Navegando por Autor "Kitajima, Elliot Watanabe"
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Item Morphological and molecular characterization of the Poxvirus BeAn 58058.(1998) Fonseca, Flávio Guimarães da; Lanna, Maria Célia da Silva; Campos, Marco Antônio da Silva; Kitajima, Elliot Watanabe; Péres, José Noronha; Golgher, Romain Rolland; Ferreira, Paulo César Peregrino; Kroon, Erna GeessienBeAn 58058 virus (BAV) was isolated from an Oryzomis rodent in Brazil. BAV was shown to be antigenically related to another poxvirus also isolated in Brazil, the Cotia virus, but it remained ungrouped. Electron microscopy revealed that BAV has a typical poxvirus morphology. The Hind III DNA profile of BAV genome was similar with that of VV WR and Lister, but some differences in the profile were detected. We have also detected the presence of genes homologous to vaccinia virus (VV WR) genes in the genome of BAV. Genes related to vaccinia thymidine kinase (TK) gene and vaccinia growth factor (VGF) gene were found. The patterns of TK and VGF mRNA transcripts described for vaccinia virus infected cells were observed in BAV infected cells. Nucleotide sequence of BAV VGF homologous gene was similar to VVWRVGF sequences. This similarity was further seen when cross-hybridization of total genomes of BAV and VV was done. Polypeptide synthesis of BAV and vaccinia in infected cells also showed similar profiles. The genetic data was used to construct a phylogenetic tree where BAV and VV were placed at the same cluster. Based on our findings we propose that BAV is a vaccinia virus variant.Item Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp. aurantifolii.(2010) Moreira, Leandro Marcio; Almeida Junior, Nalvo Franco de; Potnis, Neha; Digiampietri, Luciano Antonio; Adi, Said Sadique; Bortolossi, Julio Cesar; Silva, Ana Claudia Rasera da; Silva, Aline Maria da; Moraes, Fabrício Edgar de; Oliveira, Julio Cezar Franco de; Souza, Robson Francisco de; Facincani, Agda Paula; Ferraz, André Luiz Julien; Ferro, Maria Inês Tiraboschi; Furlan, Luiz Roberto; Gimenez, Daniele Fernanda Jovino; Jones, Jeffrey B.; Kitajima, Elliot Watanabe; Laia, Marcelo Luiz de; Leite Junior, Rui Pereira; Nishyama, Milton Yutaka; Rodrigues Neto, Julio; Dezem, Leticia Ane Sizuki Nociti; Norman, David J.; Ostroski, Eric Hainer; Pereira Junior, Haroldo Alves; Staskawicz, Brian J.; Tezza, Renata Izabel; Ferro, Jesus Aparecido; Vinatzer, Boris A.; Setubal, João CarlosBackground: Citrus canker is a disease that has severe economic impact on the citrus industry worldwide. There are three types of canker, called A, B, and C. The three types have different phenotypes and affect different citrus species. The causative agent for type A is Xanthomonas citri subsp. citri, whose genome sequence was made available in 2002. Xanthomonas fuscans subsp. aurantifolii strain B causes canker B and Xanthomonas fuscans subsp. aurantifolii strain C causes canker C. Results: We have sequenced the genomes of strains B and C to draft status. We have compared their genomic content to X. citri subsp. citri and to other Xanthomonas genomes, with special emphasis on type III secreted effector repertoires. In addition to pthA, already known to be present in all three citrus canker strains, two additional effector genes, xopE3 and xopAI, are also present in all three strains and are both located on the same putative genomic island. These two effector genes, along with one other effector-like gene in the same region, are thus good candidates for being pathogenicity factors on citrus. Numerous gene content differences also exist between the three cankers strains, which can be correlated with their different virulence and host range. Particular attention was placed on the analysis of genes involved in biofilm formation and quorum sensing, type IV secretion, flagellum synthesis and motility, lipopolysacharide synthesis, and on the gene xacPNP, which codes for a natriuretic protein. Conclusion: We have uncovered numerous commonalities and differences in gene content between the genomes of the pathogenic agents causing citrus canker A, B, and C and other Xanthomonas genomes. Molecular genetics can now be employed to determine the role of these genes in plant-microbe interactions. The gained knowledge will be instrumental for improving citrus canker control.