Navegando por Autor "Fonseca, Yasmim Arantes da"
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Item Biorrefinaria multiproduto do bagaço de malte : uma análise integrada das plataformas de extração de proteína seguida da produção de ácidos graxos voláteis por digestão anaeróbia.(2023) Fonseca, Yasmim Arantes da; Baeta, Bruno Eduardo Lobo; Gurgel, Leandro Vinícius Alves; Baeta, Bruno Eduardo Lobo; Silva, Silvana de Queiroz; Santos, André Bezerra dos; Gurgel, Leandro Vinícius Alves; Guimarães, Valeria Monteze; Dragone, Solange Ines Mussatto; Herrera Adarme, Oscar FernandoO bagaço de malte (BM) representa 85% do total de resíduos produzidos durante o processo de fabricação de cerveja, com um volume global de produção anual superior a 38 Mton. No Brasil, a maior parte desse resíduo agroindustrial é usado como suplementação nutricional para animais. Entretanto, no contexto da economia circular, essa biomassa pode ser fracionada para a obtenção de múltiplos produtos, dentre os quais as proteínas e os ácidos graxos voláteis (AGVs). Assim, nesse estudo foi avaliado o pré-tratamento alcalino sob pressão reduzida (PAPR) para extrair proteínas do bagaço de malte bruto, além da produção de ácidos carboxílicos por digestão anaeróbia a partir da fração sólida pré-tratada. A condição ótima do PAPR (T = 70oC, P = 455 mBar, t = 1 h e [NaOH] = 180 mmol L−1) foi capaz de remover 80% das proteínas do BM. Além disso, a condição otimizada foi seletiva (76,8%), teve uma pegada de carbono estimada reduzida (0,43 g CO2 g proteína extraída −1) e requer um tempo curto de reação (1 h). A remoção das proteínas do bagaço de malte não afetou o uso posterior da fração sólida para a produção de AGVs, como mostraram os resultados dos testes de digestão anaeróbia realizados com bagaço de malte in natura (BM-N) (cAGVs = 8005,3 mg L −1 ) e pré-tratado (BM-P) (cAGVs = 8126.6 mg L −1). O esterco bovino pré-tratado termicamente foi o melhor inóculo para a produção de AGVs a partir do BM, quando comparado aos tratamentos ácido, alcalino e com 2-bromoetanosulfato. A condição otimizada (T = 25oC e pH = 9) para a produção de AGVs a partir da fração sólida do bagaço de malte produziu 7900 mg L −1 de AGVs. Nos testes de fermentação, Clostridiaceae e Peptostreptococcaceae foram os membros mais abundantes nos reatores alimentados com BM-N. Por outro lado, nos reatores alimentados com BM-P a comunidade microbiana foi dominada pelas famílias Bacteroidaceae e Oscillospiraceae, que juntas representaram mais de 52% dos microrganismos identificados. A análise do ciclo de vida das tecnologias emergentes mostrou os pontos críticos para melhorar a atratividade das plataformas investigadas, quando comparada ao business as usual. A plataforma 2 (proteínas, gás natural e fertilizantes) foi a que apresentou menores emissões na categoria aquecimento global (2,4 kg CO2-eq). Para a plataforma multiproduto 3 (proteínas, AGVs, gás natural e fertilizantes) (10,0 kg CO2-eq) sugere-se que os estudos futuros concentrem os seus esforços em aumentar a eficiência de conversão da digestão anaeróbia visando a produção de AGVs.Item Enhancing biobased volatile fatty acids production from olive mill solid waste by optimization of pH and substrate to inoculum ratio.(2023) Fonseca, Yasmim Arantes da; Camargos, Adonai Bruneli de; Gomes, Gustavo Silva Magalhães; Lomar, Priscila Fregulia; Silva, Silvana de Queiroz; Gurgel, Leandro Vinícius Alves; Baeta, Bruno Eduardo LoboThe pH and substrate-to-inoculum ratio (S/I) are important parameters in the anaerobic fermentation of agroindustrial residues, and therefore the optimization of these two parameters is needed for a stable, efficient, and sustainable reactor operation. In this work, the parameters pH (5–9) and S/I (0.5–3 gVS gVS−1 ) were optimized to produce biobased volatile fatty acids (VFAs) from hydrothermally pretreated olive mill solid waste (HPOMSW). The response variables evaluated in the Doehlert design were total VFAs concentration (tVFAs) (mg L−1 ) and amounts (%) of isobutyric, butyric, isovaleric, and valeric acids on the VFAs profile. The pH was the variable that most influenced the mixed culture fermentation of HPOMSW, proving to be a key parameter in the process. Microbial community analyses of conditions 1 (S/I = 3 gVS gVS−1 and pH = 7) and 4 (S/I = 1.13 gVS gVS−1 and pH = 5) showed that Proteobacteria and Firmicutes accounted for more than 87% of the total microorganisms identified for both conditions. In addition, the second-order model best fitted the experimental data for the VFAs production at the desirable condition (S/I = 3 gVS gVS−1 and pH = 8).Item Influence of hydrothermal pretreatment conditions, typology of anaerobic digestion system, and microbial profile in the production of volatile fatty acids from olive mill solid waste.