Navegando por Autor "Ceron, Carla Speroni"
Agora exibindo 1 - 12 de 12
Resultados por página
Opções de Ordenação
Item Antimicrobial stewardship for surgical antibiotic prophylaxis and surgical site infections : a systematic review.(2021) Martinez Sobalvarro, Joselin Valeska; Pereira Júnior, Antônio Alves; Pereira, Lucas Borges; Baldoni, André Oliveira; Ceron, Carla Speroni; Reis, Tiago Marques dosBackground Surgical site infections account for 14–17% of all healthcare-associated infections. Antimicrobial stewardship (AMS) are complementary strategies developed to optimize the use of antimicrobials. Aim to evaluate the efectiveness of AMS in promoting adherence to surgical antibiotic prophylaxis protocols in hospitalized patients, reducing surgical site infection rate and cost–beneft ratio. Method This systematic review of randomized clinical trials, non-randomized clini- cal trials and before and after studies was performed using Pubmed, Cochrane, Web of Science, Scopus, Embase, Google Scholar and ClinicalTrials.gov, in addition to reference lists of included studies. The risk of bias of studies was measured by the ROBINS-I checklist and the quality of the evidence synthesis by GRADE. Results Fourteen before and after design studies were included. In 85.7% of the studies, AMS was efective in increasing adherence to surgical antibiotic prophylaxis protocols and in 28.5%, there was reduction in surgical site infection rate. Three studies evaluated cost–beneft ratio and found a favorable impact. Eight (57%) studies were at risk of moderate bias and six had severe bias. The evaluation of the synthesis of evidence showed quality ranging from low to very low. Conclusion AMS, such as audit, feedback, education, implementation of a protocol, and a computer-assisted decision support methodology, appear to be efective in promoting adherence to surgical antibiotic prophylaxis protocols, reducing surgical site infection rate with a positive economic impact. However, more studies, particularly randomized clinical trials, are needed to improve the level of evidence of available information on AMS in order to favor decision-making.Item Ayahuasca, a psychedelic beverage, modulates neuroplasticity induced by ethanol in mice.(2022) Almeida, Carolina Aparecida de Faria; Pereira Júnior, Antônio Alves; Rangel, Jéssica Gonçalves; Pereira, Bruna Pinheiro; Costa, Karla Cristinne Mancini; Bruno, Vitor; Silveira, Gabriela Oliveira; Ceron, Carla Speroni; Yonamine, Mauricio; Camarini, Rosana; Garcia, Raphael Caio Tamborelli; Marcourakis, Tania; Torres, Larissa HelenaAlcohol use disorder needs more effective treatments because relapse rates remain high. Psychedelics, such as ayahuasca, have been used to treat substance use disorders. Our study aimed to evaluate the effects of ayahuasca on ethanol-induced behavioral sensitization (EIBS). Swiss mice received 2.2 g/kg ethanol or saline IP injections every other day across nine days (D1, D3, D5, D7, and D9), and locomotor activity was evaluated 10 min after each injection. Then, animals were treated daily with ayahuasca (corresponding to 1.76 mg/kg of N,N- dimethyltryptamine, DMT) or water by oral gavage for eight consecutive days. On the seventh day, mice were evaluated in the elevated plus maze. Then, mice were challenged with a single dose of ethanol to measure their locomotor activity. Dopamine receptors, serotonin receptors, dynorphin, and prodynorphin levels were quanti- fied in the striatum and hippocampus by blot analysis. Repeated ethanol administration resulted in EIBS. However, those animals treated with ayahuasca had an attenuated EIBS. Moreover, ayahuasca reduced the anxiogenic response to ethanol withdrawal and prevented the ethanol-induced changes on 5-HT1a receptor and prodynorphin levels in the hippocampus and reduced ethanol effects in the dynorphin/prodynorphin ratio levels in the striatum. These results suggest a potential application of ayahuasca to modulate the neuroplastic changes induced by ethanol.Item Epigenetic regulation of the N-terminal truncated isoform of matrix metalloproteinase-2 (NTT-MMP-2) and Its presence in renal and cardiac diseases.