Navegando por Autor "Dusse, Luci Maria Sant'Ana"
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Item Are the polymorphisms in ACE and ESR1 genes associated with preeclampsia occurrence?(2019) Lopes, Ana Cristina dos Santos; Perucci, Luiza Oliveira; Evangelista, Fernanda Cristina Gontijo; Godoi, Lara Carvalho; Sabino, Adriano de Paula; Silva, André Talvani Pedrosa da; Dusse, Luci Maria Sant'Ana; Alpoim, Patrícia NessrallaItem Association among ACE, ESR1 polymorphisms and preeclampsia in Brazilian pregnant women.(2019) Lopes, Ana Cristina dos Santos; Perucci, Luiza Oliveira; Evangelista, Fernanda Cristina Gontijo; Godoi, Lara Carvalho; Sabino, Adriano de Paula; Gomes, Karina Braga; Silva, André Talvani Pedrosa da; Dusse, Luci Maria Sant'Ana; Alpoim, Patrícia NessrallaBackground: Genetic, immune and environmental factors are involved in preeclampsia (PE) etiopathogenesis. Considering that hypertension and poor placental perfusion are important features in PE, polymorphisms in the angiotensin-converting enzyme (ACE) and estrogen nuclear receptor 1 (ESR1) genes could be involved in the predisposition and/or development of the disease. The aim of this study was to evaluate if polymorphisms in ACE and ESR1 genes were associated with PE occurrence. Material and Methods: This case-control study included 209 Brazilian pregnant women (107 with severe PE and 102 normotensive controls). The polymorphisms were investigated by polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis. Results: No significant difference between PE versus normotensive pregnant women, as well as early versus late PE, was observed when compared the allelic and genotypic frequencies of insertion/deletion polymorphism in intron 16 of the ACE gene and the single nucleotide polymorphisms (SNPs - rs2234693 and rs9340799) of the ESR1 gene. Conclusion: This pioneer study involving Brazilian women showed no association among the studied polymorphisms and PE, which suggests that ins/del ACE and SNPs ESR1 do not contribute to this disease occurrence in Brazil.Item Longitudinal assessment of D-dimer and plasminogen activator inhibitor type-1 plasma levels in pregnant women with risk factors for preeclampsia.(2019) Lucena, Flávia Campos; Lage, Eura Martins; Teixeira, Patrícia Gonçalves; Barbosa, Alexandre Simões; Diniz, Rejane Silva; Lwaleed, Bashir; Silva, André Talvani Pedrosa da; Alpoim, Patrícia Nessralla; Perucci, Luiza Oliveira; Dusse, Luci Maria Sant'AnaObjective: Investigating D-Dimer/D-Di and plasminogen activator inhibitor type-1/PAI-1 levels throughout gestation in women with preeclampsia/PE risk factors. Methods: D-Di and PAI-1 plasma levels were determined in 28 women at 12–19, 20–29, 30–34 and 35–40 weeks of gestation. Results: D-Di was lower at 12–19 weeks and higher at 30–34 weeks in women who developed PE versus who did not develop it. D-Di increased throughout gestation in both groups, peaking earlier in pregnant women who developed PE versus who did not develop it. PA1-1 increased across gestation, but it didn’t differ between groups. Conclusion: D-Di was able to discriminate these groups of women at 12–19 and 30–34 weeks of gestation.Item Neuroserpin : a potential biomarker for early-onset severe preeclampsia.(2023) Perucci, Luiza Oliveira; Silva, Sirlaine Pio Gomes da; Bearzoti, Eduardo; Pinto, Kelerson Mauro de Castro; Alpoim, Patrícia Nessralla; Pinheiro, Melina Barros; Godoi, Lara Carvalho; Moraes, Lauro Ângelo Gonçalves de; Sousa, Lirlândia Pires de; Dusse, Luci Maria Sant'Ana; Silva, André Talvani Pedrosa daPreeclampsia is a hypertensive disease of pregnancy associated with intense inflammatory and pro-coagulant responses. Neuroserpin is a serine protease inhibitor that has been involved in neurological and immune pro- cesses and has not yet been investigated in preeclampsia. Herein, we evaluated neuroserpin levels in association with other inflammatory mediators (IL-17A, IL-33, and CXCL-16) during severe preeclampsia. The mediators’ plasma levels were measured by immunoassays in 24 pregnant women with severe preeclampsia (early pre- eclampsia: N = 17, late preeclampsia: N = 7), 34 normotensive pregnant women, and 32 non-pregnant women. In general, pregnancy was associated with higher levels of neuroserpin, IL-17A, IL-33, and CXCL-16 than the non- pregnant state. However, this increase was attenuated in pregnancies complicated by severe preeclampsia. Although neuroserpin levels did not differ between normotensive pregnant women and pregnant women with severe preeclampsia, neuroserpin levels tended to be lower in early-onset than in late-onset severe preeclampsia. There were positive correlations between neuroserpin and IL-17A, neuroserpin and CXCL-16, and IL-17A and CXCL-16 levels in women with severe preeclampsia. In addition, although the risk for developing severe pre- eclampsia was higher in older women in this study, maternal age did not significantly influence the mediators’ levels, nor their correlations in the preeclampsia group. In summary, our data suggest that neuroserpin might be a potential biomarker for early-onset severe preeclampsia and, that the imbalance among neuroserpin, IL-17A, IL-33, and CXCL-16 levels may be associated with the pathogenesis of preeclampsia, regardless of the maternal age.Item A new index to discriminate between iron deficiency anemia and thalassemia trait.