Navegando por Autor "Assis, Marieta Torres de Abreu"

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    Identification of a phylogenetically distinct orthobunyavirus from group C.
    (2011) Magalhães, Cíntia Lopes de Brito; Drumond, Betânia Paiva; Novaes, Renata Franco Vianna; Quinan, Bárbara Rezende; Magalhães, José Carlos de; Santos, João Rodrigues dos; Pinto, Carla do Amaral; Assis, Marieta Torres de Abreu; Bonjardim, Cláudio Antônio; Kroon, Erna Geessien; Ferreira, Paulo César Peregrino
    Apeu virus (APEUV) (family Bunyaviridae, genus Orthobunyavirus) was plaque purified and characterised by serological and molecular analysis. Neutralising assays confirmed cross-reactivity between purified APEUV clones and the Caraparu virus complex of group C orthobunyaviruses. Partial sequencing of the L, M and S segments of one APEUV clone (APEUV-CL5) was carried out. A phylogenetic tree constructed with the L amino acid sequences clustered APEUV-CL5 within the genus Orthobunyavirus, confirming its serological classification. Analysis of M segment sequences clustered APEUV-CL5 in the Caraparu virus complex (Group C), in agreement with serological tests and previous molecular characterisation. However, the sequence of the nucleocapsid gene (N) gave low identity values when compared to those of the group C viruses. The phylogenetic tree based on N nucleotide sequences clustered APEUV-CL5 next to the California and Bwamba groups. This remarkable S nucleotide variability suggests that APEUV-CL5 could be a genetic reassortant and that this evolutionary mechanism is present in the history of the group C viruses.