Navegando por Autor "Leite, Celisnolia Morais"
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Item Cytotoxic activity of Ru(II)/DPEPhos/N,S-mercapto complexes (DPEPhos = bis-[(2-diphenylphosphino)phenyl]ether).(2023) Grawe, Gregory Ferreira; Oliveira, Katia Mara de; Leite, Celisnolia Morais; Oliveira, Tamires Donizeth de; Costa, Analu Rocha; Moraes, Carlos André Ferreira; Araujo Neto, João Honorato de; Cominetti, Márcia Regina; Castellano, Eduardo Ernesto; Correa, Rodrigo de Souza; Machado, Sérgio de Paula; Batista, Alzir AzevedoWe report here on three new ruthenium(II) complexes, [Ru(DPEPhos)(mtz)(bipy)]PF6 (Ru1), [Ru(DPEPhos) (mmi)(bipy)]PF6 (Ru2) and [Ru(DPEPhos)(dmp)(bipy)]PF6 (Ru3). DPEPhos = bis-[(2-diphenylphosphino) phenyl]ether, mtz = 2-mercapto-2-thiazoline, mmi = 2-mercapto-1-methylimidazole, dmp = 4,6-diamino-2- mercaptopyrimidine and bipy = 2,2′ -bipyridine. The compounds were characterized by several spectroscopic techniques, and the molecular structure of Ru1 complex was determined by single-crystal X-ray diffraction. The cytotoxicity of Ru1 – Ru3 complexes were tested against the A549 (human lung) and the MDA-MB-231 (human breast) cancer cell lines and against MRC-5 (non-tumor lung) and MCF-10A (non-tumor breast) cell lines through the MTT assay. All three complexes are cytotoxic against the cell lines studied, with IC50 values lower than those found for the cisplatin. Among them, the Ru2 complex has shown the best selectivity against MDA-MB-231 cancer cell lines, with an IC50 value 12 times lower than that on MCF-10A. The complex Ru2 was capable to induce changes in MDA-MB-231 cells morphology, with loss of cellular adhesion, inhibited colony formation and induce an accumulation of cells at the sub-G1 phase, with an increase in S-phase and decrease of cells at G2 phase. Viscosity, electrochemical and Hoechst 33258 displacement experiments for Ru1 – Ru3 complexes with calf thymus DNA (CT-DNA) showed an electrostatic and groove binding mode of interaction. Additionally, the complexes interact with the protein Human Serum Albumin (HSA) by static mechanism. The negative values for ΔH and ΔS indicate that van der Waals forces and hydrogen bonding may occurs between the complexes and HSA. Therefore, this class of complexes are promising anticancer candidates and may be selected to further detailed studies.Item Dynamics of formation of binuclear metal complexes : a new Cu(I) compound with N-(2-thiophenecarbonyl)-N’-(3- Cl, 4-F-phenyl) thiourea as ligand.(2022) Ramos Cairo, Raúl; Plutín Stevens, Ana María; Mocelo Castell, Raúl Oscar; Castellano, Eduardo Ernesto; Correa, Rodrigo de Souza; Nossa González, Diana Lisseth; Erben, Mauricio Federico; Cominetti, Márcia Regina; Leite, Celisnolia Morais; Oliveira, Tamires Donizeth de; Batista, Alzir AzevedoHerein we report the synthesis and characterization of a new copper(I) complex with triphenylphosphine (PPh3) and N-(2- thiophenecarbonyl)-N’-(3-Cl, 4-F-phenyl)thiourea (HL), as ligands. The complex was characterized by vibrational (FTIR and FT-Raman) and multinuclear ( 1 H, 13C { 1 H}, 31P{1 H}) NMR spectroscopies. The crystalline structure of the complex was determined by single-crystal X-ray diffraction, confirming that a neutral binuclear compound, identified as [Cu(PPh3)(L-k2 -N,μ-S)]2, was obtained. The anionic thiourea ligand coordinates to the metal through the sulfur and nitrogen atoms in an unusual bidentate k2 -N,μ-S coordination mode. The complex is a dimer sited on a crystallographic center of symmetry. Each Cu2(μ-S)2 cation bridges trough the sulfur atoms of two symmetry related thiourea moieties and is also coordinated to the nitrogen atom of the thiourea ligand and the phosphorus