Navegando por Autor "Flumignan, Danilo Luiz"
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Item Highly wet Chlorella minutissima biomass for in situ biodiesel production and residual biomass rich in labile carbohydrates.(2022) Pedro, Guilherme Arantes; Amaral, Mateus de Souza; Pereira, Félix Monteiro; Flumignan, Danilo Luiz; Rós, Patrícia Caroline Molgero da; Reis, Cristiano Eduardo Rodrigues; Silva, Messias BorgesThis study evaluated the use of biomass of Chlorella minutissima grown in 40-L photobioreactors using a Guillard f/2 biomass at over 80% moisture content as feedstock to produce ethyl esters. The study design investigated the infuence of acid catalyst type (HCl or H2SO4) and concentration (0.3 or 0.6 mol L−1), co-solvent to ethanol ratio (1:10 or 1:5 v/v), and acyl acceptor to biomass ratio (25 mL g−1 or 50 mL g−1) on ester yield and conversion. An L8 Taguchi design was used to evaluate the infuence of the factors, demonstrating a range of conversion ranging from 37 to 98%. Furthermore, the efects of reaction conditions on the residual biomass were assessed by measuring the concentration of acid-labile carbohydrates, which could be utilized in integrated sugar- and lipid-based algal refneries. It was found that in situ transesterifcation using HCl can provide easily accessible and fermentable carbohydrates at concentrations of about 32 wt% in residual biomass. In this sense, the fndings of this study demonstrate a further step in the integration of microalgae biomass in the development of liquid-fuel biorefneries.