Navegando por Autor "Nicolato, Roney Luiz de Carvalho"
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Item Apolipoprotein e polymorphism in Brazilian dyslipidemic individuals : Ouro Preto study.(2007) Lana, Angelo Mendes; Pena, Geórgia das Graças; Freitas, Silvia Nascimento de; Lima, Angélica Alves; Nicolato, Roney Luiz de Carvalho; Nascimento Neto, Raimundo Marques do; Coelho, George Luiz Lins Machado; Freitas, Renata Nascimento deThe influence of apolipoprotein E alleles and genotypes on plasma lipid levels was determined in 185 individuals of mixed ethnicity living in Ouro Preto, Brazil. DNA was obtained from blood samples and the genotypes were determined by an RFLP-PCR procedure. The *3 allele was the most frequent (72%), followed by *4 (20%) and *2 (8%); *4 frequency was higher and *2 frequency was lower in the dyslipidemic group than in the normal control group. The *2 carriers presented lower LDL and total cholesterol levels compared to the *3 and *4 carriers. All six expected genotypes were observed in the individuals genotyped: E2/2 (2.1%), E4/4 (2.7%), E2/4 (3.7%), E2/3 (8.0%), E3/3 (53.3%), E3/4 (29.9%); no difference in genotype frequencies was found between the normal and dyslipidemic groups. Compared with *2, the presence of *3 increases more than two times the risk for dyslipidemia (OR = 2.31; P = 0.025; 95% CI = 1.06-5.06) and the presence of *4 increases it three times (OR = 3.31; P = 0.006; 95% CI = 1.36-8.04). The only significant effect of genotype was an increased risk for dyslipidemia in the *4 genotype carriers (E3/4 + E4/ 4) compared with the *2 genotype carriers (E2/2 + E2/3) with OR = 3.69 (95% CI = 1.25-10.88). The present study indicates that in the Ouro Preto admixed population the presence of APOE *2 can confer a protective effect, whereas the presence of APOE *4 implies an enhanced risk for dyslipidemia.Item Avaliação da incidência de hemoglobinopatias em pacientes atendidos no laboratório piloto de análises clínicas da Escola de Farmácia – UFOP.(Programa de Pós-Graduação em Ciências Farmacêuticas. CIPHARMA, Escola de Farmácia, Universidade Federal de Ouro Preto., 2010) Nicolato, Roney Luiz de Carvalho; Carneiro, Cláudia Martins; Paula, Carmem Aparecida deAs hemoglobinopatias constituem um grupo de doenças de caráter genético caracterizadas pela síntese de cadeias polipeptídicas estruturalmente anormais ou diminuição da síntese de uma ou mais cadeias de globina. Dentre as variantes estruturais da hemoglobina, ou hemoglobinas anormais, as mais comuns são as hemoglobinas S e C, ambas resultantes da substituição de um único aminoácido na cadeia polipeptídica beta. Estudos realizados no Brasil mostram uma alta prevalência de heterozigotos para HbS e HbC. A frequência destas variantes na população brasileira é muito variável, pois está relacionada com os grupos raciais formadores de cada região. O povoamento da cidade de Ouro Preto, em 1698, motivado pela procura de ouro foi composto principalmente por portugueses e escravos africanos, contexto que favoreceu a mestiçagem entre os povos. Considerando que esses povos apresentam genes para as hemoglobinas anormais com frequências variadas, é esperado que se encontrem essas alterações genéticas na nossa população. O presente estudo teve como objetivo principal avaliar a frequência de hemoglobinas anormais nos pacientes atendidos pelo Laboratório Piloto de Análises Clínicas (LAPAC) da Escola de Farmácia da Universidade Federal de Ouro Preto (UFOP). No período de agosto de 2008 a setembro de 2010, foram triados 943 pacientes de ambos os sexos e diferentes faixas etárias para a frequência de variantes da hemoglobina, tendo como foco a hemoglobina falciforme, também conhecida como hemoglobina S. O diagnóstico foi realizado mediante a realização de eletroforese de hemoglobina em pH alcalino e as amostras positivas para alguma variante foram confirmadas por cromatografia líquida de baixa pressão (LPLC) e pelo teste de solubilidade. A prevalência de variantes estruturais de hemoglobina foi de 6,6%, sendo 4,88% para o traço falciforme (HbAS), 