(2021) Fonseca, Yasmim Arantes da; Silva, Nayara Clarisse Soares; Camargos, Adonai Bruneli de; Silva, Silvana de Queiroz; Luna Wandurraga, Héctor Javier; Gurgel, Leandro Vinícius Alves; Baeta, Bruno Eduardo LoboThis study aimed to investigate the production of biobased volatile fatty acids (VFAs) from pretreated olive mill solid waste (OMSW). Three hydrothermal pretreatment (HP) conditions (D1: 125 oC, 53 min; D2: 161 oC, 62 min; D3: 191 oC, 83 min) were selected. The pretreated olive mill solid fraction (OMSF), pretreated olive mill liquid fraction (OMLF), and pretreated OMSW were evaluated as potential substrates for acidification in the anaerobic digestion (AD) in liquid (L-AD), semi-solid (Ss-AD), and solid (S-AD) phases. The best acidification efficiency (AE) observed in this study was 65%, for L-AD and HP condition D2 (161 oC, 62 min). However, the HP condition D2 applied to the Ss-AD provided a VFA concentration of 18218 mg L− 1 , about 6-fold higher than that observed in the L-AD system. Polyphenols changed the profile of VFAs, increasing the production of longer chain VFAs in the L-AD and S-AD systems. Also, the influence of the typology of the AD system and HP severity on the microbial community was evaluated. Proteobacteria and Firmicutes were the most representative phyla in acidogenic fermentation of OMSW substrates and the genera Enterobacter, Pseudomonas, Achromobacter, Clostridium, Ochrobactrum, and Peptoclostridium played an important role in the pretreated OMSW fermentation and, consequently, in the VFAs production profile.Item Methane production by anaerobic co-digestion of dairy manure and cassava wastes for energy recovery.(2022) Martins, Raissa Machado Maluf; Fonseca, Yasmim Arantes da; Faria, Marina Victal; Aquino, Sergio Francisco de; Herrera Adarme, Oscar Fernando; Baeta, Bruno Eduardo LoboBACKGROUND: In this study, the anaerobic co-digestion of cassava wastewater (CW), cassava bagasse (CB), and dairy manure (DM) was investigated to produce biogas with energy recovery. Mixture experimental design was performed to establish the proportion of each substrate, on the basis of total weight (%wt). The digestion was carried out under mesophilic conditions (35 °C) using a batch test. RESULTS: The best methane yields were 150.33 NmLCH4 gVS−1 (114.46 NmLCH4 gCOD−1 ) for the condition with 100% CW (C/N ratio = 14.04) and 130.43 normal mililiters of methane per gram of volatile solid (NmLCH4 gVS−1 ) and 93.84 normal mililiters of methane per gram of chemical oxygen demand (NmLCH4 gCOD−1 ) obtained with 66.7% DM, 16.7% CB and 16.7% CW (C/N ratio = 34.84). Experimental conditions containing high proportions of CB (above 33%) yielded high C/N ratios (above 52), resulting in low pH and high volatile fatty acid production, mainly acetic and butyric acids. Kinetics of methane production under optimal conditions were best described by Groot's multi-stage model. CONCLUSION: An energy balance for the best conditions were performed, leading to 452.86 MJ tonsubstrate−1 for a high DM content mixture experiment (DM = 66.7%, CB = 16.7%, CW = 16.7%); thereby showing that anaerobic co-digestion can replace 22.5% of the energy demanded by cassava processing industry. © 2022 Society of Chemical Industry (SCI).Item Produção de ácidos graxos voláteis (AGVs) biobaseados a partir da digestão anaeróbia do bagaço de oliva pré-tratado hidrotermicamente.(2020) Fonseca, Yasmim Arantes da; Baeta, Bruno Eduardo Lobo; Gurgel, Leandro Vinícius Alves; Baeta, Bruno Eduardo Lobo; Rodrigues, Fábio de Ávila; Aquino, Sergio Francisco deUm resíduo lignocelulósico produzido em grande quantidade no Brasil é o bagaço de oliva gerado durante a extração do azeite. Estima-se que para cada tonelada de azeitona processada sejam gerados 800 kg do resíduo. Com o intuito de inserir a indústria do azeite nos propósitos da economia circular biobaseada, o presente trabalho investigou a produção de ácidos graxos voláteis (AGVs) a partir da fermentação do bagaço de oliva pré-tratado hidrotermicamente. Nessa perspectiva, as condições experimentais do