Navegando por Autor "Machado, Francisco Paiva"
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Item Nanoemulsion of Ocotea indecora (Shott) Mez essential oil : larvicidal effects against Aedes aegypti .(2023) Machado, Francisco Paiva; Andrade, Diogo Folly Gomes; Salas Enríquez, Jairo Jhonatan; Mello Neto, Cícero Brasileiro de; Esteves, Ricardo dos Santos; Araújo, Raquel Silva; Toledo, Pedro Felipe Seguro de; Mantilla Afanador, Javier Guillermo; Santos, Marcelo Guerra; Oliveira, Eugênio Eduardo de; Ricci Junior, Eduardo; Rocha, Leandro MachadoThe widespread use of insecticide can lead to the resistance of the Aedes aegypti mosquito and adverse effects on non-target organisms, such as humans, other animals, and insects. In this sense, nanotechnology associated with natural products is a promising alternative to obtaining safer and more sustainable insecticide formulations against this vector. Therefore, in this research, we developed and optimized a nanoemulsion with essential oil from Ocotea indecora (Shott) Mez leaves and evaluated its larvicidal properties against Ae. aegypti larvae. In addition, oral toxicity assays were performed to test the nanoemulsion safety of the non-target organism Apis mellifera. The major constituent found was sesquirosefuran (81.4 %). The nanoemulsions were prepared by the low-energy method by phase inversion and characterized by the dynamic light scattering technique. The most suitable surfactant mixture was in hydrophilic-lipophilic balance 14.22, presenting droplets size of 122.8 nm and polydispersity index of 0.262. Then a 23 factorial design was realized to optimize the formulation suggesting the variables conditions of 1:1 of essential oil (5 % w/w) and surfactants at 500 rotations per minute. This led to spherical nanoemulsions with mean size and PdI of 105.3 nm and 0.263, respectively. The optimized nano- emulsion presented stability when stored at room temperature and refrigerated for up to one year. The LC50 values against Ae. aegypti larvae were 61.4, and 26.8 μg/mL after, 48, and 144 h, respectively. Scanning electron micrography showed morphological body alterations on the larvae Ae. aegypti treated with the nanoemulsion. Regarding the ecotoxicological evaluation, the nanoemulsion showed no toxicity against Apis mellifera. There- fore, this work demonstrated a simple method to obtain O. indecora nanoemulsion as an environmental-friendlier alternative to the Aedes aegypti control.Item Poly-ε-caprolactone nanocapsules loaded with copaiba essential oil reduce inflammation and pain in mice.(2023) Pinto, Erveton Pinheiro; Costa, Sarah Olivia Alves Mendes da; D’Haese, Cecile; Nysten, Bernard; Machado, Francisco Paiva; Rocha, Leandro Machado; Souza, Tiago Marcolino de; Beloqui, Ana; Machado, Renes Resende; Araújo, Raquel SilvaDiverse drugs have been used for the management of inflammation disorders and pain. However, they present many side effects and stimulate the search for new pharmacotherapeutic alternatives. Plant-derived products such as copaiba essential oil (CO) offer beneficial pharmacological effects. On the other hand, essential oil’s low water solubility and physical instability hinder its in vivo application. Thus, poly-ε-caprolactone (PCL)-based nanocarriers have been used to increase their stability and efficacy. This work aimed to encapsulate CO in PCL nanocapsules and evaluate their effect on inflammation models and pain. The polymeric nanocapsules loading CO (CO-NC) were prepared by nanoprecipitation technique, characterized, and analyzed for their anti- inflammatory effect in vitro and in vivo. The results showed that CO-NC presented a spherical shape, 229.3 ± 1.5 nm diameter, and a negative zeta potential (approximately − 23 mV). CO and CO-NC presented anti- inflammatory and antioxidant effects by LPS-activated macrophages (J774 cells). In addition, CO-NC signifi- cantly reduced TNF-α secretion (3-fold) compared to CO. In vivo, pre-treatment with CO or CO-NC (50, 100, 200 mg/kg, intraperitoneal; i.p) reduced the mechanical allodynia, paw edema, and pro-inflammatory cytokines induced by intraplantar (i.pl) injection of carrageenan in mice. Specifically, CO-NC (200 mg/kg; i.p.) reduced the production of TNF-α similar to the control group. Our results support using polymeric nanocapsules for CO delivery in inflammatory conditions.