1,59 % para o genótipo AC e 0,11% para o genótipo SC. Não foram detectados indivíduos homozigotos para nenhum dos tipos de hemoglobinas variantes. Os resultados encontrados foram superiores aos encontrados nos estados de Pernambuco, Rio de Janeiro, Minas Gerais, São Paulo e Goiás, mostrando a dispersão dos genes para a HbS e HbC na população de Ouro Preto. Por essa razão, concluímos que é importante realizar programas com maior abrangência na população para estudo da epidemiologia das hemoglobinas variantes e de outras hemoglobinopatias resultantes de uma diminuição da síntese de uma ou mais cadeias de globina, no município de Ouro Preto.Item Cardiovascular risk factors in children and adolescents living in an urban area of southeast of Brazil : Ouro Preto study.(2009) Cândido, Ana Paula Carlos; Benedetto, Raquel; Castro, Ana Paula Pereira; Carmos, Joseane Souza do; Nicolato, Roney Luiz de Carvalho; Nascimento Neto, Raimundo Marques do; Freitas, Renata Nascimento de; Freitas, Silvia Nascimento de; Caiaffa, Waleska Teixeira; Coelho, George Luiz Lins MachadoThis study aims to identify risk factors for cardiovascular disorders in schoolchildren living in Ouro Preto City, Brazil. A cross-sectional study was carried out in a population-based sampling of schoolchildren (6– 14 years old), randomly selected and stratified by the proportion of students according to age and gender in each schools of the city. Biochemical, clinical and anthropometric variables as well as physical activity and family history were used in a logistic regression model for obesity or arterial hypertension. Out of 780 schoolchildren sampled, the risk of obesity was greater in subjects presenting high triglyceride and low high density lipoprotein-cholesterol levels, and those whose parents were obese, whilst the risk of hypertension was high in obese subjects and those who presented low birth weight. It was observed that 44.4% of the schoolchildren were exposed to two or three cardiovascular disease (CVD) risk factors and 8.2% were exposed to four or six factors. These findings should be considered in preventive measures to reduce the future risk for CVD among schoolchildren in Brazil.Item Cellular immunophenotypic profile in the splenic compartment during canine visceral leishmaniasis.(2014) Reis, Alexandre Barbosa; Carvalho, Andréa Teixeira de; Giunchetti, Rodolfo Cordeiro; Roatt, Bruno Mendes; Vital, Wendel Coura; Nicolato, Roney Luiz de Carvalho; Lemos, Denise da Silveira; Oliveira, Rodrigo Corrêa de; Martins Filho, Olindo AssisTo determine the role of the spleen in the pathogenesis of canine visceral leishmaniasis (CVL), we analyzed cellular immunophenotypic profiles of 52 dogs naturally infected with Leishmania infantum, clinically classified as follows: asymptomatic dogs-I (AD-I), seroneg-ative/PCR+; asymptomatic dogs-II (AD-II), seropositive/PCR+; oligosymptomatic dogs (OD) and symptomatic dogs (SD). Seven non-infected dogs (CD) were included as a control group. AD-II presented higher levels of CD8+ T splenocytes and lower TCD4+/TCD8+ ratio in com-parison with CD. OD and SD showed lower percentages of CD21+ as compared with AD-II. All seropositive dogs presented lower levels of CD45RA+ than CD. Regardless of the stimuli used, the proliferation index from splenocytes in vitro was inversely correlated with clini-cal status. After LSA stimulation, there was a higher percentage of specific CD8+ T in AD-II than CD and non-stimulated culture. In contrast, splenocytes from SD under in vitro LSA stimulation induced decreased MHC-II+ expression in comparison with all groups, and non-stimulated culture. In conclusion, the role of CD8+ T splenocytes seems to be important for an effective immunological response, a hallmark of asymptomatic CVL, whereas the pro-nounced loss of MHC-II expression upon LSA stimulation is a biomarker of symptomatic CVL.Item Clinical forms of canine visceral Leishmaniasis in naturally Leishmania infantum – infected dogs and related myelogram and hemogram changes.