(2021) Cruz, Juliana de Oliveira; Silva, Alessandra Oliveira; Ribeiro, Jessyca Milene; Luizon, Marcelo Rizzatti; Ceron, Carla SperoniSeveral clinical and experimental studies have documented a compelling and critical role for the full-length matrix metalloproteinase-2 (FL-MMP-2) in ischemic renal injury, progressive renal fibrosis, and diabetic nephropathy. A novel N-terminal truncated isoform of MMP-2 (NTT-MMP-2) was recently discovered, which is induced by hypoxia and oxidative stress by the activation of a latent promoter located in the first intron of the MMP2 gene. This NTT-MMP-2 isoform is enzymatically active but remains intracellular in or near the mitochondria. In this perspective article, we first present the findings about the discovery of the NTT-MMP-2 isoform, and its functional and structural differences as compared with the FL-MMP-2 isoform. Based on publicly available epigenomics data from the Encyclopedia of DNA Elements (ENCODE) project, we provide insights into the epigenetic regulation of the latent promoter located in the first intron of the MMP2 gene, which support the activation of the NTT-MMP-2 isoform. We then focus on its functional assessment by covering the alterations found in the kidney of transgenic mice expressing the NTT-MMP-2 isoform. Next, we highlight recent findings regarding the presence of the NTT-MMP-2 isoform in renal dysfunction, in kidney and cardiac diseases, including damage observed in aging, acute ischemia-reperfusion injury (IRI), chronic kidney disease, diabetic nephropathy, and human renal transplants with delayed graft function. Finally, we briefly discuss how our insights may guide further experimental and clinical studies that are needed to elucidate the underlying mechanisms and the role of the NTT-MMP-2 isoform in renal dysfunction, which may help to establish it as a potential therapeutic target in kidney diseases.Item Ethanol consumption and sepsis : mechanisms of organ damage.(2021) Silva, Alessandra Oliveira; Prohaska, Clare C.; Ceron, Carla SperoniSepsis is highly prevalent, and is one of the main causes of mortality among hospitalized patients. Ethanol consumption in large quantities compromises the normal functioning of the body, leading to dysfunction of multiple different organ systems. The association between sepsis and ethanol is not fully understood, but it is well accepted that ethanol consumption plays a role in the development of sepsis. Both sepsis and ethanol cause inflammatory dysfunction and promote oxidative stress. Antioxidant agents may be highly relevant targets to abrogate the effects of sepsis in patients who also consume large amounts of ethanol. This review focuses on presenting the main mechanisms involved between sepsis and ethanol consumption, and to describe the main antioxidants that have been used as therapeutic agents.Item Nicotine exposure through breastfeeding affects brainderived neurotrophic factor and synaptic proteins levels in the brain of stressed adult female mice.(2022) Pereira Júnior, Antonio Alves; Amorim, Gabriel Estevam Santos de; Garcia, Raphael Caio Tamborelli; Ribeiro, Jessyca Milene; Silva, Alessandra Oliveira; Almeida, Carolina Aparecida de Faria; Ceron, Carla Speroni; Ruginsk, Silvia Graciela; Rodrigues, José Antunes; Elias, Lucila Leico Kagohara; Dias, Marcos Vinícios Salles; Marcourakis, Tania; Torres, Larissa HelenaNicotine has been used during pregnancy and lactation as a tobacco harm reduction strategy. However, it is unclear whether nicotine exposure during a critical development period negatively impacts stress responses in adulthood. This study investigated how nicotine, administered via breastfeeding, affects the brain-derived neurotrophic factor (BDNF), synaptic proteins levels, and anxiety-like behavior in adult female mice subjected to stress. Female Swiss mice were exposed to saline or nicotine (8 mg/kg/day) through breastfeeding between their fourth and 17th postnatal days (P) via implanted osmotic mini pumps. The unpredictable chronic mild stress (UCMS) protocol was performed during their adulthood (P65) for 10 consecutive days, followed by the elevated plus maze (EPM) test 1 day after the protocol. Animals were euthanized and their blood, collected for plasma corticosterone measurements and their brain structures, dissected