atom from PPh3 coligand, forming a slightly distorted tetrahedral geometry. An intramolecular N H⋅⋅⋅O=C hydrogen bond is observed in the anionic ligand, forming a six-membered ring that stabilizes the N H thioamide group. Hirshfeld surface analysis shows that the molecules are connected by weak intermolecular contacts C⋅⋅⋅C, H⋅⋅⋅C and H⋅⋅⋅H which add to the stability of the crystalline packing. The in vitro cytotoxicity study of the complex indicates that it is more active against human lung carcinoma cells (A549), when compared to the free ligand.Item "Half-sandwich"/RuII anticancer complexes containing triphenylphosphine and p-substituted benzoic acids.(2020) Araujo Neto, João Honorato de; Oliveira, Katia Mara de; Leite, Celisnolia Morais; Colina Vegas, Legna Andreina; Nóbrega, Joaquim de Araújo; Castellano, Eduardo Ernesto; Ellena, Javier Alcides; Correa, Rodrigo de Souza; Batista, Alzir AzevedoMononuclear and binuclear RuII/arene/triphenylphosphine complexes with p-substituted benzoic acid derivatives were prepared and characterized. These monocationic complexes of type [Ru(η6 -p-cymene)(PPh3)L] (L = benzoic acid (1), p-hydroxybenzoic acid (2), p-nitrobenzoic acid (3) and terephthalic acid (4)) were characterized using various techniques, such as nuclear magnetic resonance (NMR) and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry, and the crystal structure of 1, 3 and 4 were determined by X-ray diffraction analysis. The cytotoxicity of the complexes was evaluated, in vitro, against tumorigenic [MDA-MB-231, MCF-7 (breast), A549 (lung) and DU-145 (prostate)] and non-tumorigenic [MCF-10A (breast), MRC-5 (lung) and PNT-2 (prostate)] cells. The binuclear complex (4) was inactive due to its low solubility. Complexes 1, 2 and 3 showed similar cytotoxicity, however, complex 1 presented better selectivity index against MDA-MB-231 than compounds 2 and 3. Cellular ruthenium absorption was explored by inductively coupled plasma mass spectrometry (ICP-MS) analyzing the whole cells and the culture medium. Complementary studies showed that complex 1 inhibited colony formation, induced morphology changes in cells and promoted cell cycle arrest in the Sub-G1 phase for the MDA-MB-231 cells.Item Nitrosyl/Diphenylphosphine/Amino Acid–Ruthenium complexes as inhibitors of MDA-MB-231 breast cancer cells.(2023) Barbosa, Marília Imaculada Frazão; Correa, Rodrigo de Souza; Macedo, Adriana Pereira Mundim Guedes; Graça, Alex Marchezini; Andrade, Francyelli Mello; Leite, Celisnolia Morais; Lacerda, Elisângela de Paula Silveira; Ellena, Javier Alcides; Silva, Henrique Vieira Reis; Doriguetto, Antônio Carlos; Batista, Alzir AzevedoHerein, we report on the synthesis and characterization of ruthenium compounds with the general formula [RuCl(AA-H)(NO)(dppb]PF6 , where AA = glycine (1), L-alanine (2), L-phenylalanine (3) and L-valine (4), and dppb = 1,4-bis(diphenylphosphine)butane. The complexes were characterized using elemental analysis, UV/Vis and infrared spectroscopies, 1H, 13C, 31P NMR techniques, and cyclic voltammetry. Furthermore, the structures of the compounds (1) and (3) were determined using single-crystal X-ray diffraction. In vitro evaluation of the Ru(II)/nitrosyl/amino acid complexes revealed their cytotoxic activities against triple-negative MDA-MB-231 breast cancer cells, and against the non-tumor murine fibroblast cells. All the compounds decreased the percentage of viable cells, inducing cell death by apoptosis. Additionally, the Ru(II) complexes inhibited the migration of MDA-MB-231 cells at concentrations lower than 35 µM, after 48 h of exposure. Thus, these complexes may be promising agents for the treatment of triple-negative MDA-MB-231 breast cancer.