(2013) Nicolato, Roney Luiz de Carvalho; Abreu, Raquel Trópia de; Roatt, Bruno Mendes; Soares, Rodrigo Dian de Oliveira Aguiar; Reis, Levi Eduardo Soares; Carvalho, Maria das Graças; Carneiro, Cláudia Martins; Giunchetti, Rodolfo Cordeiro; Bouillet, Leoneide Érica Maduro; Lemos, Denise da Silveira; Vital, Wendel Coura; Reis, Alexandre BarbosaHematological analysis has limited applications for disease diagnosis in Leishmania infantum–infected dogs, but it can be very important in evaluating the clinical forms of the disease and in understanding the evolution of canine visceral leishmaniasis (CVL) pathogenesis. Recently, we demonstrated that alterations in leucopoiesis and erythropoiesis are related to clinical status and bone marrow parasite density in dogs naturally infected by L. infantum. To further characterize these alterations, we evaluated the association between the hematological parameters in bone marrow and peripheral blood alterations in groups of L. infantum–infected dogs: asymptomatic I (AD-I: serum negative/PCR+), asymptomatic II (AD-II: serum positive), oligosymptomatic (OD), and symptomatic (SD). Results were compared with those from noninfected dogs (NID). The SD group was found to present a decrease in erythropoietic lineage with concomitant reductions in erythrocytes, hemoglobin, and hematocrit parameters, resulting in anemia. The SD group also had increased neutrophils and precursors and decreased band eosinophils and eosinophils, leading to peripheral blood leucopenia. In the AD-II group, lymphocytosis occurred in both the peripheral blood and the bone marrow compartments. The SD group exhibited lymphocytosis in the bone marrow, with lymphopenia in the peripheral blood. In contrast, the AD-I group, showed no significant changes suggestive of CVL, presenting normal counts in bone marrow and peripheral blood. Our results showed for the first time that important changes in hematopoiesis and hematological parameters occur during ongoing CVL in naturally infected dogs, mainly in symptomatic disease. Taken together, our results based on myelogram and hemogram parameters enable better understanding of the pathogenesis of the anemia, lymphocytosis, and lymphopenia, as well as the leucopenia (eosinopenia and monocytopenia), that contribute to CVL prognosis.Item CXCL-16, IL-17, and bone morphogenetic protein 2 (BMP-2) are associated with overweight and obesity conditions in middle-aged and elderly women.(2017) Ribeiro, Silvana Mara Luz Turbino; Lopes, Laís Roquete; Costa, Guilherme de Paula; Figueiredo, Vivian Paulino; Bajracharya, Deena Shrestha; Batista, Aline Priscila; Nicolato, Roney Luiz de Carvalho; Oliveira, Fernando Luiz Pereira de; Estanislau, Juliana de Assis Silva Gomes; Silva, André Talvani Pedrosa daThe current concept of overweight/obesity is most likely related to a combination of increased caloric intake and decreased energy expenditure. Widespread inflammation, associated with both conditions, appears to contribute to the development of some obesity-related comorbidities. Interventions that directly or indirectly target individuals at high risk of developing obesity have been largely proposed because of the increasing number of overweight/obese cases worldwide. The aim of the present study was to assess CXCL16, IL-17, and BMP-2 plasma factors in middle-aged and elderly women and relate them to an overweight or obese status. In total, 117 women were selected and grouped as eutrophic, overweight, and obese, according to anthropometric parameters. Analyses of anthropometric and circulating biochemical parameters were followed by plasma immunoassays for CXCL-16, IL- 17, and BMP-2.Item Derivation and external validation of a simple prediction model for the diagnosis of type 2 Diabetes Mellitus in the Brazilian urban population.