Navegando por Autor "Chaves, Jaísa Oliveira"

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Compliance in handling of donated raw breast milk to human milk banks regarding microbiological quality.
(2022) Chaves, Jaísa Oliveira; Fernandes, Angelica Maria de Freitas; Parreiras, Paola Machado; Passos, Maria Cristina; Cunha, Luciana Rodrigues da; Salierno, Camila Carvalho Menezes
Objectives: microbiological quality of raw human milk (HM) from donors at the HMB (Human Milk Bank) of Santa Casa de Misericórdia de Ouro Preto was evaluated. Methods: the microbiological quality of milk from each of 10 donors in triplicate (30 samples) was evaluated by enumeration of Staphylococcus aureus and total aerobic mesophiles (surface plating) and total and thermotolerant coliforms (Most Probable Number - MPN). A questionnaire and a checklist were applied after the end of sample collection to verify the compliance of milk extraction and storage procedures by the donors. Results: inadequacies in the procedures for expression and storage of donated HM were observed. The presence of all microorganisms studied were verified in the milk of all donors, and in some cases exceeded the safety limits. The checklist indicated that some necessary hygienic-sanitary procedures were not carefully performed, making it important to properly guide the donors. Conclusions: this study points to an unfavorable scenario for the safety and quality of donated HM, and it is important to encourage further studies to be conducted so that the Brazilian network of HMB adopts stricter criteria for instructions on procedures and support for HM donors.
Effect of storage on retinol content and total antioxidant capacity of human milk.
(2019) Chaves, Jaísa Oliveira; Fernandes, Angelica Maria de Freitas; Parreiras, Paola Machado; Breguez, Gustavo Silveira; Passos, Maria Cristina; Cunha, Luciana Rodrigues da; Salierno, Camila Carvalho Menezes
Purpose – The purpose of this paper is to evaluate the effect of different times and freezing temperatures on the antioxidant activity of raw human milk (HM) and the impact of light by different packaging on retinol level and the antioxidant activity of pasteurized HM. Design/methodology/approach – Donor milks were homogenized to form the pool of the experimental study characterized by the evaluation of the effects of time (0, 2, 4, 8 and 15 days) freezing temperatures (−3°C, −8°C and −18°C) and the interference of the type of packaging on the antioxidant activity and retinol levels of HM. Findings – The existing studies do not reveal the real impact of HM storage conditions adopted by human milk banks (HMB) in Brazil on their compounds, mainly in relation to the effects of temperature and freezing time and the incidence of light on retinol levels and antioxidant activity. In view of the already documented importance of these compounds for the growth, development and health of children, it is extremely important to assess their stability according to the procedures adopted by the banks. It has been observed in this study that lower freezing temperatures (−18°C) further preserve the antioxidant activity. It was found that the amber and transparent vials wrapped with aluminum foil allowed for greater retinol stability of HM, with values of 2.501±0.757 µmol/L and 4.991±0.825 µmol/L, respectively. On the contrary, there was no significant influence on antioxidant activity. Originality/value – It is suggested that HMB store milk at lower temperatures and use glass jars that block the passage of light.
Hygienic-sanitary quality and effect of freezing time and temperature on total antioxidant capacity of human milk.
(2021) Lopes, Lilian Maria Peixoto; Chaves, Jaísa Oliveira; Cunha, Luciana Rodrigues da; Passos, Maria Cristina; Menezes, Camila Carvalho
Knowledge of the hygienic-sanitary quality of raw human milk helps to establish the best storage conditions for the preservation of its nutritional and functional components. Therefore, this study aimed to evaluate the hygienic-sanitary quality of human milk extracted at the mothers’ homes, and to analyze the effect of freezing time and temperature on the milk’s total antioxidant capacity. Milk of five mothers was evaluated individually for Staphylococcus aureus, total aerobic mesophilic counts, mold and yeasts, total and thermotolerant coliforms. The milk pool was stored at -8 °C, -18 °C and -40 °C for 2, 4, 8 and 15 days and analyzed for the total antioxidant capacity by the 2,2-diphenyl-1-picrylhydrazyl free radical and 2,2-azino 3-ethylbenzothiazoline-6-sulfonic acid scavenging methods. The microbiological analyses of the raw human milk samples indicated counts of all the studied microorganisms above the safe limits. Related to the effect of freezing time and temperature on total antioxidant capacity, it was concluded that, regardless of the freezing temperature, a significant reduction occurred on total antioxidant capacity over time and that the lower the storage temperature, the greater its total antioxidant activity.
Interference of germination on the nutritional composition and antioxidant capacity of black sesame (Sesamum indicum L.).
(2021) Basso, Ana Carolina Dias; Machado, Pollyana Mara Ribeiro; Chaves, Jaísa Oliveira; Parreiras, Paola Machado; Salierno, Camila Carvalho Menezes
Purpose – The purpose of this study was to evaluate the effect of black sesame germination on its nutritional composition and antioxidant activity. Design/methodology/approach – Germination occurred at constant temperature and humidity (35 8C and 95 ± 5%) for 72 h. The levels of moisture, protein, lipids, ash, total dietary fibre and fractions, carbohydrates, calories, phenolic compounds and antioxidant capacity were determined. Findings – With germination, there was a significant increase in moisture and protein content, as well as a reduction in the other parameters assessed in terms of nutritional composition. When analysing bioactive compounds, no significant change was found in the content of phenolic compounds; however, there was a reduction in antioxidant capacity according to the three methods tested, which may be related to the characteristics of the grain itself, the conditions used for germination, or even the process of extracting phenolic compounds for analysis. Other conditions for the germination of black sesame using different times and temperatures should be evaluated in future studies. Originality/value – This is an original research article, which has an industrial and health impact by transmitting highly relevant responses on nutrition and food. The novelty of this research is the fact of studying the germination of black sesame on the nutritional composition (moisture, protein, lipids, ash, total dietary fibre and fractions, carbohydrates and calories), levels of phenolic compounds and antioxidant capacity. These results can help improve the procedures adopted by the food industries and also in nutritional conduct, as the germination process leads to a change in the nutritional composition, especially with regard to protein synthesis, which is relevant, once it is an interesting protein option. However, it also brings us responses to reductions in important nutritional compounds.
