Navegando por Autor "Leite, Jacqueline Isaura Alvarez"
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Item Consumption of conjugated linoleic acid (CLA)-supplemented diet during colitis development ameliorates gut inflammation without causing steatosis in mice.(2018) Moreira, Thais Garcias; Santos, Ana Cristina Gomes; Horta, Laila Sampaio; Miranda, Mariana Camila Gonçalves; Santiago, Andrezza Fernanda; Gonçalves, Juliana Lauar; Reis, Daniela Silva dos; Castro Junior, Archimedes Barbosa de; Santos, Luísa Lemos dos; Guimarães, Mauro Andrade de Freitas; Aguilar, Edenil Costa; Pap, Attila; Amaral, Joana Ferreira do; Leite, Jacqueline Isaura Alvarez; Machado, Denise Carmona Cara; Rezende, Rafael Machado; Nagy, Laszlo; Faria, Ana Maria Caetano de; Maioli, Tatiani UceliDietary supplementation with conjugated linoleic acid (CLA) has been proposed for weight management and to prevent gut inflammation. However, some animal studies suggest that supplementation with CLA leads to the development of nonalcoholic fatty liver disease. The aims of this study were to test the efficiency of CLA in preventing dextran sulfate sodium (DSS)-induced colitis, to analyze the effects of CLA in the liver function, and to access putative liver alterations upon CLA supplementation during colitis. So, C57BL/6 mice were supplemented for 3 weeks with either control diet (AIN-G) or 1% CLA-supplemented diet. CLA content in the diet and in the liver of mice fed CLA containing diet were accessed by gas chromatography. On the first day of the third week of dietary treatment, mice received ad libitum a 1.5%–2.5% DSS solution for 7 days. Disease activity index score was evaluated; colon and liver samples were stained by hematoxylin and eosin for histopathology analysis and lamina propria cells were extracted to access the profile of innate cell infiltrate. Metabolic alterations before and after colitis induction were accessed by an open calorimetric circuit. Serum glucose, cholesterol, triglycerides and alanine aminotransaminase were measured; the content of fat in liver and feces was also accessed. CLA prevented weight loss, histopathologic and macroscopic signs of colitis, and inflammatory infiltration. Mice fed CLA-supplemented without colitis induction diet developed steatosis, which was prevented in mice with colitis probably due to the higher lipid consumption as energy during gut inflammation. This result suggests that CLA is safe for use during gut inflammation but not at steady-state conditions.Item Efeito protetor do açaí (Euterpe oleracea Mart.) sobre a esteatose hepática, resistência à insulina e estresse oxidativo induzidos por dieta hiperlipídica em camundongos.(2015) Guerra, Joyce Ferreira da Costa; Pedrosa, Maria Lúcia; Silva, Marcelo Eustáquio; Pedrosa, Maria Lúcia; Martino, Hércia Stampini Duarte; Leite, Jacqueline Isaura Alvarez; Magalhães, Cíntia Lopes de BritoA crescente incidência da obesidade e comorbidades associadas está relacionada a um aumento paralelo da Doença Hepática Gordurosa não Alcoólica (NAFLD), uma das mais frequentes causas de doença crônica do fígado da atualidade. A NAFLD representa um espectro de condições caracterizadas por acúmulo de triacilgliceróis nos hepatócitos, que incluem a esteatose, esteato-hepatite, que pode evoluir para cirrose e carcinoma hepatocelular. Embora sua patogênese exata permaneça desconhecida, a hipótese mais aceita define que alterações metabólicas desencadeadas pela resistência à insulina levam ao desenvolvimento da esteatose hepática, considerada o primeiro evento, seguido do estresse oxidativo que contribui para a evolução do quadro. Compostos bioativos presentes em alimentos, principalmente polifenóis têm sido considerados promissores na prevenção de distúrbios metabólicos. O açaí (Euterpe oleracea Mart.) ganhou reconhecimento internacional devido ao seu alto conteúdo de polifenóis e capacidade antioxidante. Assim, o