Navegando por Autor "Brito, Larissa Froede"
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Item Mangifera indica leaves extract and mangiferin modulate CB1 and PPARγ receptors and others markers associated with obesity.(2019) Brito, Larissa Froede; Gontijo, Douglas da Costa; Toledo, Renata Celi Lopes; Barcelos, Rafael Mazioli; Oliveira, Alaíde Braga de; Brandão, Geraldo Célio; Sousa, Lirlândia Pires de; Ribeiro, Sônia Machado Rocha; Leite, João Paulo Viana; Fietto, Luciano Gomes; Queiroz, José Humberto deThis study aimed phytochemical characterization (UPLC-DAD-MS/MS) of ethanolic extract of the leaves from Mangifera indica (EMI) and Mangiferin (MAN), analysis of the cytotoxic (MTT) and anti-inflammatory potential (expression of TNF-α) of EMI and MAN in vitro. In addition, was evaluate the effect on the mRNA expression of genes (CB1, PPARγ, adiponectin, resistin and leptin) associated with adipogenesis in adipose tissue of rats fed a cafeteria diet. Thus, wistar rats were treated by gavage with EMI and MAN for several days (according to the post and co-treatments). The adipose tissue was weighed and checked the expression of different markers by RT-PCR. The presence of MAN as major compound in EMI was verified. Both EMI and MAN were not cytotoxic, with lower EMI expression of TNF-α. Furthermore, EMI and MAN had proadipogenic action on post-treatment, while in the co-treatment, EMI attenuated the effect of adipogenesis and MAN increased the adipogenic process.Item Plasmin and plasminogen prevent sepsis severity by reducing neutrophil extracellular traps and systemic inflammation.(2023) Silva, Juliana Priscila Vago da; Zaidan, Isabella; Perucci, Luiza Oliveira; Brito, Larissa Froede; Teixeira, Lívia Cristina Ribeiro; Silva, Camila Meirelles Souza; Miranda, Thaís Cristina de; Melo, Eliza Mathias; Bruno, Alexandre Santos; Queiroz Júnior, Celso Martins; Sugimoto, Michelle Adriane Amantéa; Tavares, Luciana Padua; Ferreira, Lais Cunha Grossi; Borges, Isabela Nascimento; Schneider, Ayda Henriques; Baik, Nagyung; Silva, André Talvani Pedrosa da; Ferreira, Raphael Gomes; Alves Filho, José Carlos Farias; Nobre Junior, Vandack Alencar; Teixeira, Mauro Martins; Parmer, Robert J.; Miles, Lindsey A.; Sousa, Lirlândia Pires deSepsis is a lethal syndrome characterized by systemic inflammation and abnormal coagulation. Despite therapeutic advances, sepsis mortality remains substantially high. Herein, we investigated the role of the plasminogen/plasmin (Plg/Pla) system during sepsis. Plasma levels of Plg were significantly lower in mice subjected to severe compared with nonsevere sepsis, whereas systemic levels of IL-6, a marker of sepsis severity, were higher in severe sepsis. Plg levels correlated negatively with IL-6 in both septic mice and patients, whereas plasminogen activator inhibitor-1 levels correlated positively with IL-6. Plg deficiency render mice susceptible to nonsevere sepsis induced by cecal ligation and puncture (CLP), resulting in greater numbers of neutrophils and M1 macrophages, liver fibrin(ogen) deposition, lower efferocytosis, and increased IL-6 and neutrophil extracellular trap (NET) release associated with organ damage. Conversely, inflammatory features, fibrin(ogen), and organ damage were substantially reduced, and efferocytosis was increased by exogenous Pla given during CLP- and LPS-induced endotoxemia. Plg or Pla protected mice from sepsis-induced lethality and enhanced the protective effect of antibiotics. Mechanistically, Plg/Pla– afforded protection was associated with regulation of NET release, requiring Pla-protease activity and lysine binding sites. Plg/Pla are important host-protective players during sepsis, controlling local and systemic inflammation and collateral organ damage.