pré-tratamento hidrotérmico (PTH) do bagaço de oliva foram definidas por meio do planejamento experimental Doehlert, cujas variáveis independentes avaliadas foram temperatura (80 a 200 ºC) e tempo (30 a 100 min). Três condições de PTH (C1: 125ºC, 53min; C2: 161ºC, 62min; C3: 191ºC, 83min) foram selecionadas por meio da ferramenta desejabilidade do programa Statistica® para produzir substratos para a fermentação acidogênica em fase líquida (DA- L), semissólida (DA-Ss) e sólida (DA-S). Logo, a fração sólida (FS), a fração líquida (FL) e a mistura do bagaço bruto (B) pré-tratado foram avaliadas como substratos potenciais para acidificação durante a digestão anaeróbia (DA). O PTH melhorou a eficiência de acidificação (EA) em todos os sistemas de DA estudados, sendo que a C2 da DA-L apresentou 64,87% de EA. Em todos os cenários avaliados, o ácido acético foi o ácido predominante. Os compostos fenólicos, que apresentaram toxicidade a uma concentração maior que 6,34 g L - 1 para a DA-L, mudaram a rota metabólica dos microrganismos, aumentando a produção de ácidos graxos voláteis (AGVs) de cadeia longa nos sistemas em DA-L e DA-S. Além disso, o tipo de sistema de digestão anaeróbia e a severidade do PTH tiveram grande influência no perfil e na concentração dos AGVs produzidos, assim como na comunidade microbiana predominante. Os filos Proteobacteria e Firmicutes foram os mais representativos na DA acidogênica dos substratos oriundos do bagaço de oliva, com as espécies Enterobacter cloacae e Enterobacter hormaechei dominando a DA-L e DA- Ss. De qualquer forma, a C2 da DA-Ss gerou a combinação de concentração (19770 mg L-1 ) e perfil dos AGVs com maior rentabilidade econômica potencial(131$/ton). A otimização dos parâmetros do bioprocesso para essa condição foi realizada por uma matriz Doehlert que teve como variáveis independentes o pH (5-9) e a relação alimento/microrganismo (A/M) (0,5– 3 g SV g SV - 1 ). A função de desejabilidade foi aplicada e apontou que as maiores concentrações de AGVs com o maior percentual de ácidos de cadeia longa podem ser alcançados na condição pH=8 e relação A/M=3.Item Production of biogas and fermentable sugars from spent brewery grains : evaluation of one- and two-stage thermal pretreatment in an integrated biorefinery.(2021) Camargos, Adonai Bruneli de; Fonseca, Yasmim Arantes da; Silva, Nayara Clarisse Soares; Barreto, Elisa da Silva; Herrera Adarme, Oscar Fernando; Paranhos, Aline Gomes de Oliveira; Gurgel, Leandro Vinícius Alves; Baeta, Bruno Eduardo LoboIn this study, the production of fermentable sugars and biogas from thermally pretreated brewer’s spent grains (BSGs) was investigated. There were two autohydrolysis conditions (C1-1S: 180 oC, 30 min, 5 mLH2O gBSG − 1 and C2- 1S: 180 oC, 60 min, 5 mLH2O gBSG − 1 ) which were evaluated, with and without a previous low severity pretreatment (80 oC, 10 min, 10 mLH2O gBSG − 1 ). The highest specific methane production (302.4 NLCH4 kgCOD − 1 ) and enzymatic hydrolysis yield (EHY of 98%, 50 FPU gBSG − 1 ) were obtained from the two-stage pretreatment, with the second stage operated at 180 ◦C for 60 min, 5 mLH2O gBSG − 1 . However, in the integrated process, the two-stage pre- treatment with the second stage performed at 180 ◦C for 30 min, and 5 mLH2O gBSG − 1 was the best condition to produce fermentable sugars from enzymatic hydrolysis of pretreated BSGs, using a lower enzyme loading (EHY of 93%, 25 FPU gBSG − 1 ). The burning of biogas produced from an anaerobic digestion of liquid fractions (hydroly- sates) generated after two-stage thermal pretreatment in a combined heat and power system can produce a net thermal energy of 1.71 MJ kgBSG dry basis − 1 and electrical energy of 0.392 kW h kgBSG dry basis − 1 .Item Reduced-pressure alkaline pretreatment as an innovative and sustainable technology to extract protein from brewer’s spent grain.