(2009) Sousa, André Gustavo Pires de; Pereira, Alexandre da Costa; Marquezine, Guilherme Figueiredo; Nascimento Neto, Raimundo Marques do; Freitas, Silvia Nascimento de; Nicolato, Roney Luiz de Carvalho; Coelho, George Luiz Lins Machado; Rodrigues, Sérgio Lamego; Mill, José Geraldo; Krieger, José EduardoA risk score model was developed based in a population of 1,224 individuals from the general population without known diabetes aging 35 years or more from an urban Brazilian population sample in order to select individuals who should be screened in subsequent testing and improve the efficacy of public health assurance. External validation was performed in a second, independent, population from a different city ascertained through a similar epidemiological protocol. The risk score was developed by multiple logistic regression and model performance and cutoff values were derived from a receiver operating characteristic curve. Model’s capacity of predicting fasting blood glucose levels was tested analyzing data from a 5-year follow-up protocol conducted in the general population. Items independently and significantly associated with diabetes were age, BMI and known hypertension. Sensitivity, specificity and proportion of further testing necessary for the best cutoff value were 75.9, 66.9 and 37.2%, respectively. External validation confirmed the model’s adequacy (AUC equal to 0.72). Finally, model score was also capable of predicting fasting blood glucose progression in non-diabetic individuals in a 5-year follow-up period. In conclusion, this simple diabetes risk score was able to identify individuals with an increased likelihood of having diabetes and it can be used to stratify subpopulations in which performing of subsequent tests is necessary and probably cost-effective.Item Estudo da maturação e das alterações eritrocitárias e leucocitárias em cães naturalmente infectados por Leishmania (Leishmania) infantum.(2014) Nicolato, Roney Luiz de Carvalho; Reis, Alexandre Barbosa; Carneiro, Cláudia MartinsAnálises hematológicas apresentam aplicações limitadas para o diagnóstico da doença em cães infectados com L. infantum, mas podem ser importantes para a avaliação das formas clínicas da doença e para a compreensão da evolução da patogênese leishmaniose visceral canina (LVC). Recentemente, foi demonstrado que alterações na leucopoese e eritropoese estão relacionadas com o estado clínico e densidade parasitária da medula óssea em cães naturalmente infectados por L. infantum. Para melhor caracterizar estas alterações, no presente estudo foi avaliada a associação entre os parâmetros hematológicos na medula óssea e alterações do sangue periférico em grupos de cães infectados por L. infantum: Cães assintomáticos I (CA-I: soro negativo/PCR+; n=6), assintomáticos II (CA- II: soro/PCR+; n=7), oligossintomáticos (CO; n=8), e cães sintomáticos (CS; n=12) e cães não infectados (CNI; n=7). A densidade parasitária foi avaliada na medula óssea e os resultados expressos como “Leishman Donovan Units” (LDU), e classificados em tercis como baixo (BP, n=7) ou alto (AP, n=7) parasitismo. No grupo CS foi encontrado uma diminuição na linhagem eritropoética com reduções concomitantes em eritrócitos circulantes, bem como nos parâmetros de hemoglobina e hematócrito, resultando em anemia. Neste mesmo grupo CS também observou-se aumento de precursores de neutrófilos e eosinófilos porém com diminuição de bastonetes neutrófilos e eosinófilos circulantes, levando a leucopenia no sangue periférico. No grupo CA-II, foi detectada uma linfocitose que ocorreu tanto no sangue periférico como no compartimento da medula óssea. O grupo CS exibiu linfocitose na medula óssea, com linfopenia no sangue periférico. Em contraste, o grupo CA- I, não apresentaram alterações significativas sugestivas de LVC, apresentando contagens normais tanto na medula óssea como no sangue periférico. A avaliação do impacto do parasitismo na medula óssea mostrou resultados similares quando se avaliou os cães classificados em diferentes formas clínicas, tais como, hipoplasia eritróide, hipoplasia eosinofílica e proliferação de células precursoras neutrofílica no grupo de cães com alto parasitismo. Os nossos resultados mostram pela primeira vez que importantes alterações na hematopoiese e parâmetros hematológicos (hemograma) podem ocorrer durante o curso de LVC em cães naturalmente infectados por L. infantum, principalmente na doença sintomática. Tomados em conjunto, os nossos resultados com base nos parâmetros do mielograma e hemograma permitem uma melhor compreensão da patogênese da anemia, linfocitose e linfopenia, bem como a leucopenia (eosinopenia e monocitopenia), que contribuem para o prognóstico e monitoração da LVC.Item Lipoprotein(a) as a risk factor associated with ischemic heart disease : Ouro Preto Study.