Navegando por Autor "Carvalho, Rodrigo Franco de"
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Item Can established cultured papilloma cells harbor bovine papillomavirus?(2008) Campos, Sérgio Roberto de Souza Leão da Costa; Trinidade, C.; Ferraz, Oilita Pereira; Silva, Dalton Giovanni Nogueira da; Lima, Angélica Alves; Caetano, Heloisa Vasconcellos Amaral; Carvalho, Rodrigo Franco de; Birgel Junior, Eduardo Harry; Dagli, Maria Lucia Zaidan; Mori, Enio; Brandão, Paulo Eduardo; Richtzenhain, Leonardo José; Beçak, Willy; Stocco, Rita de CássiaPapillomaviruses have been reported to be very dif¬ficult to grow in cell culture. Also, there are no descriptions of cell cultures from lesions of bovine cutaneous papillomatosis, with identification of different bovine papilloma virus (BPV) DNA se¬quences. In the present report, we describe primary cell cultures from samples of cutaneous lesions (warts). We investigated the si¬multaneous presence of different BPV DNA sequences, comparing the original lesion to different passages of the cell cultures and to peripheral blood. BPV 1, 2 and 4 DNA sequences were found in lesion samples, and respective cell cultures and peripheral blood, supporting our previous hypothesis of the possible activity of these sequences in different samples and now also showing how they can be maintained in different passages of cell cultures.Item Human papillomavirus infection in Brazilian women with normal cervical cytology.(2012) Miranda, Priscila Mayrink de; Pitol, Bruna Caroline Vieira; Moran, Mariana Soares; Silva, Nayara Nascimento Toledo; Felix, Priscilla Muller; Lima Filho, José Luiz de; Carneiro, Cláudia Martins; Silva, Ismael Dale Cotrim Guerreiro da; Carvalho, Rodrigo Franco de; Lima, Angélica Alves; Beçak, Willy; Stocco, Rita de CássiaWe examined the prevalence of human papillomavirus (HPV) infection in a sample of Brazilian women presenting normal cervical cytology. Possible interactions between patient characteristics and HPV infection were analyzed in order to provide background data to improve cervical cancer screening and prophylaxis. Cervical samples of 399 women, received for routine evaluation in the Health Department of Ouro Preto, MG, Brazil, were subjected to HPV-DNA testing by PCR with MY09/11 primers. HPV-positive specimens were typed by RFLP. A structured epidemiological questionnaire was administered to each woman. HPV prevalence among these cytologically normal women was 11%. Twelve viral types were detected, the most common being HPV-16, -6, -61, -83, and -66. HPV was more prevalent in younger women; high-risk viral types were detected in 61% of the infected women and 27% of the infected women had multiple HPV infections. Significant associations of HPV infection were found with age, literacy, residence, marital status, lifetime number of sexual partners, and parity. We detected a great diversity of HPV types in women with normal cytology. This kind of information about local populations is useful for HPV prevention and vaccination strategies.Item Persistence or clearance of human papillomavirus infections in women in Ouro Preto, Brazil.(2013) Miranda, Priscila Mayrink de; Silva, Nayara Nascimento Toledo; Pitol, Bruna Caroline Vieira; Silva, Ismael Dale Cotrim Guerreiro da; Lima Filho, José Luiz de; Carvalho, Rodrigo Franco de; Stocco, Rita de Cássia; Beçak, Willy; Lima, Angélica AlvesPersistent high-risk (HR) human papillomavirus (HPV) infection is necessary for development of precursor lesions and cervical cancer.We investigate persistence and clearance of HPV infections and cofactors in unvaccinated women. Cervical samples of 569 women (18–75 years), received for routine evaluation in the Health Department of Ouro Preto, Brazil, were collected and subjected to PCR (MY09/11 orGP5+/6+ primers), followed by RFLP or sequencing. All women were interviewed to collect sociodemographic and behavioral information. Viral infection persistence or clearance was reevaluated after 24 months and was observed in 59.6% and 40.4% of women, respectively. HPVs 16, 33, 59, 66, 69, and 83 (HR) were the most persistent types whereas HPVs 31, 45, and 58 were less persistent. Clearance or persistence did not differ between groups infected by HPVs 18, 53, and 67. In low-risk (LR) types, HPV 6 infected samples were associated with clearance, while HPV 11, 61, 72, or 81 infected samples were persistent in the followup. No statistically significant association was detected between persistent HPV infections and sociodemographic and behavioral characteristics analyzed. To study persistence or clearance in HPV infection allows the identification of risk groups, cofactors, and strategies for prevention of cervical cancer.