Navegando por Autor "Araujo, Wagner Wlysses Rodrigues de"
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Item Cell adhesion and growth on surfaces modified by plasma and ion implantation.(2014) Araujo, Wagner Wlysses Rodrigues de; Teixeira, Fernanda de Sá; Silva, Glenda Nicioli da; Salvadori, Daisy Maria Fávero; Salvadori, Maria Cecília Barbosa da SilveiraIn this study, we show and discuss the results of the interaction of living CHO (Chinese Hamster Ovary) cells, in terms of adhesion and growth on glass, SU-8 (epoxi photoresist), PDMS (polydimethylsiloxane), and DLC (hydrogen free diamond-like carbon) surfaces. Glass, SU-8, and DLC but not PDMS showed to be good surfaces for cell growth. DLC surfaces were treated by oxygen plasma (DLC-O) and sulfur hexafluoride plasma (DLC-F). After 24 h of cell culture, the number of cells on DLC-O was higher than on DLC-F surface. SU-8 with silver implanted, creating nanoparticles 12 nm below the surface, increased significantly the number of cells per unit area.Item Cell growth on 3D microstructured surfaces.(2016) Araujo, Wagner Wlysses Rodrigues de; Teixeira, Fernanda de Sá; Silva, Glenda Nicioli da; Salvadori, Daisy Maria Fávero; Salvadori, Maria Cecília Barbosa da Silveira; Brown, I. G.Chinese Hamster Ovary (CHO) cell cultures were grown on surfaces lithographed with periodic 3D hexagonal microcavity array morphology. The range of microcavity size (inscribed circle diameter) was from 12 μm to 560 μm. CHO cells were grown also on flat surfaces. The characterization was performed with respect to cell growth density (number of nuclei per unit area) by fluorescence opticalmicroscopy and evaluated by correlation function analysis. We found that optimum microcavity radius was 80 μm, concerning to the maximum cell growth density, being even greater than the growth density on a flat (unstructured) substrate of the same material. This finding can be important for optimization of biotechnological processes and devices.