(2016) Matos, Januária Fonseca; Dusse, Luci Maria Sant'Ana; Borges, Karina Braga Gomes; Castro, Ricardo L. V. de; Vital, Wendel Coura; Carvalho, Maria das GraçasBackground: The most common microcytic and hypochromic anemias are iron deficiencyanemia and thalassemia trait. Several indices to discriminate iron deficiency anemia fromthalassemia trait have been proposed as simple diagnostic tools. However, some of the bestdiscriminative indices use parameters in the formulas that are only measured in moderncounters and are not always available in small laboratories.The development of an index with good diagnostic accuracy based only on parametersderived from the blood cell count obtained using simple counters would be useful in theclinical routine. Thus, the aim of this study was to develop and validate a discriminativeindex to differentiate iron deficiency anemia from thalassemia trait. Methods: To develop and to validate the new formula, blood count data from 106 (thalassemiatrait: 23 and iron deficiency: 83) and 185 patients (thalassemia trait: 30 and iron deficiency:155) were used, respectively. Iron deficiency, _-thalassemia trait and _-thalassemia trait wereconfirmed by gold standard tests (low serum ferritin for iron deficiency anemia, HbA2> 3.5%for _-thalassemia trait and using molecular biology for the _-thalassemia trait).Results: The sensitivity, specificity, efficiency, Youden’s Index, area under receiver operatingcharacteristic curve and Kappa coefficient of the new formula, called the Matos & CarvalhoIndex were 99.3%, 76.7%, 95.7%, 76.0, 0.95 and 0.83, respectively. Conclusion: The performance of this index was excellent with the advantage of being solelydependent on the mean corpuscular hemoglobin concentration and red blood cell countobtained from simple automatic counters and thus may be of great value in underdevelopedand developing countries.Item Pre-eclampsia is associated with reduced resolvin D1 and maresin 1 to leukotriene B4 ratios in the plasma.(2019) Perucci, Luiza Oliveira; Santos, Talita Adriana Pereira dos; Santos, Patrícia Campi; Teixeira, Lívia Cristina Ribeiro; Alpoim, Patrícia Nessralla; Gomes, Karina Braga; Sousa, Lirlândia Pires; Dusse, Luci Maria Sant'Ana; Silva, André Talvani Pedrosa daProblem: Omega-3 and omega-6 fatty acids can be endogenously converted into mediators with pro-inflammatory (eg, leukotriene B4/LTB4) or anti-inflammatory/ pro-resolving activities (eg, resolvin D1/RvD1 and maresin 1/MaR1). Recent data indicate an imbalance of LTB4 and MaR1 levels in pre-eclampsia (PE), but the relative production of these mediators, including RvD1, and the role of these mediators in the disease pathogenesis remain unclear. Therefore, this study aimed to investigate the plasma levels of LTB4, RvD1, and MaR1 in pregnant women with or without PE and non-pregnant controls and their association with clinical/laboratory parameters of PE women. Method of study: LTB4, RvD1, and MaR1 plasma levels were measured by competitive enzyme immunoassay in 19 non-pregnant, 20 normotensive pregnant, and 21 PE women. Results: Plasma concentrations of LTB4 were higher and RvD1 were lower in PE women than in normotensive pregnant women, who presented higher levels of LTB4 and similar levels of RvD1 to non-pregnant women. MaR1 levels did not differ among the groups. Pre-eclampsia women had decreased RvD1/LTB4 and MaR1/LTB4 ratios. Considering only the PE group, positive correlations were observed among all the mediators tested, between LTB4 and white blood cell count and between RvD1 and creatinine levels. However, all lipid mediators correlated negatively with body mass index before pregnancy. LTB4 also correlated negatively with maternal age. Conclusion: Our findings suggest that the PE state results in systemic overproduction of LTB4 in relation to RvD1 and MaR1, and that these lipid mediators may be involved with the disease pathogenesis.Item Response to the assessment of the Matos & Carvalho index by Hoffmann and Urrechaga.(2017) Matos, Januária Fonseca; Dusse, Luci Maria Sant'Ana; Borges, Karina Braga Gomes; Vital, Wendel Coura; Carvalho, Maria das Graças