Navegando por Autor "Lima, William Gustavo de"
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Item Evaluation of the activity of Tontelea micrantha extracts against Bacteria, Candida and Mayaro virus.(2018) Nizer, Waleska Stephanie da Cruz; Ferraz, Ariane Coelho; Lima, William Gustavo de; Moraes, Thaís de Fátima Silva; Ferreira, Fernanda Lopes; Ferreira, Jaqueline Maria Siqueira; Magalhães, Cíntia Lopes de Brito; Duarte, Lucienir Pains; Vieira Filho, Sidney Augusto; Magalhães, José Carlos deObjectives: This work aimed to evaluate the antibacterial, antifungal, and anti-Mayaro virus(MAYV) activity of leaf and branch extracts from Tontelea micrantha. Materials and Methods: T. micrantha extracts were prepared through the partition of the leaf and branch samples in different solvents. Then, the antibacterial and antifungal activity was assessed against bacterial pathogens and Candida sp. by the determination of the minimum inhibitory concentration (MIC) by the broth microdilution method. The activity against anti-MAYV was evaluated through the quantification of the extract concentration that promoted the protection of 50% of the cells after the viral infection. Results: The extracts of T. micrantha were inactive (MIC >500 µg/mL) against Gram-positive, Gram-negative and Candida species at the highest concentration tested (500 µg/mL). Anti-MAYV activity was also not detected, with SI <10, ranging from 1.2 to 3.6. Conclusion: Although it is used in traditional medicine, Leaf and branch extracts from T. micrantha did not present antimicrobial activity, which could be caused by the antagonistic effect of the compounds present in the extract.Item Netzahualcoyonol from Salacia multiflora (Lam.) DC. (Celastraceae) roots as a bioactive compound against gram-positive pathogens.(2022) Nizer, Waleska Stephanie da Cruz; Ferraz, Ariane Coelho; Moraes, Thaís de Fátima Silva; Lima, William Gustavo de; Santos, Josana Pereira dos; Duarte, Lucienir Pains; Magalhães, Cíntia Lopes de Brito; Vieira Filho, Sidney Augusto; Magalhães, José Carlos deThe expression of virulence factors, such as biofilm formation, in association with the acquisition of resistance to multiple drugs, has evidenced the need for new and effective antimicrobial agents against Staphylococcus aureus. The evaluation of the pharmacological properties of plant-derived compounds is a promising alternative to the development of new antimicrobials. In this study, we aimed to evaluate the antibacterial, antibiofilm, and the synergistic and cytotoxic effects of netzahualcoyonol isolated from Salacia multiflora (Lam.) DC. roots. Netzahualcoyonol presented bacteriostatic (1.56–25.0 mg/mL) and bactericidal (25.0–400.0 mg/mL) effects against Gram-positive bacteria, disrupted the biofilm of S. aureus, and presented a synergistic effect after its combination with b-lactams and aminoglycosides. The low cytotoxicity of netzahualcoyonol (Selectivity Index (SI) for S. aureus (2.56), S. saprophyticus (20.56), and Bacillus subtilis (1.28)) suggests a good security profile. Taken together, these results show that netzahualcoyonol is promising for the development of a new effective antibacterial agent.Item Pristimerin isolated from Salacia crassifolia (Mart. Ex. Schult.) G. Don. (Celastraceae) roots as a potential antibacterial agent against Staphylococcus aureus.(2021) Nizer, Waleska Stephanie da Cruz; Ferraz, Ariane Coelho; Moraes, Thaís de Fátima Silva; Lima, William Gustavo de; Santos, Josana Pereira dos; Duarte, Lucienir Pains; Ferreira, Jaqueline Maria Siqueira; Magalhães, Cíntia Lopes de Brito; Vieira Filho, Sidney Augusto; Andrade, Ana Cláudia dos Santos Pereira; Rodrigues, Rodrigo Araújo Lima; Abrahão, Jônatas Santos; Magalhães, José Carlos deEthnopharmacological relevance: Pristimerin is a triterpenoid considered the main component of Salacia crassifolia extracts. This terpene has shown promising antitumor, anti-inflammatory, and antimicrobial effects. Likewise, S. crassifolia has been used in traditional medicine to treat cancer and as an antimicrobial and anti-inflammatory agent. Aim of the study: This study aimed to evaluate the antibacterial activity of the hexane extract of Salacia crassifolia roots (HER) and its isolate, pristimerin, against pathogenic bacteria. Materials and methods: First, we evaluated the spectrum of action of HER and pristimerin by the determination of the minimum inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC). Subsequently, we analyzed the time-kill curve of these plant-derived compounds against Staphylococcus aureus. Then, we examined their mode of action by three different assays: the crystal violet methodology, the release of intracellular material, and transmission electron microscopy methods (TEM). Finally, we evaluated the effect of HER and pristimerin on the pre-formed biofilm of S. aureus by the crystal violet assay, the synergistic effect by the checkerboard method, the cytotoxicity against Vero cells, and the in silico activity using the online software PASS. Results: HER and pristimerin presented a narrow spectrum of action against Gram-positive bacteria (MIC 0.195–25 μg/mL), and their primary mode of action is the alteration of membrane permeability of S. aureus. Our results show that the compounds disrupted the pre-formed biofilm of S. aureus in a dose-dependent manner. Furthermore, HER and pristimerin presented a significant synergic effect after the combination with well-known antibiotics, which was associated with the ability of these phytomedicines to change membrane permeability. Regarding the cytotoxic effect, the selective index (SI) of HER ranged from 0.37 to 11.86, and the SI of pristimerin varied from 0.24 to 30.87, according to the bacteria tested. Conclusions: Overall, HER and pristimerin showed a promising antibacterial effect in vitro through the alteration of membrane permeability of S. aureus.