Maceration time over antioxidant activity, volatile compounds and temporal dominant sensation of fermented jabuticaba.
(2022) Paula, Daiane Gabriele Couto de; Vasconcelos, Christiane Mileib; Pereira, Alexandre Fontes; Quintão, Anna Lídya da Cunha; Chaves, Jaísa Oliveira; Parreiras, Paola Machado; Salierno, Camila Carvalho Menezes
Jabuticaba is a fruit sensory appreciated and has significant nutritional and functional value. However, it is quite perishable, and its use is interesting for the preparation of other products such as fermented jabuticaba, from a nutritional, biological and technological point of view. This study evaluated the interference of maceration time over antioxidant capacity, volatile compounds profile and temporal dominant sensation of fermented jabuticaba. Four different treatments of fermented jabuticaba in different fermentation times were prepared (96, 120, 144 and 168 h). After preparation, total monomeric anthocyanin content, total antioxidant activity by 2,2-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid and iron reduction method, volatile compounds profile by gas chromatography and sensory analysis of temporal dominance of sensations were performed of fermented jabuticaba. The highest concentration of total anthocyanins (25.81 mg/100 g) and antioxidant activity (78.34 μmol Trolox/g e 84.69 μM FeSO4 /g) of jabuticaba is in its skin. Regarding the antioxidant activity by FRAP method, the fermented beverages presented higher value than the whole fruit. However, after 96 h of maceration, the values decreased, followed by an increase after 144 h. In all maceration times the predominant sensation was acidic taste, initially and then astringency, the most persistent sensation in all treatments. However, there was a downward trend in this sensation with the increase in maceration time, as well as isoamyl alcohol content.
Qualidade microbiológica e efeito das condições de congelamento e da incidência de luz sobre os teores de retinol e capacidade antioxidante total do leite humano.
(2018) Chaves, Jaísa Oliveira; Menezes, Camila Carvalho; Cunha, Luciana Rodrigues da; Passos, Maria Cristina; Araújo, Raquel Maria Amaral; Pereira, Patrícia Aparecida Pimenta; Passos, Maria Cristina; Cunha, Luciana Rodrigues da
Os Bancos de Leite Humano (BLH) são responsáveis pela captação, processamento e armazenamento em condições seguras do leite humano doado. Sabe-se que essas etapas de manipulação do leite humano (LH) podem interferir na sua qualidade nutricional e funcional, no entanto, as evidências científicas ainda não são bem esclarecidas. Assim, o objetivo desse trabalho foi avaliar o efeito das condições de armazenamento (tempos/temperaturas de congelamento e efeito da incidência de luz) sobre os teores de retinol e a capacidade antioxidante total (CAT) do LH, bem como a sua qualidade microbiológica. Na primeira etapa do estudo foi avaliado o efeito de diferentes tempos e temperaturas de congelamento sobre a CAT no leite humano cru (LHC) por dois métodos in vitro (ABTS e DPPH). Na segunda etapa foi avaliado o impacto da luz, conferido pela utilização de diferentes frascos de vidro (transparente, transparente recoberto por papel alumínio e âmbar) sobre os teores de retinol e a CAT no leite humano pasteurizado (LHP). A qualidade microbiológica do LHC doado pelas 10 doadoras que participaram do estudo foi avaliada por meio das seguintes análises realizadas em triplicata: contagens de Staphylococcus aureus, coliformes totais e termotolerantes, mesófilos aeróbios totais, e fungos e leveduras. Também foi aplicado um questionário e um checklist para verificar a conformidade dos procedimentos de extração e armazenamento do leite adotados pelas doadoras. De acordo com a análise de ABTS, os níveis de CAT aumentaram significativamente durante o tempo de armazenamento nas 3 temperaturas avaliadas, ao contrário da análise por DPPH em que os níveis diminuíram significativamente com o tempo de armazenamento nas 3 temperaturas, porém as duas análises indicaram que as menores temperaturas preservaram mais a CAT. Com relação ao efeito da incidência de luz por meio dos três diferentes tipos de frascos avaliados, verificou-se que não houve interferência significativa por nenhum deles na CAT. Já em relação aos teores de retinol, verificou-se que os frascos âmbar e transparente recoberto por papel alumínio permitiram maior estabilidade do retinol contido no LH. De acordo com os resultados microbiológicos, verificou-se o crescimento de todos os microrganismos estudados nos leites de todas as doadoras e, em alguns casos, acima dos limites considerados seguros. Os resultados do checklist mostram que alguns procedimentos higiênico-sanitários necessários não estão sendo realizados de forma criteriosa, sendo importante a adequada orientação das mães quanto a esses aspectos de higiene.