Navegando por Autor "Fontella, Fernanda Urruth"

Agora exibindo 1 - 2 de 2

Antidepressant-like effects of chronic guanosine in the olfactory bulbectomy mouse model.
(2021) Almeida, Roberto Farina de; Souza, Diogo Onofre; Nonose, Yasmine; Ganzella, Marcelo; Loureiro, Samanta Oliveira; Rocha, Andréia; Machado, Daniele Guilhermano; Bellaver, Bruna; Fontella, Fernanda Urruth; Leffa, Douglas Teixeira; Pettenuzzo, Letícia Ferreira; Venturin, Gianina Teribele; Greggio, Samuel; Costa, Jaderson Costa da; Zimmer, Eduardo Rigon; Elisabetsky, Elaine
Major depressive disorder (MDD) leads to pervasive changes in the health of afflicted patients. Despite advances in the understanding of MDD and its treatment, profound innovation is needed to develop fast-onset antidepressants with higher effectiveness. When acutely administered, the endogenous nucleoside guanosine (GUO) shows fast- onset antidepressant-like effects in several mouse models, including the olfactory bulbectomy (OBX) rodent model. OBX is advocated to possess translational value and be suitable to assess the time course of depressive-like behavior in rodents. This study aimed at investigating the long-term behavioral and neurochemical effects of GUO in a mouse model of depression induced by bilateral bulbectomy (OBX). Mice were submitted to OBX and, after 14 days of recovery, received daily (ip) administration of 7.5 mg/kg GUO or 40 mg/kg imipramine (IMI) for 45 days. GUO and IMI reversed the OBX-induced hyperlocomotion and recognition memory impairment, hippocampal BDNF increase, and redox imbalance (ROS, NO, and GSH levels). GUO also mitigated the OBX-induced hippocampal neuroinflammation (IL-1, IL-6, TNF-α, INF-γ, and IL-10). Brain microPET imaging ([18F]FDG) shows that GUO also prevented the OBX-induced increase in hippocampal FDG metabolism. These results provide additional evidence for GUO antidepressant-like effects, associated with beneficial neurochemical outcomes relevant to counteract depression.
Neurometabolic effects of sweetened solution intake during adolescence related to depressive-like phenotype in rats.
(2020) Arcego, Danusa Mar; Olivo, Laura Bem; Moraes, Rafael Oliveira; Garcia, Emily dos Santos; Silveira, Ana Caroline; Krolow, Rachel; Pereira, Natividade de Sa Couto; Lampert, Carine; Toniazzo, Ana Paula; Nicola, Fabrício do Couto; Sanches, Eduardo Farias; Aristimunha, Dirceu; Hoppe, Juliana Bender; Klein, Caroline Peres; Fontella, Fernanda Urruth; Almeida, Roberto Farina de; Gamaro, Giovana Duzzo; Froes, Fernanda Carolina Telles da Silva; Leite, Marina Concli; Netto, Carlos Alexandre; Zancan, Denise Maria; Dalmaz, Carla
Objective: Exposure to artificial sweeteners, such as aspartame, during childhood and adolescence has been increasing in recent years. However, the safe use of aspartame has been questioned owing to its potentially harmful effects on the developing brain. The aim of this study was to test whether the chronic consumption of aspartame during adolescence leads to a depressive-like phenotype and to investigate the possible mecha- nisms underlying these behavioral changes. Methods: Adolescent male and female rats were given unlimited access to either water, solutions of aspar- tame, or sucrose in their home cages from postnatal day 21 to 55. Results: Forced swim test revealed that both chronic aspartame and sucrose intake induced depressive-like behaviord, which was more pronounced in males. Additionally, repeated aspartame intake was associated with increased cerebrospinal fluid (CSF) aspartate levels, decreased hippocampal neurogenesis, and reduced activation of the hippocampal leptin signaling pathways in males. In females, we observed a main effect of aspartame: reducing PI3K/AKT one of the brain-derived neurotrophic factor pathways; aspar- tame also increased CSF aspartate levels and decreased the immunocontent of the GluN2A subunit of the N-methyl-D-aspartic acid receptor. Conclusion: The findings revealed that repeated aspartame intake during adolescence is associated with a depressive-like phenotype and changes in brain plasticity. Interestingly, males appear to be more vulnerable to the adverse neurometabolic effects of aspartame than females, demonstrating a sexually dimorphic