(2007) Cândido, Ana Paula Carlos; Ferreira, Silvania; Lima, Angélica Alves; Nicolato, Roney Luiz de Carvalho; Freitas, Silvia Nascimento de; Brandão, Paulo Eduardo; Pereira, Alexandre da Costa; Krieger, José Eduardo; Nascimento Neto, Raimundo Marques do; Coelho, George Luiz Lins MachadoEvidences suggest that lipoprotein(a) [Lp(a)] is an important risk factor for cardiovascular disease. However, literature has been controversial in confirming its role as an independent risk factor for cardiovascular disease. The objective of the present study is to evaluate the association between serum levels of Lp(a) and ischemic heart disease as well as other cardiovascular risk factors in a population-based study conducted on a local cohort of the Brazilian population. Lp(a) serum levels were measured in 400 individuals selected from a larger sample of a populational survey carried out in Ouro Preto, a city in the southeast of Brazil. Lipid profile, fasting blood glucose, anthropometric and clinical parameters were analyzed. Lp(a) levels were significantly associated with the presence of ischemic heart disease. In relation to other cardiovascular risk factors, it was verified that Lp(a) levels were statistically associated with age, total cholesterol, LDL-cholesterol and percentage of body fat determined by bioelectric impedance. Lp(a) was also highly associated with the Framingham risk score (p = 0.003). In a multivariate analysis two significant interactions were revealed; one involving ischemic heart disease, sex and age and other associating ischemic heart disease, age and total cholesterol. In summary, in the present analysis Lp(a) serum levels were correlated with the occurrence of ischemic heart disease and other cardiovascular risk factors.Item Lipoprotein(a) levels in children and adolescents : Ouro Preto study.(2020) Cândido, Ana Paula Carlos; Mendes, Alekson Mendonça; Candido, Debora Rosa Carlos; Nicolato, Roney Luiz de Carvalho; Coelho, George Luiz Lins MachadoBackground: Lipoprotein (a) is a cardiovascular risk factor in adult. Studies have shown the presence of this emergent risk factor in school children, which may contribute to the development of atherosclerosis in adulthood. Objective: To evaluate the association between lipoprotein (a) and cardiovascular risk factors in school children. Methods: Lipoprotein (a) levels were measured in 320 school children (6-14 years) selected from a population survey carried out in Ouro Preto (southeast of Brazil). Demographic (sex and age), biochemical (total cholesterol, high density lipoprotein-cholesterol, low-density lipoprotein-cholesterol, triglycerides, and glucose), anthropometric (body mass index, waist circumference, body fat percentage), clinical (arterial blood pressure, pubertal stage and birth weight) and economic (family income) parameters, as well as family history (obese and/or hypertensive parents) were analyzed. Non-parametric analysis was used to evaluate lipoprotein (a) levels in each subgroup. Variables with p≤0.20 in the univariate analysis were included in binary regression logistic model. Differences with p < 0.05 were considered significant. Results: Lipoprotein (a) levels were associated with total cholesterol (p=0.04), body fat (p=0.009), and mother´s systolic (p=0.02) and diastolic blood pressure (p=0.04). In a logistic regression analysis, children with high lipoprotein (a) levels and body fat, and children born from hypertensive mothers were, respectively, at 3.2(p=0.01) and 1.4 (p=0.03) times higher risk than other children. In clustering these factors, elevated lipoprotein (a) was 2.6 times more likely to be seen in school children with high body fat and born hypertensive mothers. Conclusions: Lipoprotein (a) was correlated with cardiovascular risk factors in children and adolescents. Persistence of these risk factors in childhood suggests a contribution of elevated lipoprotein (a) to future cardiovascular disease.Item Retinol-binding protein 4 and insulin resistance are related to body fat in primary and secondary schoolchildren : the Ouro Preto study.