Navegando por Autor "Carbajal Arízaga, Gregorio Guadalupe"

Agora exibindo 1 - 2 de 2

Effects of a lycopene-layered double hydroxide composite administration in cells and lungs of adult mice.
(2023) Carvalho, Iriane Marques de; Souza, Ana Beatriz Farias de; Castro, Thalles de Freitas; Machado Júnior, Pedro Alves; Menezes, Tatiana Prata; Dias, Andreia da Silva; Oliveira, Laser Antônio Machado de; Nogueira, Katiane de Oliveira Pinto Coelho; Silva, André Talvani Pedrosa da; Cangussú, Silvia Dantas; Carbajal Arízaga, Gregorio Guadalupe; Bezerra, Frank Silva
Lycopene is a natural compound with one of the highest antioxidant activities. Its consumption is associated with lower risks in lung cancer and chronic obstructive pulmonary disease, for example. Experimentally, a murine model demonstrated the ingestion of lycopene, which reduced the damage in lungs caused by cigarette smoke. Since lycopene is highly hydrophobic, its formulations in supplements and preparations for laboratory assays are based on oils, additionally, bioavailavility is low. We developed a lycopene layered double hydroxide (Lyc-LDH) composite, which is capable of transporting lycopene aqueous media. Our objective was to evaluate the cyto- toxicity of Lyc-LDH and the intra-cellular production of reactive oxygen species (ROS) in J774A.1 cells. Also, in vivo assays were conducted with 50 male C57BL/6 mice intranasally treated with Lyc-LDH 10 mg/kg (LG10), Lyc-LDH 25 mg/kg (LG25) and Lyc-LDH 50 mg/kg (LG50) during five days compared against a vehicle (VG) and control (CG) group. The blood, bronchoalveolar lavage fluid (BALF) and lung tissue were analyzed. The results revealed that Lyc-LDH composite attenuated intracellular ROS production stimulated with lipopolysacharide. In BALF, the highest doses of Lyc-LDH (LG25 and LG50) promoted influx of macrophages, lymphocytes, neutrophils and eosinophils compared to CG and VG. Also, LG50 increased the levels of IL-6 and IL-13, and promoted the redox imbalance in the pulmonary tissue. On the contrary, low concentrations did not produce significative effects. In conclusion, our results suggest that intranasal administration of high concentrations of Lyc-LDH in- duces inflammation as well as redox status changes in the lungs of healthy mice, however, results with low concentrations open a promising way to study LDH composites as vehicles for intranasal administration of antioxidant coadjuvants.
Understanding the synergistic antioxidant mechanism of a layered double hydroxide–lycopene composite by spectroscopic techniques.
(2023) González Rojas, Norma Alejandra; Moisés, Fermín Paul Pacheco; Cruz Ramos, José Alfonso; Bezerra, Frank Silva; Carbajal Arízaga, Gregorio Guadalupe
Lycopene is one of the most powerful natural antioxidants with demonstrated capacity to protect and reduce risks against some diseases. It has also been used as additive in polymers for packaging. Hydrophobicity is the main disadvantage of this compound, especially to prepare edible formulations and assimilation in the body; to improve this, emulsions have been formulated although they provide a low content of lycopene. For this study, we prepared a hybrid composite material with layered double hydroxides (LDHs) and 54% of lycopene. The composite is easily dispersed in water and when subjected to aqueous media to simulate a pass through stomach (pH 1.2), small intestine (pH 6.8) and large intestine (pH 7.4), where the composite released 84.5%, 92.8 and 96.7% of lycopene, respectively. The composite was analyzed in each step. The antioxidant capacity of the composite was similar to that of pure lycopene, although the content of lycopene was partial. Electron paramagnetic resonance (EPR) and X-ray photoelectron spectroscopy (XPS) indicated that the cations from LDH particles also contribute to capture unpaired electrons, thus producing a synergy in the antioxidant activity. Further, UV–vis and infrared spectroscopies demonstrated that the lycopene molecule was protected within along the pass through the three simulated media. This composite is an alternative vehicle to prepare lycopene supplements for oral consumption, maximizing the stability, and enhancing the release and therapeutic profle. This composite is also an alternative antioxidant additive for polymers or biofuels.