Navegando por Autor "Oliveira, Luiza Barbosa de"
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Item Buccal micronucleus cytome assay : inter-laboratory scoring exercise and micronucleus and nuclear abnormalities frequencies in different populations from Brazil.(2020) Rohr, Paula; Silva, Gabrieli Flesch da; Vicentini, Veronica Elisa Pimenta; Almeida, Igor Vivian de; Santos, Raquel Alves dos; Takahashi, Catarina Satie; Goulart, Mirian Oliveira; Silva, Glenda Nicioli da; Oliveira, Luiza Barbosa de; Grisolia, Cesar Koppe; Piau, Tathyana Benetis; Branco, Carmen Lucia Bassi; Reis, Érica de Melo; Galvão, Marcos Felipe de Oliveira; Medeiros, Silvia Regina Batistuzzo de; Monteiro, Magaly Sales; Lopes, Reynaldo Assis de Vasconcelos; Brandão, Sabrina Fuziger Inácio; Batista, Nelson Jorge Carvalho; Paz, Márcia Fernanda Correia Jardim; Alencar, Juliana da Silva AraújoThe Buccal Micronucleus Cytome Assay (BMCyt) has become an important biomonitoring tool for assessing cytogenetic damage in many studied populations. Each laboratory applies protocols that vary according to the method of collecting and preparing samples. Besides, Brazil is a country of great territorial extensions that received immigrants from various parts of the world with different genetic backgrounds. Therefore, the present study aimed to evaluate the inter-laboratory variation in scoring the same set of slides using the more comprehensive scoring criteria, to standardize the BMCyt protocol, to observe the basal alterations in populations of different Brazilian regions and to compare it with other places around the world. Our results showed that a valuable number of laboratories participated, ten laboratories from different regions of the country, for the validation of the BMCyt in human biomonitoring studies, resulting in the 804 healthy individuals. This was possible because we observed: a range of measures needs to be considered, such as the baseline frequency of DNA damage and cell death in non-exposed individuals; age when grouped showed an influence on DNA damage, although when evaluated by group we did not see an influence; association between smoking habit and all endpoints of the BMCyt (except karyolytic cells) was evident; the basal MN frequency, in the majority of groups, follows those around the world; and the BMCyt was confirmed as a good health status biomarker. We emphasize the need for constant discussions on the parameters of cell death due to greater difficulty among the analyzers.Item Increase of binucleated cells in the oral mucosa : a study on the use of psychotropics by students of a Brazilian institution.(2019) Oliveira, Luiza Barbosa de; Parreiras, Joyce Aparecida Rezende; Sebastião, Elza Conceição de Oliveira; Silva, Glenda Nicioli daThis study aimed to assess the prevalence of medical and nonmedical use of psychiatric medication among undergraduate students of health sciences from a public university in Brasil. Another objective was to determine the frequency of nuclear morphological abnormalities in the buccal mucosa of students using psychiatric drugs. A cross-sectional study based on a Web survey was carried out with 375 health sciences undergraduate students from schools of Pharmacy, Physical Education, Nutrition, and Medicine. Additionally, spontaneous genetic damages in exfoliated cells of the buccal mucosa of 41 individuals by counting micronucleus (MN) and binucleated (BN) cells frequencies were evaluated. The results showed 76 (20.3%) of students reported the use of psychotropic drugs after enrolling in university. The majority of these students were from Pharmacy and Medicine programs, females, aged between 18-25 years old, nonsmokers, alcohol addicts, and with a family history of mental illness. In addition, Medical students, individuals with high-income, who live alone and are in the last period of the program are more likely to use psychotropic drugs. Moreover, exposure to psychiatric medication was able to increase the number of binucleated cells. These results provide evidence that the use of psychoactive drugs is increased in the academic context and may be related to the failure of the cell cycle.Item Treatment of keloids : a literature review.(2019) Corrêa, Pedro Martins; Passos, Camila Eugênia Fonseca; Souza, Eduardo Mesquita de; Batista, Guilherme Augusto Sousa; Jacintho, Jessica de Oliveira; Oliveira, Luiza Barbosa de; Lisboa, Oscar Campos; Lima, Joyce de Sousa FioriniIntroduction: Keloids are characterized by an abnormal response to dermal trauma, resulting in fibroblast proliferation, excessive collagen production, and impairment of adjacent healthy tissue. The diagnosis is clinical, and many conservative and surgical methods can be used as treatments. However, data on the efficacy of these treatments are limited, and there is no consensus regarding the best treatment option. This gap needs to be filled by developing comprehensive evidence-based therapies. Methods: A non-systematic literature review of keloid scars was carried out using PubMed, Scielo, MEDLINE, UptoDate, and dermatology and dermatological surgery textbooks. Literature review: The search retrieved relevant information on surgical and adjuvant therapies used for keloids, including excision, intralesional injections, cryotherapy, laser therapy, silicone gel sheeting, radiation therapy, and pressure therapy. These data are crucial because, in addition to complaints of pain, itching, and restriction of movement, the main reason for seeking treatment for keloids is for cosmetic and aesthetic improvement, and the rates of recurrence and treatment failure are high, emphasizing the importance of creating awareness regarding the available procedures and their effectiveness. Conclusion: Many surgical and adjuvant therapies for keloids are available. Nonetheless, there is no consensus on a universally accepted treatment. Therefore, additional high-quality studies are needed to identify the most effective therapeutic approaches to achieve better results.