Navegando por Autor "Castro, Maria Laura da Cruz"
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Item Efeito da doxiciclina na melhora cognitiva de animais com sepse polimicrobiana : papel da metaloproteinase cerebral na regulação das vias neuro inflamatórias e oxidativas.(2023) Castro, Maria Laura da Cruz; Costa, Daniela Caldeira; Calsavara, Allan Jefferson Cruz; Costa, Daniela Caldeira; Calsavara, Allan Jefferson Cruz; Barichello, Tatiana; Santos, Orlando David Henrique dos; Gomes, Sílvia de PaulaIndivíduos que sobrevivem à sepse podem apresentar sequelas neurológicas com diminuição da função cognitiva. Um dos mecanismos responsáveis por esta diminuição da função cognitiva é o aumento da atividade das metaloproteinases da matriz (MMPs). A doxiciclina é um antibiótico da classe das tetraciclinas e um potente inibidor de MMPs. Assim, o objetivo desse trabalho foi avaliar o efeito da doxiciclina (um inibidor de MMPs) na possível melhora cognitiva de camundongos com sepse polimicrobiana e os seus mecanismos subjacentes. Foram utilizados 183 camundongos isogênicos C57BL/6 machos, com 8 a 12 semanas de idade distribuídos em cinco grupos: Grupo 1: animais submetidos à cirurgia branca e tratados com soro fisiológico 0,9% (SHAM) (n=27); Grupo 2: animais submetidos à cirurgia de ligadura e perfuração do ceco (CLP) e tratados com soro fisiológico 0,9% (n=49); Grupo 3: CLP tratados com doxiciclina (20 mg/kg/dia) (DOX20) (n=38); Grupo 4: CLP tratados com doxiciclina (40 mg/kg/dia) (DOX40) (n=32); Grupo 5: CLP tratados com ceftriaxona (400 mg/kg/dia) (CTX) (n=37). Os tratamentos foram realizados por meio de uma injeção subcutânea diária durante o período de cinco dias. No quarto dia de tratamento os animais foram submetidos aos testes cognitivos. O tratamento com a doxiciclina foi capaz de reduzir os níveis de TNF e VEGF no córtex cerebral, inibir MMP-9, melhorar o perfil redox, por meio do aumento da atividade de SOD e catalase e diminuição do dano oxidativo lipídico, além de aumentar os níveis de BDNF no hipocampo e melhorar a cognição dos animais. Além disso, a doxiciclina interagiu de maneira mais efetiva com a MMP-9 do que a ceftriaxona no estudo de modelagem molecular. Portanto, nossos resultados sugerem que a doxiciclina por meio da sua capacidade de inibir MMPs e dos seus mecanismos imunomoduladores e antioxidantes é um potencial fármaco para o tratamento do déficit cognitivo em indivíduos que sobrevivem à sepse.Item EPA/DHA and linseed oil have different effects on liver and adipose tissue in rats fed with a high-fat diet.(2022) Dias, Bruna Vidal; Gomes, Sttefany Viana; Castro, Maria Laura da Cruz; Carvalho, Luana Cristina Faria; Breguez, Gustavo Silveira; Souza, Débora Maria Soares de; Ramos, Camila de Oliveira; Sant’Ana, Marcella Ramos; Nakandakari, Susana Castelo Branco Ramos; Araujo, Carolina Morais; Guimaraes, Andrea Grabe; Silva, André Talvani Pedrosa da; Carneiro, Cláudia Martins; Cintra, Dennys Esper Corrêa; Costa, Daniela CaldeiraThe incidence of cardiovascular diseases and metabolic disorders has increased worldwide. Clinical and exper- imental research has shown that the consumption of ω-3 FAs can be beneficial to metabolism in several ways, as they can act on metabolic pathways. Our objective was to evaluate the effect of treatment with linseed oil, a vegetable oil rich in alpha-linolenic acid, and EPA and DHA in different proportions (3:1 EPA:DHA, and 1:3 EPA: DHA), on the metabolic disorders induced by a high-fat diet (20 % lipids) in rats for 2 weeks, after 18 weeks of consumption of a high-fat diet. In 18 weeks, the high-fat diet increased blood glucose, systolic blood pressure, triglyceride concentration in the liver and adipose tissue, and impaired insulin sensibility without interfering in the weight of the animals. All treatments were effective in reducing the deposition of hepatic type III collagen, the proportion of ω-6/ω-3 in the liver and WAT (white adipose tissue), the proportion of area/number of adi- pocytes, and the gene expression of the ACC, FAS, and CPT1 enzymes. In addition, treatment with EPA and DHA reduced blood glucose, serum TNF-α concentration, amount of liver fat, degree of microsteatosis and type I collagen deposition in the liver, deposition of type I and III collagen in TA, gene expression of the transcription factor SREBP-1c, and increased hepatic binucleation. EPA in major proportion was more effective in reducing the area of adipocytes, hepatic triglyceride concentration, PPAR-α expression, and WAT fat weight. DHA in a major proportion reduced the concentration of MCP1 in WAT. LO treatment did not have any isolated effects. We concluded that EPA and DHA were more effective in treating metabolic damage than treatment with LO, leading to a more favorable metabolic profile.Item Long-term e-cigarette aerosol exposure causes pulmonary emphysema in adult female and male mice.(2023) Rodriguez Herrera, Andrea Jazel; Souza, Ana Beatriz Farias de; Castro, Thalles de Freitas; Machado Júnior, Pedro Alves; Marcano Gomez, Elena Cecilia; Menezes, Tatiana Prata; Castro, Maria Laura da Cruz; Silva, André Talvani Pedrosa da; Costa, Daniela Caldeira; Cangussú, Silvia Dantas; Bezerra, Frank SilvaThis study aimed to evaluate long-term exposure to conventional cigarette smoke (CC) and electronic cigarette (EC) aerosol in adult male and female C57BL/6 mice. Forty-eight C57BL/6 mice were used, male (n = 24) and female (n = 24), both were divided into three groups: control, CC and EC. The CC and EC groups were exposed to cigarette smoke or electronic cigarette aerosol, respectively, 3 times a day for 60 consecutive days. Afterwards, they were maintained for 60 days without exposure to cigarettes or electronic cigarette aerosol. Both cigarettes promoted an influx of inflammatory cells to the lung in males and females. All animals exposed to CC and EC showed an increase in lipid peroxidation and protein oxidation. There was an increase of IL-6 in males and females exposed to EC. The IL-13 levels were higher in the females exposed to EC and CC. Both sexes exposed to EC and CC presented tissue damage characterized by septal destruction and increased alveolar spaces compared to control. Our results demonstrated that exposure to CC and EC induced pulmonary emphysema in both sexes, and females seem to be more susceptible to EC.