Navegando por Autor "Cunha, Warlley Rosa"

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Angiotensin‐converting enzyme gene (ACE) polymorphisms are associated with dysregulation of biochemical parameters in hypertensive patients.
(2023) Agostini, Lívia da Cunha; Cunha, Warlley Rosa; Silva, Nayara Nascimento Toledo; Melo, André Sacramento; Soares, Luciana Bicalho Moreira; Almeida, Tamires Cunha; Belo, Vanessa de Almeida; Vital, Wendel Coura; Teixeira, Luiz Fernando de Medeiros; Lima, Angélica Alves; Silva, Glenda Nicioli da
Introduction The genetic component, including genes and their variants, plays a signifcant role in the pathophysiology of arterial hypertension (AH). Thus, clinical, epidemiological and genetic studies have been carried out to improve the under- standing of disease mechanisms, improve diagnostic quality and contribute to prevention. Objective To determine the association of risk factors, biochemical parameters and diferent ACE gene polymorphisms with AH. Method The case-control study was carried out in the population of Ouro Preto, Brazil. The subjects answered a question- naire containing clinical and sociodemographic data. The ACE gene polymorphisms rs4291, rs4363 and rs4335 were evalu- ated by real time-polymerase chain reaction (real-time PCR) in 310 people (155 hypertensive and 155 normotensive patients), in addition to biochemical parameters. A multivariate logistic regression model was used to identify factors associated with AH. Analysis of continuous variables was performed using the Kruskal-Wallis test to assess signifcance between groups and Dunn’s post-test for multiple comparisons. Results The results showed that AH was associated with age, education, smoking, obesity and high levels of triglycerides, sodium, glucose and uric acid. Regarding the biochemical parameters, in hypertensive patients, the rs4363 and rs4335 poly- morphisms were associated with high levels of triglycerides, urea and glucose; the rs4291 polymorphism was associated with elevated urea and glucose levels. No association was detected between SNPs and HA. Conclusion AH was associated with socioeconomic status, lifestyle habits and biochemical parameters. ACE polymorphisms may have infuenced the levels of triglycerides, urea and glucose in hypertensive patients.
Efeitos de polimorfismos nos genes ADIPOQ e NOS3 e fatores de risco no controle e nos níveis de pressão arterial em hipertensos.
(2022) Cunha, Warlley Rosa; Belo, Vanessa de Almeida; Lima, Angélica Alves; Paula, Gustavo Henrique Oliveira de; Belo, Vanessa de Almeida; Soares, Fernanda Rodrigues; Nascimento, Renata Cristina Rezende Macedo do
O tratamento e o controle da hipertensão arterial (HA) são essenciais para reduzir a morbimortalidade das doenças cardiovasculares. Enquanto polimorfismos de nucleotídeo único (SNPs) nos genes adiponectina (ADIPOQ) e óxido nítrico sintase endotelial (NOS3) têm sido associados a distúrbios cardiovasculares, a contribuição desses SNPs para o controle e níveis de pressão arterial (PA) em hipertensos com adesão à terapia ainda não está claro. Particularmente, não há estudos testando os efeitos dos polimorfismos da ADIPOQ no controle da pressão arterial. Neste sentido, o objetivo deste estudo foi avaliar se os genótipos, haplótipos e a interação entre os SNPs dos genes NOS3 (rs3918226 e rs1799983) e ADIPOQ (rs266729 e rs1501299) afetam o controle e os níveis de PA em hipertensos em tratamento com anti- hipertensivos. Foram recrutados, em um estudo transversal de fevereiro a setembro de 2021, 196 hipertensos adeptos a terapia onde a média das três últimas medidas de PA obtida dos prontuários foi usada para agrupá-los em: HA controlada (PA <140 / 90mmHg) e HA não controlada (PA ≥ 140 / 90mmHg). Amostras de sangue venoso foram coletadas destes indivíduos e os genótipos foram determinados pelo ensaio de discriminação de alelos Taqman®. As frequências dos haplótipos foram estimadas usando o programa haplo.stats. Análises de regressão logística múltipla e regressão linear múltipla foram realizadas ajustando para etnia, dislipidemia, obesidade, doenças cardiovasculares e níveis de ácido úrico. Todos os SNPs apresentaram equilíbrio de Hardy-Weinberg. O genótipo CG para o polimorfismo rs266729 do gene ADIPOQ apresentou associação com a HA não controlada e com maiores níveis de pressão arterial sistólica (PAS) e média (PAM) (P<0,05). Além disso, o haplótipo GT dos polimorfismos rs266729 e rs1501299 do ADIPOQ foi associado a HA não controlada e a maiores níveis de pressão arterial diastólica (PAD) e PAM em comparação com o haplótipo CT. Não foram observados efeitos dos genótipos ou haplótipos dos SNPs do gene NOS3 nem a interação com SNPs do gene ADIPOQ no controle da PA em hipertensos em tratamento. Os resultados deste trabalho sugerem que polimorfismos no gene ADIPOQ influenciam o controle da HA e níveis pressóricos de hipertensos em tratamento, surgindo como potencial marcador para resposta a terapia anti-hipertensiva.
High molecular weight adiponectin as a biomarker of hypertension in children and adolescents with obesity.
(2023) Cunha, Warlley Rosa; Gaspar, Isabella Campos; Souza, Beatriz Cazarim de; Martins, Bárbara Dias Lana; Miranda, Josiane Aparecida de; Lanna, Carla Márcia Moreira; Santos, José Eduardo Tanus dos; Lacchini, Riccardo; Belo, Vanessa de Almeida
Lower HMW (high molecular weight) adiponectin levels are associated with obesity, insulin resistance, and metabolic syndrome in children and adolescents. However, data on HMW levels in pediatric population with hypertension are lacking. This study aimed to examine the association and predictive capacity of HMW levels, HMW/HOMA-IR, and HMW/APN ratio with hypertension in obese children and adolescents. The 299 pediatric subjects were grouped in obese hypertensive (OH), obese normotensive (ON), and normal weight normotensive (NN). Plasma concentrations of HMW were investigated by ELISA. ANOVA was used to compare study groups, and a binary logistic regression analysis was used to verify if HMW, HMW/HOMA-IR, HMW/APN, APN, APN/HOMA-IR, and HOMA-IR are associated to hypertension regardless obesity in children and adolescents. To compare the strength and performance of each biomarker to classify individuals with and without hypertension, the receiver-operating characteristic (ROC) curve, area under the curve (AUC), and Youden index (J) were evaluated. Both HMW plasma levels and the HMW/HOMA-IR ratio were significantly lower in the OH group when compared to the ON group (HMW: 2.00±1.33 μg/mL vs 2.48±1.48 μg/mL; HMW/HOMA-IR ratio: 0.87±0.95 vs 1.27±1.2; P<0.05) and NN weight groups (HMW: 2.00±1.33 μg/mL vs 4.02±1.99 μg/mL; HMW/HOMA-IR ratio: 0.87±0.95 vs 2.62±1.86; P<0.05). Hypertension was associated with lowest HMW (OR=4.50; 95% CI=1.41–15.84) and HMW/HOMA-IR (OR=12.13; 95% CI=2.51–92.93) regardless of obesity. However, HOMA-IR or the HMW/APN was not significant (P>0.05). In the ROC curve analyses, the HMW and HMW/HOM-IR were more sensitive to detect hypertension in children and adolescents with obesity. Conclusion: Low levels of HMW oligomer and HMW/HOM-IR are associated with hypertension in childhood obesity. Thus, these biomarkers could be clinically useful in identifying hypertension in childhood obesity.