Navegando por Autor "Pessoa, Cláudia"
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Item Bioactive extracts and chemical constituents of two endophytic strains of Fusarium oxysporum.(2012) Nascimento, Andréa Mendes do; Conti, Raphael; Turatti, Izabel Cristina Casanova; Cavalcanti, Bruno Coêlho; Lotufo, Leticia Veras Costa; Pessoa, Cláudia; Moraes Filho, Manoel Odorico de; Manfrim, Viviane; Toledo, Juliano Simões de; Cruz, Angela Kaysel; Pupo, Mônica TallaricoEthyl acetate extracts of cultures grown in liquid Czapek and on solid rice media of the fungal endophyte Fusarium oxysporum SS46 isolated from the medicinal plant Smallanthus sonchifolius (Poepp.) H. Rob., Asteraceae, exhibited considerable cytotoxic activity when tested in vitro against human cancer cells. Chromatographic separation yielded anhydrofusarubin (1) and beauvericin (2) that were identified based on their 1H and 13C NMR data. Compounds 1 and 2 showed the strongest cytotoxic activity against different cancer cell lines. Compound 2 also showed promising activity against Leishmania braziliensis. Hexanic extract of F. oxysporum SS50 grown on solid rice media also afforded a mixture of compounds that displayed cytotoxic activity against different cancer cell lines. Chemical analysis of the mixture of compounds, investigated by gas chromatography-mass spectrometry (GC-MS), showed that there was a predominance of methyl esters of fatty acids and alkanes.Item Biological screening of extracts of Brazilian Asteraceae plants.(2013) Carvalho, Cintia Cristina de; Machado, Kamilla Nunes; Ferreira, Paulo Michel Pinheiro; Pessoa, Cláudia; Fonseca, Thaisa Helena Silva; Gomes, Maria Aparecida; Nascimento, Andréa Mendes doNatural products are a very productive source of leads for the development of medicines. Seven Brazilian Asteraceae adult plants were randomly chosen. The current study was designed to evaluate the antiprotozoal and cytotoxic activities in vitro of 21 extracts obtained. Phytochemical properties of the most active extracts also were checked. Cytotoxic activity was evaluated by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) method against human tumor cell lines (HCT-116, OVCAR 8 and SF-295). The antiprotozoal activity was evaluated against Trichomonas vaginalis, Entamoeba histolytica and Giardia lamblia. None of the extracts showed antiprotozoal activity. However, 76% of the extracts displayed moderate to high in vitro cytotoxic activities against human cancer cell which suggests that they are a promising source of anticancer compound, since none of the extracts showed hemolytic activity. Terpenoids, flavonoids, saponins, tannins, besides other compound classes, were identified and may be responsible for their antitumor activity. Cytotoxic assays indicate the anticancer potential of Asteraceae species from Brazil.