(2014) Castro, Ana Paula Pereira; Cândido, Ana Paula Carlos; Nicolato, Roney Luiz de Carvalho; Caldas, Ivo Santana; Coelho, George Luiz Lins MachadoPurpose Evidence suggests that plasma retinol-binding protein 4 (RBP4) and insulin resistance are related to body fat (BF). We aimed to assess the relationship between RBP4 and insulin resistance with obesity in a mixed (skin color) cohort of the Brazilian population. Methods A nested case–control study was conducted in 227 schoolchildren aged 7–14 years. Schoolchildren with a high BF percentage (% BF, C 30 for girls and C 25 for boys) were identified as the obese group (n = 137), and those with lower values were identified as the non-obese group (n = 90). Percentage of body fat (% BF) was determined by tetrapolar bioimpedance (Quantum II, RJL System), RBP4 by enzyme-linked immunosorbent assay (Immunology Consultants Laboratory), plasma fasting insulin by chemiluminescent immunoassay (Access Immunoassay System) and insulin resistance by the homeostasis model insulin resistance (IRHOMA) index. Serum lipid profile and arterial blood pressure were evaluated. Results The significant independent risk factors associated with obesity were as follows: male sex, increased serum LDL-C, RBP4 and IRHOMA. Among children with higher RBP4, the association with obesity increased significantly (from 3.1 to 8.5) in the presence of insulin resistance, when compared to higher RBP4 and non-insulin resistance. Conclusion IRHOMA and RBP4 showed significant associations with obesity and traditional CVD risk factors. They might therefore be used as a marker for CVD risk and have clinical implications in the development of comorbidities associated with obesity.Item The overweight increases circulating inflammatory mediators commonly associated with obesity in young individuals.(2018) Lopes, Laís Roquete; Ribeiro, Silvana Mara Luz Turbino; Figueiredo, Vivian Paulino; Leite, Ana Luísa Junqueira; Nicolato, Roney Luiz de Carvalho; Estanislau, Juliana de Assis Silva Gomes; Oliveira, Fernando Luiz Pereira de; Silva, André Talvani Pedrosa daObesity is a serious and growing world healthy problem affecting developed and developing countries. The new conception of obesity as a basal inflammatory condition has opened a new window of possibilities to identify inflammatory biomarkers to be used in the diagnosis or prognosis of obesity-associated comorbidities. This present work aims the identification of the adipokines (leptin and resistin), chemokines (CCL2, CCL5, CXCL16) and the BMP-2 and their association with the clinical, biochemical (fasting glucose, hemogram, cholesterol, T3, T4 and TSH) and anthropometric (weight, height, body circumferences, skinfold thickness and percentage of body fat) parameters in young adults (18–30 years old) presenting obesity and overweight. Our data showed increasing in anthropometric parameters and in the plasma inflammatory levels in those individuals presenting overweight and obesity. We observed a higher plasma levels of CCL2, CCL5, CXCL16, leptin and resistin in those overweigh and obese individuals. In addition, the CCL2, CCL5 presented a positive correlation with the body mass index and the body fat percentage. Assuming the obesity as a systemic inflammatory process, in this current study, the overweight individuals possess a close similar pattern of circulating inflammatory mediators which might be a potential risk of the development of obesity comorbidities. Further studies are still needed to precise the role of the biomarkers CCL2, CCL5, CXCL16 and BMP-2 in the clinical prognosis related to the overweight or obese individuals.