objetivo do presente estudo foi avaliar os efeitos do consumo de um extrato aquoso de açai (EAA) sobre alterações metabólicas desencadeadas pelo consumo de dieta hiperlipídica em camundongos. Camundongos Swiss machos foram divididos inicialmente em 2 grupos experimentais, um grupo (C) recebeu dieta padrão AIN-93M, e o outro grupo (HF) recebeu uma dieta hiperlipídica (32% de banha suína e 1% de colesterol) por seis semanas, após este período, os grupos C e HF foram subdivididos em quatro grupos de acordo com o tratamento recebido. Os grupos C e CA receberam dieta padrão, e os grupos HF e HFA receberam dieta hiperlipídica, os grupos CA e HFA receberam tratamento com o EAA (3g/Kg de peso corporal), administrado diariamente via gavagem por mais seis semanas. Camundongos do grupo HF apresentaram maior ganho de massa corporal associado à resistência à insulina, alterações no perfil das adipocinas TNFα e adiponectina, hepatomegalia, elevação dos níveis séricos das transaminases e esteatose hepática. Além disso, apresentaram alterações nos níveis de mRNA de genes relacionados ao metabolismo de lipídios hepático e aumento no estresse oxidativo. O tratamento com o EAA apresentou efeito protetor sobre a esteatose hepática, através da melhora nos níveis de adipocinas, sensibilidade a insulina e aumento na expressão dos genes relacionados a β-oxidação de ácidos graxos mediada por PPAR-α. E ainda, promoveu uma melhora no balanço oxidante/antioxidante hepático. Estes dados indicam que o açaí pode representar uma estratégia dietética viável, associada a prevenção e/ou tratamento de distúrbios metabólicos.Item Lecithin-based nanocapsule loading sucupira (Pterodon emarginatus) oil effects in experimental mucositis.(2022) Di Miceli, Jeruza Ferraz Filgueiras; Andrade, Maria Emília Rabelo; Carvalho, Paula Lopes Armond; Santos, Elandia Aparecida; Oliveira, Anna Eliza Maciel de Faria Mota; Fernandes, Caio Pinho; Cruz, Rodrigo Alves Soares; Garrett, Rafael; Mosqueira, Vanessa Carla Furtado; Cassali, Geovanni Dantas; D’Haese, Cecile; Nysten, Bernard; Leite, Jacqueline Isaura Alvarez; Cardoso, Valbert Nascimento; Araújo, Raquel SilvaIntestinal mucositis (IM) is a frequent adverse effect in anticancer therapy without standard treatment. The oil obtained from sucupira (Pterodon emarginatus) has anti-inflammatory properties, and the soybean lecithin re- duces the intestinal toxicity of several xenobiotics. However, their water insolubility impairs the in vivo appli- cation. For this reason, we evaluated if the nanoencapsulation of sucupira oil (SO) in lecithin-based nanocapsules (SO-NC) could be a therapeutically effective system for the treatment of IM in murine cisplatin (CDDP)-induced intestinal mucositis model. SO was analyzed by LC-HRMS/MS and HPLC. SO-NC was prepared by nano- precipitation and characterized using DLS, HPLC, and AFM. Mice body weight and food consumption were assessed daily during experimental mucositis induced by CDDP. The animals were euthanized, and intestinal permeability, inflammatory mediators, and intestinal histology were performed. SO-NC demonstrated adequate characteristics for oral administration as size under 300 nm, IP < 0.3, high EE, and spherical shape. In vitro cytotoxicity performed against RAW 264.7 cell lines resulted in cell viability above 80 % confirming the non- cytotoxic profile of SO (IC50 268 μg/mL) and SO-NC (IC50 118.5 μg/mL) up to 117.2 μg/mL. The untreated mice showed intestinal toxicity after i.p. of CDDP, principally weight loss, increased intestinal permeability, and MPO and TNF-α levels. Surprisingly, the administration of SO to CDDP-mucositis animals did not circumvent the CDDP effects and increased intestinal permeability. However, SO-NC proved efficient in mitigating the experi- mental intestinal mucositis by improving intestinal epithelium architecture, reducing intestinal permeability, and improving the MPO levels. In conclusion, SO-NC can positively impact intestinal mucositis by promoting mucosal recovery. This is a promising strategy for developing a new treatment for intestinal mucositis.Item Leishmania major self-limited infection increases blood cholesterol and promotes atherosclerosis development.