Navegando por Autor "Dun, Nae J."
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Item Differential expression of cocaine- and amphetamine-regulated transcript-immunoreactivity in the rat spinal preganglionic nuclei.(2000) Dun, S. L.; Chianca Júnior, Deoclécio Alves; Dun, Nae J.; Yang, J.; Chang, Jaw-KangThe distribution of cocaine- and amphetamine-regulated transcript-like immunoreactivity (CART-LI) was investigated in the rat spinal cords with the use of an antiserum against the CART peptide fragment 55±102. CART-LI ®bers were concentrated in the super®cial layers of the dorsal horn of all segments. In addition to CART-LI ®bers, intensely labeled somata were detected in the intermediolateral cell column (IML) and other sympathetic preganglionic nuclei of the thoracolumbar segments. In the lumbosacral segments, CART-LI ®bers but not somata were seen in the sacral parasympathetic nucleus. Double-labeling the spinal sections with choline acetyltransferase (ChAT)-antisera and CARTantisera revealed that the large majority of ChAT-positive somata in the sympathetic preganglionic nuclei were CARTpositive, whereas ChAT-positive somata in the parasympathetic preganglionic nuclei were CART-negative. Our results show that CART-LI is selectively expressed in a population of sympathetic preganglionic neurons (SPNs), but not in parasympathetic preganglionic neurons (PPNs) of the rat.Item A low protein diet causes an increase in the basal levels and variabiltiy of mean arterial pressure and heart rate in fisher rats.(2013) Oliveira, Eduardo Luiz de; Cardoso, Leonardo Máximo; Pedrosa, Maria Lúcia; Silva, Marcelo Eustáquio; Dun, Nae J.; Colombari, Eduardo; Moraes, Márcio Flávio Dutra; Chianca Júnior, Deoclécio AlvesThe correlation between nutrition and cardiovascular related disorders is a well-established fact. Previous work from our Laboratory has suggested a significant compromise of cardiovascular reflexes in conscious rats submitted to a low-protein (LP) diet. Our working hypothesis is that the basal level of mean arterial pressure (MAP), variability of the mean arterial pressure (VMAP), heart rate (HR) and variability of heart rate (VHR) are altered in rats submitted to a protein restricted diet. Two experimental groups were used: control group (normal protein 15%, NP) and malnourished group (lowprotein 6%, LP). In order to verify the efficiency of the dietary restriction we measured body weight, total blood protein, plasma albumin, urea and glucose. Our experiments demonstrated that the malnourished rats presented augment levels of basal MAP (LP 122 6 2mmHg vs. NP 113 6 1mmHgÞ and of VMAP (LP 12:8 6 1:5mmHg vs. NP 9 6 1mmHgÞ when compared to the control group. We observed similar increased levels, in the malnourished group, for both HR (LP 429 6 8bpm vs. NP 381 6 7bpmÞ and VHR (LP 67:6 6 8:3bpm vs. NP 44:4 6 4:9bpmÞ: Our results suggest a correlation between the LP diet in Fisher rats and the increased basal levels of mean arterial pressure, HR and their respective variability.