for BDNF and synaptic proteins analyses. We found no significant differences in corticosterone levels between groups (Saline/Nonstress, Nicotine/Non-stress, Saline/Stress, and Nicotine/Stress). The UCMS protocol hindered weight gain. Mice exposed to nicotine through breastfeeding with or without the UCMS protocol in adulthood showed higher grooming and head dipping frequency; decreased BDNF levels in cerebellum and striatum; increased postsynaptic density protein 95 (PSD-95), synapsin I, and synaptophysin levels in cerebellum; and decreased PSD-95 and synapsin I levels in brainstem. Our results indicate that nicotine exposure through breastfeeding leads to long-lasting behavioral effects and synaptic protein changes, most of which were independent of the UCMS protocol, even after a long nicotine-free period, highlighting the importance of further studies on nicotine exposure during development.Item No effect of prior Dengue virus 1 infection in mouse dams on long-term behavioral profiles in offspring infected with Zika virus during gestation.(2020) Costa, Karla Cristinne Mancini; Brancaglion, Gustavo Andrade; Almeida, Carolina Aparecida de Faria; Amorim, Gabriel Estevam Santos de; Veloso, Luciana Lopes; Lião, Lucas da Silva; Souza, Gabriel Augusto Pires de; Pereira, Bruna Pinheiro; Angelo, Marilene Lopes; Ruginsk, Silvia Graciela; Brandão, Wesley Nogueira; Marcourakis, Tania; Ceron, Carla Speroni; Coelho, Luiz Felipe Leomil; Torres, Larissa HelenaZika virus (ZIKV) is a mosquito-borne Flavivirus structurally and antigenically related to Dengue virus (DENV). Zika virus has been associated with congenital anomalies and most ZIKV outbreaks have occurred in endemic areas of DENV. The present study investigated the effects of prior DENV serotype 1 (DENV1) immunity in immunocompetent female Swiss mice on gestational ZIKV infection in offspring. Physical/reflex development, locomotor activity, anxiety, visual acuity, and brain-derived neurotrophic factor (BDNF) levels were evaluated in offspring during infancy and adolescence. Anti-DENV1 and anti-ZIKV antibodies were detected in sera of the progenitors, whereas no ZIKV genomes were detected in the offspring brain. Pups from dams with only DENV1 immunity presented alterations of physical/reflex development. Pups from all infected dams exhibited time- related impairments in locomotor activity and anxiolytic-like behavior. Offspring from DENV/ZIKV-infected dams exhibited impairments in visual acuity during infancy but not during adolescence, which was consistent with morphometric analysis of the optic nerve. Pups from DENV1-, ZIKV-, and DENV/ZIKV-infected dams exhibited a decrease in BDNF levels during infancy and an increase during adolescence in distinct brain regions. In summary, we found no influence of prior DENV1 immunity on gestational ZIKV infection in offspring, with the exception of alterations of early visual parameters, and an increase in BDNF levels in the hippocampus during adolescence.Item Oral pregabalin is effective as preemptive analgesia in abdominal hysterectomy - a randomized controlled trial.(2022) Silva, Fabrício Gomes da; Podestá, Márcia Helena Miranda Cardoso; Silva, Thayná Coelho; Barros, Carlos Marcelo de; Carvalho, Breno Fialho Vitarelli de; Reis, Tiago Marques dos; Esposito, Milena Carla; Marrafon, Danielle Aparecida Ferreira de Oliveira; Nogueira, Denismar Alves; Diwan, Sudhir; Ceron, Carla Speroni; Torres, Larissa HelenaPostoperative pain is one of the main negative symptoms resulting from surgery and the use of new methods to control this symptom is of ever-increasing relevance. Opioid-sparing strategies, such as multimodal analgesia, are trends in this scenario. Pregabalin is a well-established treatment for neuropathic pain; however, it is still controversial in the surgical context for postoperative analgesia. This study investigated the effect of pregabalin on postoperative analgesia in patients undergoing abdominal hysterectomy. It is a prospective, randomised, double-blind, placebo-controlled clinical trial. Female patients undergoing abdominal hysterectomy were randomised to use pregabalin (group P1), 300 mg orally 2 h before surgery, or identical placebo pills (group P0). The main