(2013) Fernandes, Luciana Rodrigues; Ribeiro, Ana Cecília de Castro; Segatto, Marcela; Santos, Luís Felipe F. F.; Amaral, Joana Ferreira do; Portugal, Luciane Rodrigues; Leite, Jacqueline Isaura AlvarezLeishmania major infection of resistant mice causes a self-limited lesion characterized by macrophage activation and a Th1 proinflammatory response. Atherosclerosis is an inflammatory disease involving hypercholesterolemia and macrophage activation. In this study, we evaluated the influence of L. major infection on the development of atherosclerosis using atherosclerosissusceptible apolipoprotein E-deficient (apoE KO) mice. After 6 weeks of infection, apoE KO mice exhibited reduced footpad swelling and parasitemia similar to C57BL/6 controls, confirming that both strains are resistant to infection with L.major. L.majorinfected mice had increased plasma cholesterol levels and reduced triacylglycerols. With regard to atherosclerosis, noninfected mice developed only fatty streak lesions, while the infected mice presented with advanced lesions containing a necrotic core and an abundant inflammatory infiltrate. CD36 expression was increased in the aortic valve of the infected mice, indicating increased macrophage activation. In conclusion, L. major infection, although localized and self-limited in resistant apoE KO mice, has a detrimental effect on the blood lipid profile, increases the inflammatory cell migration to atherosclerotic lesions, and promotes atherogenesis.These effects are consequences of the stimulation of the immune system by L. major, which promotes the inflammatory components of atherosclerosis, which are primarily the parasite-activated macrophages.Item The polymorphism rs17782313 near MC4R gene is related with anthropometric changes in women submitted to bariatric surgery over 60 months.(2017) Resende, Cristina Maria Mendes; Durso, Danielle Fernandes; Borges, Karina Braga Gomes; Pereira, Rafaela Messias; Rodrigues, Gisele Kuhlmann Duarte; Rodrigues, Kathryna Fontana; Silva, José Luiz Padilha da; Rodrigues, Erica Castilho; Franco, Glória Regina; Leite, Jacqueline Isaura AlvarezObjective: Evaluate whether the polymorphism rs17782313 near MC4R gene influences long-term outcomes after bariatric surgery. Methods: The rs16782313 polymorphism was genotyped in 217 individuals undergoing bariatric surgery and analyzed in detail in 141 women. Data for comorbidities, BMI, excess weight loss (EWL), and body composition were obtained before and during 60 months after surgery. Results: The risk allele was found in 65 (47%) of the 141 women. Pre-surgical body weight and BMI were higher in carriers of the rs17782313 polymorphism (CC þ CT group) than in non-carriers (TT group) (p ¼ 0.039 and 0.047, respectively). The number of women who acquired surgical success (EWL > 50%), was lower in CC þ CT group compared to TT group (p ¼ 0.015). The minimum BMI seen during the 60 months of follow-up was higher in CC þ CT group compared to TT group (p ¼ 0.028). The number of women who presented BMI < 30 kg/m2 (no longer classified as obesity) after 24 months of surgery was inferior in CC þ CT group (6 out 35 patients e 17%) than in TT group (19 out 49 patients e 37%, p ¼ 0.043). Moreover, the number of patients maintaining BMI > 35 kg/m2 were higher carriers (18 out 35 patients e 51%) compare to non-carriers (16 out 49 patients e 32%, p ¼ 0.045). Conclusion: Women with extreme obesity carrying rs17782313 MC4R polymorphism present a higher pre-surgical BMI, are more unlikely to reach non-obesity BMI (<30 kg/m2) and tend to maintain a BMI > 35 kg/m2 that characterize treatment failure.