Caroline do Nascimento; Ferreira, Paulo Michel Pinheiro; Pessoa, Claudia do Ó; Lima, Daisy Jereissati Barbosa; Moraes Filho, Manoel Odorico de; Almeida, Raquel Martins de; Ferreira, Sebastião Rodrigo; Fujiwara, Ricardo Toshio; Nascimento, Andréa Mendes doNatural products are a very productive leading source of compounds for the development of medicines. The aim of this research was to investigate the in vitro antiproliferative and antileishmanial activities of 21 extracts from 7 Asteraceae species. Dried aerial parts of Asteraceae plant species were extracted using organic solvents in order of increasing polarities. Antiproliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, and nine extracts (43%) displayed moderate to high in vitro antiproliferative activity against human cancer cell lines HCT-116, OVCAR-8 and SF-295. Two crude extracts displaying antiproliferative activity were fractionated and yielded a fraction rich in lupeol acetate, a fraction rich in lupeol and 5,7,3′,4′-tetrahydroxyflavone-7-O-β-D-glucopyranoside. The antileishmanial activity was evaluated by amastigotes growth inhibition test in Leishmania amazonensis, and three extracts showed promising activity. Phytochemical screening of all plant extracts was also made for terpenoids, flavonoids, tannins, alkaloids and saponins.