outcome includes the postoperative pain index by visual analogue scale (VAS) and McGill's pain questionnaire. Secondary outcomes include opioid consumption and the presence of adverse effects. A value of p < 0.05 was used to reject type I error. Fifty-five patients were randomised amongst the groups. Patients in group P1 had lower pain rates by VAS scale, both at rest and in active motion, than group P0. In McGill's questionnaire, patients from group P1 also had lower pain rates (12 28.5). There was approximately twice as much opioid consumption amongst patients in group P0. Regarding side effects, there was a difference between the two groups only for dizziness, being more incident in group P1. This study suggests that pregabalin is an important adjuvant drug in treating postoperative pain in patients with abdominal hysterectomy.Item Potential roles of visfatin/NAMPT on endothelial dysfunction in preeclampsia and pathways underlying cardiac and vascular remodeling.(2022) Ceron, Carla Speroni; Pereira, Daniela Alves; Sandrim, Valeria Cristina; Luizon, Marcelo RizzattiItem Protective effects of kefir against unpredictable chronic stress alterations in mice central nervous system, heart, and kidney.(2022) Silva, Alessandra Oliveira; Ribeiro, Jessyca Milene; Patrocínio, Talita Bárbara; Amorim, Gabriel Estevam Santos de; Pereira Júnior, Antônio Alves; Angelo, Marilene Lopes; Paula, Fernanda Borges de Araújo; Oliveira, Nelma de Mello Silva; Ruginsk, Silvia Graciela; Rodrigues, José Antunes; Elias, Lucila Leico Kagohara; Dias, Marcos Vinícios Salles; Torres, Larissa Helena; Ceron, Carla SperoniKefir is a probiotic mixture with anxiolytic and antioxidant properties. Chronic stress can lead to anxiety disorders and increase oxidative damage in organs such as the heart and kidney. In this study, we examined whether kefir ameliorates the anxiety-like behavior of mice submitted to chronic unpredictable stress (CUS) by modulating brain-derived neurotrophic factor (BDNF) and corticosterone levels and whether kefir modifies the oxidative parameters in the heart and kidney of mice. Male Swiss mice received kefir (0.3 mL/100 g/day) or milk for 30 days (gavage). On the 10th day, the mice were submitted to CUS. Behavioral analysis was performed using the elevated plus maze and forced swimming tests. BDNF levels were analyzed in brain tissues. Heart and kidney superoxide dismutase (SOD), catalase, glutathione (GSH), thiobarbituric acid reactive substances (TBARS), 3-nitrotyrosine, metalloproteinase-2 (MMP-2), and plasma corticosterone were evaluated. Kefir reverted the CUS-induced decrease in the time spent in the open arms, the increase in grooming frequency, and decrease in the head dipping frequency, but not the reduced immobility time. CUS decreased the cerebellum BDNF levels and increased corticosterone levels, which were restored by Kefir. Neither catalase and SOD activities nor GSH, TBARS, 3-nitrotyrosine, and MMP-2 were modified by CUS in the heart. In the kidney, CUS increased 3-nitrotyrosine and MMP-2. Kefir increased the antioxidant defense in the heart and kidney of control and CUS mice. These results suggest that kefir ameliorated CUS-induced anxiety-like behavior by modulating brain BDNF and corticosterone levels. Kefir also increased the antioxidant defense of mice heart and kidney.Item Pyrrolidine dithiocarbamate reduces alloxan-induced kidney damage by decreasing nox4, inducible nitric oxide synthase, and metalloproteinase-2.(2020) Pereira, Bruna Pinheiro; Valle, Gabriel Tavares do; Salles, Bruno César Côrrea; Costa, Karla Cristinne Mancini; Angelo, Marilene Lopes; Torres, Larissa Helena Lobo; Novaes, Rômulo Dias; Ruginsk, Silvia Graciela; Tirapelli, Carlos Renato; Paula, Fernanda Borges de Araújo; Ceron, Carla SperoniWe examined the effect of the NFκB inhibitor pyrrolidine-1-carbodithioic acid (PDTC) on inducible nitric oxide synthase (iNOS), matrix metalloproteinase-2 (MMP-2) activity, and oxidative and inflammatory kidney damage in alloxan-induced diabetes. Two weeks after diabetes induction (alloxan-130 mg/kg), control and diabetic rats received PDTC (100 mg/kg) or vehicle for 8 weeks. Body weight, glycemia, urea, and creatinine were measured. Kidney changes were measured