(2023) Fonseca, Yasmim Arantes da; Gurgel, Leandro Vinícius Alves; Baeta, Bruno Eduardo Lobo; Dragone, Giuliano; Dragone, Solange Ines MussattoOne of the bottlenecks related to the extraction of protein from lignocellulosic biomass is the development of an efficient, selective, economical, and environmentally friendly process. This study investigated the reducedpressure alkaline pretreatment (RPAP) as an innovative approach for protein extraction from brewer’s spent grain (BSG). Experiments were carried out at different temperatures (30–70 ◦C), pressures (150–1025 mBar), times (1–7 h), and NaOH concentrations (20–180 mM) to obtain a high-yield and selective protein extraction associated with low CO2 emissions and high processing capacity per batch. Protein concentration, total CO2 emissions per protein extracted, selectivity (related to sugars), and processing capacity were the responses considered for optimization. The optimum condition (T = 70 ◦C; P = 455 mBar, t = 1 h, and [NaOH] = 180.00 mM) was able to extract 80% of the total protein present in BSG composition. This condition was also selective (76.8%) and had a low carbon footprint (0.43 g CO2 g protein extracted − 1 ). Glutamic acid was the most abundant amino acid in both raw BSG (23.4%) and protein hydrolysate (24.7%), followed by leucine (9.5% and 8.4%, respectively), proline (8.4% and 11.8%, respectively), and aspartic acid (9.0% and 8.1%, respectively). RPAP was demonstrated to be an efficient and sustainable technology for protein extraction from BSG. This opens up new opportunities for BSG and other protein-rich lignocellulosic materials valorization in a biorefinery.Item Research progress, trends, and future prospects on hydrothermal liquefaction of algae for biocrude production : a bibliometric analysis.(2023) Bassoli, Sara Cangussú; Fonseca, Yasmim Arantes da; Luna Wandurraga, Héctor Javier; Baeta, Bruno Eduardo Lobo; Amaral, Mateus de SouzaThe rising demand to settle a sustainable energy source is guiding researchers in the production of biofuels. The liquefaction process is an alternative to obtaining biocrude from diferent types of renewable biomass and can mitigate environmental impacts. All papers published since 2000, which are related to the hydrothermal liquefaction process that aims to obtain biocrude are analyzed in the present study using the bibliometric approach to provide the selected database. Furthermore, the use of algae biomass in the liquefaction was also a discussed topic considering its high relevance in the process. The focus of the present study was to evaluate the evolution of the current state of the art in these topics and also to indicate trends and courses that it might be taken in the future. The database used in the bibliometric analysis was taken from the Web of Science (WoS) and the papers were selected by two diferent search equations. With the selected data, the use of BibExcel, VOSviewer, and PowerBi software was useful to guide the discussion and to create graphics and visual networks. As shown in the results, it was noticeable the infuence of China and the USA on the feld, considering the high number of publications from these countries. Moreover, the main authors were indicated considering their citation numbers, publications, and local h-index factor. Based on the author’s keywords, the most signifcant and recent topics on liquefaction were listed. Among them, technical-economic analysis, nutrient, and energy recovery, response surface methodology, and kinetic model are highlighted. This may indicate a new direction being taken by researchers besides the operational parameters’ studies.Item Tylosin in anaerobic reactors : degradation kinetics, efects on methane production and on the microbial community.(2022) Paranhos, Aline Gomes de Oliveira; Pereira, Andressa Rezende; Fonseca, Yasmim Arantes da; Silva, Silvana de Queiroz; Aquino, Sergio Francisco deTylosin eliminated in animal waste, during therapeutic treatment, can be efciently removed in anaerobic systems. The present study investigated the infuence of tylosin concentration and assessed its degradation kinetics and the microorganisms involved in each stage of its anaerobic digestion (hydrolysis/ acidogenesis; acetogenesis; methanogenesis). The results showed a stimulating efect on methane production with increasing tylosin concentration in the poultry litter up to 80 mg kg−1 tylosin (232.9 NL CH4 kg SV−1). As for tylosin degradation, greater removal of antibiotics was observed in the methanogenic phase (88%), followed by acetogenic (84%) and hydrolytic/acidogenic (76%) phases. The higher rate of tylosin degradation obtained in the methanogenic step, is mainly related to the co-metabolic efect exerted by the presence of acetate and its degradation by acetoclastic methanogens. Indeed, metagenomic analyses suggested a syntrophic action between archaea of the genus Methanobacterium, and bacteria such as Clostridium and Flexilinea, which seemed decisive for tylosin degradation.