Navegando por Autor "Oliveira, Fábio Faria"

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Draft genome sequence of Wickerhamomyces anomalus LBCM1105, isolated from cachaça fermentation.
(2020) Cunha, Aureliano Claret da; Santos, Renato Augusto Corrêa dos; Pachón, Diego Mauricio Riaño; Squina, Fábio Márcio; Oliveira, Juliana Velasco de Castro; Goldman, Gustavo Henrique; Souza, Aline Tieppo de; Gomes, Lorena Soares; Santos, Fernanda Godoy; Teixeira, Janaina Aparecida; Oliveira, Fábio Faria; Cruz, Izinara Rosse da; Castro, Ieso de Miranda; Lucas, Cândida; Brandão, Rogélio Lopes
Wickerhamomyces anomalus LBCM1105 is a yeast isolated from cachaça distillery fermentation vats, notable for exceptional glycerol consumption ability. We report its draft genome with 20.5x in-depth coverage and around 90% extension and completeness. It harbors the sequences of proteins involved in glycerol transport and metabolism.
High‑affinity transport, cyanide‑resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus.
(2019) Cunha, Aureliano Claret da; Gomes, Lorena Soares; Santos, Fernanda Godoy; Oliveira, Fábio Faria; Teixeira, Janaina Aparecida; Sampaio, Geraldo Magela Santos; Trópia, Maria José Magalhães; Castro, Ieso de Miranda; Lucas, Cândida Manuel Ribeiro Simões; Brandão, Rogélio Lopes
Wickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H+ symporter with twice the affinity of S. cerevisiae was identified. As in this yeast, WaSTL1 is repressed by glucose and derepressed/induced by glycerol but much more highly expressed. Moreover, LBCM1105 aerobically growing on glycerol was found to produce ethanol, providing a redox escape to compensate the redox imbalance at the level of cyanide-resistant respiration (CRR) and glycerol 3P shuttle. This work is critical for understanding the utilization of glycerol by non-Saccharomyces yeasts being indispensable to consider their industrial application feeding on biodiesel residue.
Lpx1p links glucose-induced calcium signaling and plasma membrane H+-ATPase activation in Saccharomyces cerevisiae cells.
(2017) Castanheira, Diogo Dias; Santana, Eduardo Perovano; Santos, Fernanda Godoy; Diniz, Raphael Hermano Santos; Oliveira, Fábio Faria; Pereira, Renata Rebeca; Trópia, Maria José Magalhães; Castro, Ieso de Miranda; Brandão, Rogélio Lopes
In yeast, as in other eukaryotes, calcium plays an essential role in signaling transduction to regulate different processes. Many pieces of evidence suggest that glucose-induced activation of plasma membrane H+-ATPase, essential for yeast physiology, is related to calcium signaling. Until now, it was not identified any protein that could be regulated by calcium in this context. Lpx1p, a serine-protease that is also involved in the glucose-induced activation of the plasma membrane H+-ATPase activation, could be a candidate to respond to intracellular calcium signaling involved in this process. In this work, and by using different approaches, we showed many pieces of evidence suggesting that the requirement of calcium signaling for activation of the plasma membrane H+-ATPase is due to its requirement for activation of Lpx1p. According to the current model, activation of Lpx1p would cause hydrolysis of an acetylated tubulin that keeps the plasma membrane H+-ATPase in an inactive state. Therefore, after its activation, Lpx1p would hydrolyze the acetylated tubulin making the plasma membrane H+-ATPase phosphorylation accessible for at least one protein kinase.
Quality improvement and geographical indication of cachaça (Brazilian spirit) by using local selected yeast strains.
(2016) Barbosa, Edilene Alves; Souza, Magalhães Teixeira de; Diniz, Raphael Hermano Santos; Santos, Fernanda Godoy; Oliveira, Fábio Faria; Correa, Lygia Fátima da Mata; Alvarez, Florencia; Coutrim, Maurício Xavier; Afonso, Robson José de Cássia Franco; Castro, Ieso de Miranda; Brandão, Rogélio Lopes
Aims: In order to improve the quality and to create a biological basis for obtainment of the protected denomination of origin (PDO), indigenous yeast were isolated and characterized for use in Salinas city (the Brazilian region of quality cachac a production). Material and methods: Seven thousand and two hundred yeast colonies from 15 Salinas city distilleries were screened based on their fermentative behaviour and the physicochemical composition of cachac a. Molecular polymorphic analyses were performed to characterize these isolates. Results: Two Saccharomyces cerevisiae strains (nos. 678 and 680) showed appropriate characteristics to use in the cachac a production: low levels of acetaldehyde and methanol, and high ethyl lactate/ethyl acetate ratio respectively. They also presented polymorphic characteristics more closely related between themselves even when compared to other strains from Salinas. Conclusions: The application of selected yeast to cachac a production can contribute for the improvement of the quality product as well as be used as a natural marker for PDO. Significance and Impact of the Study: This study suggests that the use of selected yeast strains could contribute to obtain a cachac a similar to those produced traditionally, while getting wide acceptation in the market, yet presenting more homogeneous organoleptic characteristics, and thus contributing to the PDO implementation.