in hematoxylin/eosin sections and ED1 by immunohistochemistry. Kidney thiobarbituric acid reactive substances (TBARS), superoxide anion (O2−), and nitrate/nitrite (NOx) levels, and catalase and superoxide dismutase (SOD) activities were analyzed. Also, kidney nox4 and iNOS expression, and NFkB nuclear translocation were measured by western blot, and MMP-2 by zymography. Glycemia and urea increased in alloxan rats, which were not modified by PDTC treatment. However, PDTC attenuated kidney structural alterations and macrophage infiltration in diabetic rats. While diabetes increased both TBARS and O2 − levels, PDTC treatment reduced TBARS in diabetic and O2 − in control kidneys. A decrease in NOx levels was found in diabetic kidneys, which was prevented by PDTC. Diabetes reduced catalase activity, and PDTC increased catalase and SOD activities in both control and diabetic kidneys. PDTC treatment reduced MMP-2 activity and iNOS and p65 NFκB nuclear expression found increased in diabetic kidneys. Our results show that the NFκB inhibitor PDTC reduces renal damage through reduction of Nox4, iNOS, macrophages, and MMP-2 in the alloxan-induced diabetic model. These findings suggest that PDTC inhibits alloxan kidney damage via antioxidative and anti-inflammatory mechanisms.Item The role of IL‐10 in immune responses against Pseudomonas aeruginosa during acute lung infection.(2020) Belo, Valéria Aparecida; Pereira, Jéssica Assis; Souza, Sara Franchin Duarte de; Tana, Fernanda de Lima; Pereira, Bruna Pinheiro; Lopes, Débora de Oliveira; Ceron, Carla Speroni; Novaes, Rômulo Dias; Corsetti, Patrícia Paiva; Almeida, Leonardo Augusto dePseudomonas aeruginosa is considered an opportunistic pathogen of great clinical importance. The clearance of this bac- terium occurs through recognition of the pathogen by innate immune system receptors, leading to a lung infammatory response. However, this response must be controlled via immunoregulatory pathways. In this study, we evaluate the role of endogenous murine IL-10 after acute infection with the virulent strain P. aeruginosa PA14. To assess the role of IL-10, intratracheal infection with the PA14 strain was performed in C57BL/6 or IL-10 KO mice. The PA14 strain was recovered in both types of animals, although IL-10 KO mice presented a higher number of viable bacteria in the lung when compared to the C57BL/6 group. Histopathological and stereological analyses showed that IL-10 KO mice had higher tissue damage and infammatory infltrate when compared to control animals. The activity of MMP-9 but not MMP-2, as well as IL-6 and TNF-α expression, were augmented in the lungs of infected animals and was much more evident in IL-10 KO animals when compared to the other analyzed groups. This work indicates that endogenous IL-10 control P. aeruginosa infection, the expression of pro-infammatory genes, MMP-9 activity and histopathological processes of the infectious process in question.Item The role of nitric oxide in renovascular hypertension : from the pathophysiology to the treatment.(2022) Pereira, Bruna Pinheiro; Vale, Gabriel Tavares do; Ceron, Carla SperoniRenovascular hypertension is one of the most relevant causes of secondary hypertension, mostly caused by atherosclerotic renovascular stenosis or fbromuscular dysplasia. The increase in angiotensin II production, oxidative stress, and formation of peroxynitrite promotes the decrease in nitric oxide (NO) availability and the development of hypertension, renal and endothelial dysfunction, and cardiac and vascular remodeling. The NO produced by nitric oxide synthases (NOS) acts as a vasodilator; however, endothelial NOS uncoupling (eNOS) also contributes to NO reduced availability in renovascular hypertension. NO donors and NO-derived metabolites have been investigated in experimental renovascular hypertension and have shown promissory efects in attenuating blood pressure and organ damage in this condition. Therefore, understand- ing the role of decreased NO in the pathophysiology of renovascular hypertension promotes the study and development of NO donors and molecules that can be converted into NO (such as nitrate and nitrite), contributing for the treatment of this condition in the future.